遗传操作

本文简要综述了重组DNA技术应用于酿酒酵母遗传操作的研究进展，并指出了重组DNA技术作为新的遗传操作的手段，其应用性明显优于经典的遗传操作方法。

Furthermore , the application of recombinant DNA techniques in the genetic manipulation of S. cerevisiae is now largely preferred to the classic genetic methods .

除了具有简单的遗传操作和培养外，大肠杆菌因其本身没有自已的细胞色素酶，成为表达人类细胞色素酶P450的合适宿主。

Besides the easy genetic manipulation and cultivation , E.coli is a suitable host for the expression of CYP since it has no own CYP background .

为猪瘟病毒C株反向遗传操作系统的建立奠定了基础。

The study established a foundation for reverse genetic system of CSFV .

反向遗传操作技术产生全禽源致弱的H5N1亚型禽流感病毒的免疫保护性试验

Protective Efficacy of Attenuated H5N1 Recombinant Avian Influenza Virus by Reverse Genetics

改进遗传操作PSO算法及其在VRP中的应用

Improved Genetic Operator PSO and Its Application in VRP

一株H5N1亚型猪流感病毒的进化分析及其反向遗传操作系统的建立

Evolution Analysis and the Establishment of Reverse Genetics System of an H5N1 Swine Influenza Virus

本研究构建了水泡性口炎病毒印第安纳株（VSV-Ind）基因组全长cDNA克隆，建立了反向遗传操作系统并成功救获病毒。

The full-length genome cDNA clone of VSV Indiana strain was constructed , and the virus was generated by reverse genetic system .

这样，ES细胞就提供了一个有效的纽带，将动物基因组的体外和体内遗传操作连系起来。

Therefore , ES cells provide a unique tool linking in vitro and in vivo genetic manipulations of animal genomes .

同时通过共享技术实现对遗传操作过程中解的收敛性和均匀性进行控制，使得解朝着最优权衡曲面收敛，从而得到问题的Pareto最优解。

And convergence and equality of solutions is controlled through fitness sharing technique , which makes solutions converge to optimal trade-off surface .

而正链RNA病毒的反向遗传操作系统在病毒复制、致病机制研究，抗病毒药物筛选和新型疫苗、导向载体制备等方面是一个极其有用的工具。

Positive-strand RNA virus reverse genetics system is an useful tool in viral replication , pathogenesis research , screening antiviral drugs and new vaccines , vector-oriented areas such as the preparation .

上层GA通过树基对解空间进行划分，缩小搜索范围，同时通过改良的遗传操作，减少不可行解出现的几率；

In the upper module , GA can reduce hunting space by the way of dividing solution space , additionally , an improved GA is developed to reduce unfeasible solutions ;

但是，关于NDV耐热毒株反向遗传操作系统的成功构建至今仍未见报道。

However , there has been no report on successful building of the reverse genetic operating system of NDV thermostable strain .

最后仔细分析了AGA的遗传操作，说明AGA可由时齐Markov链来描述。

Finally , the genetic operation of AGA is analyzed carefully . It shows AGA can be described as a homogeneous Markov chain .

介绍了GEP算法的编码方式、适应度函数和遗传操作。

It introduces the encoded mode , genetic operator and fitness function of the algorithm of GEP in details .

针对模型参数识别问题，笔者又提出了两种嵌入了基本位遗传操作算子的GA新策略，分别用于识别经典时序模型的参数和优化人工神经网络模型（简称ANN）的权重。

In terms of indemnifying model coefficients , the author develops two GA versions embedded the two bit genetic operators , for evaluating classical time series models and the weights of ANN .

反向遗传操作作为一种新兴技术在RNA病毒的研究中发挥着重要作用，本文介绍了RNA病毒反向遗传学的研究方法以及RNA病毒反向遗传技术的最新研究进展。

Reverse genetics is a newly developed technique in the study of RNA viruses . This paper described the basic methods of constructing infectious molecular clone and the recent progress in reverse genetics of RNA viruses .

与采用相同遗传操作的GA比较，基于GPSO模型的算法解的质量与收敛稳定性提高，同时计算费用显著降低。

In contrast to the GA with the same genetic operator , GPSO based algorithm converges consistently with better TSP solutions and saves computational cost significantly .

利用混沌变量的遍历性，将其引入到遗传操作，设计了一种混沌遗传算法（ChaosGeneticAlgorithms，CGA）。

The EGA is applied to estimate the kinetic model parameter and perfect results are obtained . ( 4 ) A chaos genetic algorithms ( CGA ) is designed through introducing chaos variable to genetic algorithm by the full use of its ergodic property .

在突变株的构建过程中，我们发现由于S.aureus细胞壁厚而致密而且穿梭重组质粒较大，使用目前的常规转化方法无法导入外源性DNA，限制了遗传操作的进行。

In the process of mutant construction , we found it difficult to transfer exogenous DNA into S. aureus cells during transformation because of the thick compact cell wall of S. aureus and the large shuttle vector we used .

自发突变或遗传操作（敲除或下调）造成的MSTN失活，已经被报道可以增加许多种动物的骨骼肌数量。

Dysfunction of MSTN gene either by natural mutation or induced through genetic manipulation ( knock out or knock down ) has been reported to increase the muscle mass in mammalian species .

该算法除了常规遗传操作以外，设计了Pareto解集过滤器和小生境技术，求得均匀分布的Pareto最优解集，较好地解决了货位分配多目标优化问题，并为AS/RS提供多种动态优化解决方案。

The approach adds Pareto solution sets and Niche technology besides traditional operators . It can search the optimum solution sets that distribute uniformly . The approach ensures storage location assignment optimization and offers an optimization decision making scheme for AS / RS. 3 .

该算法根据MRST问题的特点，采用了树形结构编码方案以及相应的遗传操作方法，在群体设定时均匀划分空间，依据遗传群体的环境参量动态地调整遗传算法的进化策略；

The algorithm uses tree like genetic operation , partitions the solution space uniformly and tunes the evolution strategies adaptively based on the genetic environment parameters .

在MGGA中采用的染色体一改传统的矩阵编码方式，用一种与自然界中生物染色体相吻合的一维结构体编码方式，使遗传操作更直观、方便。

This coding manner , which is different from the traditional matrix coding manner , makes GA more convenient and clear .

IEP采用两层编码的染色体结构，上层结构基因串是二进制编码，可进行交叉和变异的遗传操作，下层参数基因串是实数编码，只进行EP的变异操作；

IEP makes an improvement on description of gens of chromsome using two layer encoding . The upper configuration gens in binary coded can use genetic operators of crossover and mutation , the lower parameter gens in real coded can only use EP mutation operator .

遗传操作算子取值分析。

( 3 ) The span analysis of the genetic operators .

均匀设计和遗传操作并行的遗传算法

A Genetic Algorithm Based on Uniform Design Paralleled with Genetic Operation

类胡萝卜素生物合成途径及其控制与遗传操作

Carotenoid biosynthetic pathway and its control , and genetic manipulation

球形芽孢杆菌杀蚊毒素蛋白及其遗传操作研究进展

Mosquito larvicidal toxins of Bacillus sphaericus and their genetic manipulation

一种组合遗传操作的实数编码遗传算法

A Real - coded Genetic Algorithm with Genetic Operation Combination

一种基于遗传操作的神经网络混合训练算法

A Mixed Algorithm Training Neural Network Based On Genetic Operation