逆转录

逆转录：利用耐热性逆转录酶和随机引物，逆转录各成员的RNA。

Reverse transcription was performed with hot-resistance reverse transcriptase and random primers .

HBV通过特有的逆转录反应进行复制。

HBV replicates via a unique reverse transcription process .

C型逆转录病毒和自身免疫病的关系

Retrovirus with Type C. Morphology and Autoimmune Disease

C型逆转录病毒与人类白血病关系的探讨

Retrovirus with Type C.Morphology and Human Leukemia

由此可以得出结论：在其它条件相同时，用逆转录产物做PCR的退火温度低于常规peR，比常规pCR低4oC左右。

So the RT-PCR annealing temperature was about lower 4C than normal PCR .

逆转录病毒介导DNA修复蛋白在人骨髓细胞中的表达和抗性研究

Expression and drug resistance of human MGMT gene in hemopoietic cells mediated by bicistronic retroviral vector

应用逆转录套式PCR检测新城疫病毒核酸

Detection of Newcastle Disease Virus by RT - nested PCR

甲状腺肿瘤端粒酶逆转录酶mRNA的表达

Expression of mRNA of telomerase reverse transcriptase in thyroid neoplasms

差异显示逆转录PCR技术在医学真菌研究中的应用

Application of Differential Display Reverse Transcription-PCR in Medical Mycology

应用循环逆转录PCR技术检测丙型肝炎病毒RNA

Detection of Hepatitis C Virus RNA by Circulatory Reverse Transcription-PCR

应用原位逆转录PCR检测石蜡切片中HCVRNA

Detection of HCV RNA in paraffin-embedded tissues using in situ RT-PCR

DNA去甲基化对肝细胞人端粒酶逆转录酶上调的研究

DNA Demethylation induced by 5 ' - aza-dC Upregulates human telomerase reverse transcriptase in liver cell line SMMC-7721

乙型肝炎病毒C基因逆转录病毒载体的构建及在永生化人外周血B细胞中的表达

Construction of hepatitis B virus C gene retroviral vector and its expression in immortalized human peripheral blood B cell lines

羊开口提取物的分离及对艾滋病毒逆转录酶和DNA多聚酶活性的抑制作用

Differential Inhibitory Effects and Separation of Melastoma normale Extracts on the Activities of HIV Reverse Transcriptase and DNA Polymerase

单细胞逆转录PCR研究方法及其应用

Single cell RT-PCR technique and its application

结论：逆转录PCR技术是获得目的基因的有效方法。

Conclusion : Reversible transcription PCR technique is an effective method for obtaining definite gene .

原位逆转录PCR检测干细胞因子mRNA在白血病细胞的表达

Detection of stem cell factor mRNA expression in leukemic cells by in situ reverse transcriptase-PCR

胃癌中人端粒酶逆转录酶mRNA的实时定量检测

Quantification of human telomerase reverse transcriptase mRNA in gastric cancer with real-time fluorescent quantitative RT-PCR

谷胱甘肽S－转移酶pi基因与逆转录病毒载体的重组体构建

Construction of Recombinant Glutathione S-transferase pi cDNA and Retrovirus Vector

HBSAg及B（7-1）双表达逆转录病毒载体的构建及其在真核细胞中表达

Construction and expression in eukaryotic cells of HBsAg and B_ ( 7-1 ) coexpression retroviral vector

逆转录病毒载体介导双靶区乙型肝炎病毒反义RNA模型的构建

Construction of recombinant retrovirus vector carrying dual target antisense RNA of hepatitis B virus

人周围血单核细胞载脂蛋白E基因表达的竞争性逆转录-聚合酶链反应定量分析

A Competitive Reverse Transcription-Polymerase Chain Reaction to Study Quantification of Apolipoprotein E Gene Expression in Human Monocyte-derived Macrophages

目的建立逆转录病毒载体介导的人凝血因子Ⅷ（FⅧ）体外高效表达体系。

Objective To develop a retroviral mediated high efficient expression system of human coagulation factor ⅷ .

【结论】以逆转录聚合酶链反应方法成功地构建了人瘦素基因cDNA克隆。

Conclusions The human leptin gene cDNA clone was constructed by RT PCR .

用共扩增逆转录定量PCR方法测定间质胶原酶基因表达水平。

Interstitial collagenase gene expression level was mea-sured by quantitative analysis of reverse transcription polymerase chain reaction .

RT-PCR方法检测HBV的DNA多聚酶/逆转录酶P基因在PBMCs中的mRNA表达。

MRNA expressions of gene P in PBMCs in HBV were detected through RT-PCR method .

反义人类端粒酶RNA逆转录病毒载体构建及其对结直肠癌细胞的抑制作用

Construction of antisense human telomeric RNA retroviral vector and its inhibition on colorectal cancer cells

逆转录-半套式聚合酶链反应检测汉坦病毒RNA及其临床初步应用

Detection of Hantavirus by reverse transcriptase semi nested polymerase chain reaction and its preliminary application

快速提取肠道病毒RNA用于逆转录聚合酶链反应

Rapid extraction of enterovirus RNA for RT-PCR

目的：研究双靶区反义RNA的逆转录病毒载体的转染、表达及其抗乙肝病毒（HBV）的作用。

Objective : To study the effects of dual target antisense RNA against HBV infection .