转基因动物

目的利用已建立的APP转基因动物模型，探讨NO与细胞凋亡机制。

Objective To inquire into the principles governing NO and in-situ apoptosis by using the APP transgenic animal models .

神经系统退行性疾病相关的tau蛋白及其转基因动物模型

Tau protein related to neurodegenerative diseases and transgenic animal models

外源DNA与精子相互作用后的定位及内化率是精子载体法制备转基因动物的关键环节。

Exogenous DNA localization and the frequency of spermatozoa carrying exogenous DNA after sperm / DNA co-culture are key to a successful sperm mediated-gene transfer ( SMGT ) .

目的：探索以精原细胞作为载体建立带乙肝病毒X基因的树鼩转基因动物模型的可行性。

Objective : To investigate the possibility of using the spermatogonial cells as a vector to establish trans-gene animal model of tree shrew with HBV X gene .

利用精子载体法制备转基因动物是一种简便、可行的研究和应用方法，但精子结合并内化转运外源DNA却具有非常复杂的机制。

Making transgenic animal by SMGT is a very simple and feasible method for research and application , but combining and inner - transferring of outer origin DNA has very complicated mechanism .

对BAC进行了定位外源基因插入，为转染细胞和制备转基因动物生物反应器提供了一个有效的载体。

Modification of BAG provided a effective vector for transfection of cells and preparation of transgenic animal bioreactors .

转基因动物在microRNA研究中的应用

The Application of Transgenic Animals in MicroRNA Research

利用转基因动物模型研究胰岛素样生长因子-I（IGF-I）在中枢神经系统中的生物学作用

The Research of Insulin-like Growth Factor-I 's Biological Role in Central Nerve System by Transgenic Animal Model

目的：构建含杂合增强子UI的人衰变加速因子（DAF）重组基因的表达载体，应用于转基因动物克服异种器官移植排斥反应的研究。

Objective : To construct the recombinant human decay accelerating factor gene using UI enhancer for high-performance expression in xenotransplantation .

通过G418筛选细胞，为本实验室利用核移植技术生产转基因动物提供细胞来源。

So the cells can be used as sources to create transgenic animal by nuclear transplant techniques .

用于细胞基因调控研究的RNA干涉技术和基因诱导表达方法，现在移植到了转基因动物中，并获得了理想结果。

RNA interference methods and gene inducible expression techniques to use on study of gene regulations in cells are transferred to making transgenic animals , and the ideal research results are gained .

脊椎动物P450s超家族在转基因动物中的研究进展

Advances in the Vertebrate P450s Superfamily in Transgenic Animals

FISH能直观地反映外源基因在转基因动物染色体上的整合状态，该方法可对转基因动物及基因转移研究中的外源基因整合后进行染色体定位检测。

Human β E globin gene was integrated into the chromosomes of transgenic mice in a random pattern and the results demonstrated that FISH can be used to investigate the integration site of foreign genes in transgenic mice .

鉴于此，本研究拟通过RT-PCR的方法克隆该基因，为利用转基因动物乳腺生产人骨形成蛋白创造条件。

Therefore , we tried to clone the complementary DNA of hBMP-3 gene by RT-PCR , for the purpose of producing human morphogenetic protein in the mammary glands of transgenic animals .

结论：建立了能实现种系传递的C57BL/6J小鼠胚胎干细胞系，该系的ES细胞可用于今后制备转基因动物和基因敲除动物。

Conclussion : We have obtained C57BL / 6J ES cell lines with a potential germ-line contribution , which can be applied to generate transgenic and gene knock-out mice .

卵母细胞胞质内单精子注射（ICSI）作为新型的体外受精技术，可用于研究受精机理、治疗男性不育和生产转基因动物。

Intracytoplasmic sperm injection ( ICSI ) is an in vitro fertilization technique valuable for studying fertilization , treating human infertility and producing transgenic animals .

缺氧诱导因子1（HIF-1）是在肝细胞和转基因动物上进行促红细胞生成素（EPO）基因表达研究时发现的一种转录因子。

Hypoxia inducible factor-1 ( HIF-1 ) was a transcription factor which found in the liver cells and transgenic animals during the erythropoietin ( EPO ) gene expression study .

转基因动物乳腺生物反应器研究进展鸡的原始生殖细胞（primordialgermcells，PGCs）是鸡各级生殖母细胞和成熟配子共同的祖先，最终发育分化成卵子或者精子。

Progress in Mammary Gland Bioreactor of Transgenic Animals Chicken primordial germ cells ( PGCs ) were the ancestor of all level germ cell and transgenic animals have become important tools for biological research .

胞质内精子注射（ICSI）技术能够利用精子作为载体生产转基因动物，以及使用无运动能力的冻干精子。

ICSI technique can be used to extend the sperm vector for transgenic goat production and to use freeze-dried sperm for which spermatozoa motility is not required .

近年来在AD许多研究领域特别是遗传学和转基因动物方面取得了长足的进展，使得我们对AD有了更深的理解。

Recently , many significant advances have been achieved in a variety of respects of Alzheimer 's Disease ( AD ), especially in molecular genetics and transgenic animal models , which lead to more understanding in the pathogenesis of this degenerative disease .

研究者们试图用ES细胞建立糖尿病、老年型痴呆、帕金森氏症等遗传疾病转基因动物模型。

With the help of ES cells , scientists are trying to set up the cell and animal models of diseases such as diabetes , Alzheimer 's disease and Parkinson 's disease , produce the transgenic animals .

本研究摸索出了一种高效YAC内基因打靶的新方法，并探索了通过细胞融合的方法将YAC外源基因导入哺乳动物体细胞内的可行性，这为利用YAC进行转基因动物的生产提供了借鉴。

Explored the method of the YAC transfected into goat fibroblast cell by spheroplast fusion and proved the possibility . of this method .

目前，国外对AD病的致病机制的研究主要集中在转基因动物模型上，国内尚无通过转基因技术建立转基因AD细胞模型并使用NGF进行干预的报道。

Nowadays , the research on the pathogenesis of AD at abroad focused on the abnormal expression of APP in transgenic mouse models . There were no reports of the interference of NGF on transgenic AD cell models at home and abroad .

转基因动物富含IIX纤维，表现出对有氧练习更好的耐受。

The PGC-1 â animals with more IIX muscle fibers showed a greater capacity for aerobic exercise , they found .

最后，在确定HU引起大鼠的生精细胞凋亡的基础上，应用转基因动物模型和分子毒理学技术深入探讨了HU的睾丸毒性作用机制。

Furthermore , after verifying that HU could cause spermatogenic cells apoptosis in rats , the mechanisms of this kind of apoptosis induced by HU were studied in depth using a FasL transgenic mice model and the relevant technologies of molecular toxicology .

开展SSCs的研究对于精子发生机理研究、遗传性疾病治疗、转基因动物生产及多潜能干细胞制备具有十分重要的意义。

The research of SSCs is very important for the study of spermatogenesis , cure of certain hereditary diseases , construction of transgenic animals and generation of pluripotent stem cells .

近年来在转基因动物和正常动物上的研究发现，与PKA途径相比较，CaMKⅡ信号途径可能是β肾上腺素诱导的舒张期钙离子渗漏所导致的触发活动中的主要信号途径。

Recently several studies from transgenic mice model and normal animals showed that CaMK ⅱ emerges as a dominant signal pathway for triggered arrhythmias induced by diastolic calcium leak upon adrenergic stimulation compared with PKA .

转基因动物技术与YAC（酵母人工染色体）克隆及定位突变技术的结合可望阐述大片段基因（簇）中不同部位对发育阶段特异性表达的调控规律。

Moreover , yeast artificial chromosome ( YAC ) cloning and site-specific mutation techniques can be combined with transgenic technique to investigate the developmental gene regulation of large genes ( clusters ) with mutations in different sites .

研究裸鼠的繁殖、饲养规律，寻找廉价、饲养方便、体大、生命力强的裸鼠，以及对MPM转基因动物模型的研究，将有希望获得多种满足不同需要的理想MPM实验模型。

It is promising obtaining ideal MPM experiment model which can gratify various purpose by investigating on the breeding and grooming of nude mice that are economic , convenient for breeding , large and high survive rate , and by studying MPM transgenic animal model .

山羊胚胎干细胞（ESCs）和胚胎生殖细胞（EGCs）是生产转基因动物、克隆动物，制备乳腺生物反应器等的理想候选细胞，然而至今仍没有成功建系的报道。

Goat ESCs and embryonic germ cells ( EGCs ), which may be perfect cells for producing transgenic , coloned animal and making bioreactor , Nevertheless , these has not been any successful report in relation to any of the establishment of the serious .