分子生物学技术

利用分子生物学技术制备了重组质粒、体外转录RNA，通过鉴定后建立了相应的重组质粒DNA阳性参比品、体外转录RNA阳性参比品。

Recombinant plasmid and in vitro transcription RNA were prepared and identified through molecular biology techniques .

分子生物学技术在HPV分型方面的应用进展

Application progress of molecular biology techniques in detection of human papillomavirus genotypes

90年代以来，分子生物学技术广泛应用于物种的遗传变异和分类，直接从DNA水平反映基因本身，具有优越性。

Since 1990 , molecular biological methods have been popularly used in genetic variation and classification .

反义RNA技术是用反义RNA链去抑制靶基因的活性，从而达到对目的基因调控的一项分子生物学技术。

Antisense RNA molecules deactivates target gene by binding to the sense strand .

分子生物学技术：包括多聚酶链式反应（PCR）、随机扩增DNA法（RAPD）；

Molecular bilogical technique : including polymerase chain reaction ( PCR ) and random amplified polymorphic DNA ( RAPD );

目前，常用的检测以血清学技术的ELISA为主、分子生物学技术的RTPCR为主。

At present , serological detection is mainly based on ELISA and molecular biology technique on RT-PCR .

分子生物学技术的飞速发展及其应用，为人们在DNA水平上研究猪重要经济性状的遗传机制奠定了坚实的基础。

The rapid development and application of molecular biotechnology provides a strong support to study the inherited mechanism of pig important economical traits on DNA level .

rDNA分子生物学技术及其在瘤胃微生物应用的研究进展

The Review on the Application of rDNA Molecular Biology Techniques in Rumen Microbes

虽然孕妇血中胎儿核酸物质的来源目前仍不清楚，应用相对简单的分子生物学技术已经可以进行胎儿DNA的高灵敏度、高特异性的检测。

Although the origin of fetal nucleic in maternal circulation is unknown at present , researchers now can detect fetal nucleic acids sensitively and specifically through simple molecular technology .

本文综述了PV的几种常见的实验室诊断方法及分子生物学技术在诊断PV方面的应用。

The article reviewed the laboratory testing and the application of molecule biologic techniques in the diagnosis of PV .

现代分子生物学技术也被用于一些软骨鱼类的基因组研究，在重复DNA序列、随体DNA家族和微卫星位点等方面均取得一些成果。

The genomes of several chondrichthian fish have also been studied by up-to-date molecular techniques , especially as regards the repetitive DNA sequences , satellite DNA family and microsatellite loci .

用分子生物学技术鉴定新生儿脐血ABO疑难血型

The Molecular Method for the ABO Blood Group Typing for the Neonate Cord Blood Samples

农杆菌介导的T-DNA标签法是近年发展的一种有效的分子生物学技术。

Agrobacterium-mediated T-DNA tagging is an effective molecular technology in recent years .

16Srdna分子生物学技术在除磷菌种群结构分析中的应用

Application of Molecular Biotechnology about 16S rDNA in Analysis of Community Structure of Phosphorus Removal Organism

本论文利用现代分子生物学技术，对五种大豆低聚糖进行了QTL定位，通过分子标记辅助选择，加快大豆低聚糖专用加工品种选育进程。

The five types of soy oligosaccharides were mapped on QTL in this paper by modern molecular biology technology .

聚合酶链式反应（POLYMERASECHAINREACTION，PCR）是最常用的分子生物学技术之一，通过变性、退火和延伸的循环来完成核酸分子的大量扩增。

Polymerase chain reaction ( PCR ) is one of the common techniques in molecular biology , which can amplify nucleic acids through the cycle of denaturation , annealing and extension .

目的用分子生物学技术对新生儿脐血ABO疑难血型鉴定。

Objective To resolve the ambiguous determination for ABO blood group in neonate cord blood by molecular method .

使用分子生物学技术，研究人员分离出损伤肺部的M2蛋白的片段。

Using molecular biology techniques , scientists isolated the segment of the M2 protein responsible for the damage to the lung protein .

分子生物学技术的成熟使人们从设计改造功能蛋白转向设计基因，并尝试进行基因治疗和DNA免疫。

The mature of molecular biotechnology made it possible for people to design gene instead of remaking function protein , and to use the recombinant DNA techniques for gene therapy and DNA immunization therapy .

并采用分子生物学技术聚合酶链式反应&变性梯度凝胶电泳（POLYMERASECHAINREACTION-denaturinggradientgeleleetrophoresis，PCR-DGGE）对污泥微生物群落的演替展开研究。

The microbial community structures and succession were studied by polymerase chain reaction-denaturing gradient gel eleetrophoresis ( PCR-DGGE ) .

将ES细胞分离与克隆技术和分子生物学技术相结合就可将新的遗传物质导入家畜的生殖腺细胞。

The new genetic material can introduce into germ cell in farm animal by the combination of embryonic stem culture with molecular biological techniques .

目的：利用分子生物学技术克隆大鼠睫状神经生长因子（CNTF）基因片段，构建腺相关病毒表达载体，为探讨CNTF对大鼠视网膜神经节细胞的影响奠定基础。

Objective To construct the recombinant adeno-associated virus ( rAAV ) vector of CNTF gene .

应用新的分子生物学技术MK可能成为基因治疗的新靶点。

Apply new molecular biology technology midkine is a candidate molecular target for gene therapy .

介绍DNA探针、细胞计量术、基因重组法和PCR技术四种现代分子生物学技术在食品微生物快速鉴定中的应用及其原理。

Four modern molecular biology techniques are introduced : DNA probes , folw cytometry , genetic recombination and PCR technique . Their principles and application in food microorganisms ' rapid indentification are also discussed .

结论PCR-RFLP法是一种有希望能够快速、准确对马拉色菌属进行种间分类的分子生物学技术。

Conclusion PCR-RFLP is a promising molecular biological technique , which could rapidly and correctly classify Malassezia species .

分子生物学技术方法PCR-DGGE在土壤微生物多样性研究中应用广泛。

As a molecular technology , PCR-DGGE is widely used in study of soil microbial diversity .

基因打靶技术是一项新兴的分子生物学技术，是利用外源DNA与受体细胞染色体DNA上的同源序列之间发生重组，并整合在预定位点上，从而改变细胞遗传特性的方法。

Gene targeting is a rising technology in molecular biology , which is defined as the introduction of exogeneous DNA to specific site in genome by homologous recombination , and consequently change the hereditary character of the cell .

运用ELISA法检测麻疹IgM抗体、麻疹病毒分离鉴定等方法，结合血清流行病学、病毒学和分子生物学技术，从病原学上明确病因；

Using the serum epidemiology , virology and molecular biology technology to make clear what the pathogen is by detecting IgM antibody by ELISA , measles virus isolation and identification .

利用分子生物学技术，构建表达丙型肝炎病毒（HCV）包膜蛋白E2的人源单链可变区抗体（ScFv）的原核表达载体，并在大肠杆菌JM109中表达可溶性的HCVe2ScFv。

To construct expressive vector for human ScFv against E2 protein of hepatitis C virus ( HCV-E2-ScFv ), and express soluble HCV-E2-ScFv in E.

经过条带测定，样品中还存在着较多尚未被认识的微生物，这也说明使用基于非培养手段的分子生物学技术直接对酒曲样品总DNA分析有助于发现新的微生物种类。

There were still more yet to be known micro-organisms after bands determination , which also illustrated that direct analysis lof the total DNA in fermentation samples based on non-culture molecular biology techniques helped the discovery of new microbial species .