解折叠

在此基础上，我们以bis-ANS为外源荧光探针，利用荧光光谱学等方法比较详细的研究了rPIP的折叠与解折叠，并将相关结果与人胰岛素等进行了比较。

Based on this observation , we used bis-ANS as an extrinsic fluorescence probe to explore folding / unfolding of rPIP in detail and then compared the results with human insulin .

在含有巯基试剂的变性剂中，PIP产生二硫键交换从而形成一系列具有不同解折叠程度的二硫键异构体混合物。

In the presence of denaturants and thiol reagents , the native structure of PIP was disturbed and its native disulfides were shuffled to form a mixture of scrambled isomers which have different degrees of unfolding .

mRNA解折叠的链构象与相应新生肽链构象相关性的探索而当湿度小于75%大于40%范围时分子的二级结构改变以β折叠和无规卷曲的肽链构象。

The Correlation Between the Chain conformations of Unfolded mRNA and Corresponding Nascent Polypeptide R 40 % - 75 % , it can be obtained as antiparallel β - cheet and random coil conformation .

重组单链胰岛素在含有巯基试剂的变性剂中的解折叠

Unfolding of Recombinant Single-chain Insulin in Denaturants Containing Thiol Reagents

热力学研究表明，该蛋白质对压力非常敏感，极易被高压力解折叠。

Thermodynamic equilibrium studies indicate that the protein is very sensitive to pressure .

在某些情况下，蛋白质将会解折叠成散乱的形状并且失去生物功能。

Under some conditions , protein will unfold into random coil that loses biochemical function .

鲤鱼类胰岛素生长因子-Ⅰ的克隆和原核表达研究小型类胰岛素生长因子-1的制备及其体外解折叠的初步研究

Cloning and Prokaryotic Expression of Cyprinus Carpio Insulin-like Growth Factor - ⅰ Gene ; Purification and Putative in Vitro Unfolding Pathway of Mini-IGF-1