梯度离心

快速密度梯度离心法纯化质粒DNA

Purification of plasmid DNA by rapid density gradient centrifugation

方法细胞：采用密度梯度离心法，分离人脐血单个核细胞（MononuclearCells，MNCs）。分组：分为3组。

Method Human umbilical blood mononuclear cells ( MNCs ) were separated with density gradient centrifugation .

采用梯度离心方法从兔骨髓中分离培养BMSCs。

BMSCs were isolated and culture-expanded from rabbit bone marrow .

密度梯度离心法获得大鼠MSCs，传代培养并扩增。

MSCs were obtained from SD rats using the method of density gradient centrifugation culture .

结果1密度梯度离心法成功分离纯化大鼠MSCs。

Rat MSCs had been separated and purified by density gradient centrifugation successfully . 2 .

结论1.经观察细胞形态和细胞表面标志物鉴定，采用密度梯度离心结合贴壁法可在体外成功分离、培养MSCs。

MSCs can be isolated by density gradient centrifugation and adherent culture in vitro . 2 .

结果：1.体外培养MSCs的形态特征经密度梯度离心的MSCs形态比较均一。

Morphological characteristics of MSCs in vitro by density gradient centrifugation of MSCs relatively uniform shape .

结果：Percoll密度梯度离心法培养可获得均一的MSCs；

RESULTS : Homogeneous MSCs were obtained by percoll density gradient centrifugation .

结论：用密度梯度离心和贴壁培养法可以分离并培养得到bMSCs。

Conclusion : bMSCs can be obtained from bone marrow by the density gradient centrifugation method .

方法：选用54只雄性SD大鼠，密度梯度离心法分离骨髓间充质干细胞（MSCs）。

METHODS : Bone marrow MSCs were obtained by density gradient centrifugation from the tibiae of 54 male SD rats .

结论利用Percoll密度梯度离心法结合贴壁法可以获得比较纯的MSCs；

Conclusion High pured MSCs can be obtained by Percoll gradient centrifuging and adherent method .

Percoll密度梯度离心分离不同发育阶段生精细胞的方法

Separation of Mouse Spermatogenic Cells by Centrifugation in Percoll Density Gradients

Percoll梯度离心前后精子功能指标分析

Analysis of Sperm Functional Parameters before and after Percoll Gradient Centrifugation

通过密度梯度离心与红细胞裂解后联合贴壁培养法可获取较为纯化的MSCs。

MSCs were purified by the method of split red blood cell , combining gradient density centrifugation and adhesion separation .

结论联合采用密度梯度离心和贴壁筛选的方法易于分离、纯化获得纯度较高的MSCs。

Conclusion : MSCs is easy to be isolated and purified by combined using density gradient centrifugation and plastic adherence .

采用蔗糖梯度离心法分离HL-60细胞的胞膜、胞浆和胞核；

Using sucrose gradient centrifugation to separate the membrane , the cytoplasm and the nuclei of HL-60 cells .

Percoll梯度离心与精子运动轨迹图像分析

Study on Percoll gradient centrifugation and motive tracks of sperm

梯度离心法分离食管Langerhans细胞方法初探

Preliminary exploration of the method to isolate esophageal Langerhans cells with discontinuous density gradient centrifugation

脊髓灰质炎病毒（Poliovirus）的Percoll等密度梯度离心分离

Poliovirus Separation by Centrifugation in Percoll Isopycnic Gradients

所有实验组病例和对照组均采取4毫升静脉血，肝素抗凝，密度梯度离心法分离PBMCs。

PBMCs were separated from 4 ml heparinized blood by density gradient centrifugation .

Percoll密度梯度离心法分离大鼠肺Ⅱ型上皮细胞

Isolation of Rat Type ⅱ Alveolar Epithelial Cells by Discontinuous Percoll Gradient Centrifugation

采用Percoll非连续密度梯度离心法纯化大鼠乳鼠心室肌细胞

Enrichment of cardiomyocytes from neonatal rat ventricular tissues by discontinuous percoll gradient centrifugation

目的：通过分析Percoll梯度离心法处理前后精子相关功能指标，评价该法在精子体外处理中的作用。

Objectives : To evaluate the sperm functional parameters with Percoll gradient centrifugation .

研究方法：采用Percoll密度梯度离心法分离大鼠BMSCs，对所获得的细胞进行体外培养及鉴定。

Methods BMSCs were isolated by percoll solution from rat bone marrow sample , cultured and identified .

对传统的Southern杂交法进行了改进：用碱变性和酚抽提法提取重组质粒，省去氯化铯梯度离心；

The procedure of Southern hybridization has been improved as follows : the plasmid DNA which contains DNA fragments for probe was isolated by alkaline lysis without utilizing cesium chloride gradient centrifugation .

本文采用区带密度梯度离心，国内首次分离得到黄牛纯化的高密度脂蛋白（HDL）。

The bovine high density lipoprotein ( HDL ) was first isolated and purified in our country with zonal gradient ultracentrifugation .

方法采用机械分离、酶消化和不连续密度梯度离心法分离提取TIL前体细胞；

Methods TIL were obtained through mechanical separation , enzyme digestion and discontinuous density gradient centrifugation .

应用Percoll非连续密度梯度离心法富集人前列腺上皮细胞

Enrichment of Epithelial Cells from Human Benign Prostatic Hyperplasia by Discontinuous Percoll Gradient Centrifugation

Percoll等密度梯度离心法分离利什曼原虫细胞核

Isolation and purification of nuclei of Leishmania gerbilli by Percoll isopycnic density gradients centrifugation

用改良胶原酶消化技术和聚蔗糖密度梯度离心方法将大鼠胰岛从胰腺分离，按Sun等的方法用海藻酸和聚赖氨酸等予以微囊化。

Rat pancreatic islets were microencapsulated with , sodium alginate and poly-lysine , according to Sun 's method after being isolated from pancreatic tissue using modified collagenase digestion and Ficoll density gradient separation .