苯唑西林

MRSA胶乳凝集试剂盒可快速、准确地检测耐苯唑西林的CNS。

The MRSA screen test accurately and rapidly detects oxacillin resistance in CNS .

目的比较头孢西丁纸片扩散法与传统的苯唑西林纸片扩散法对耐甲氧西林葡萄球菌（MRS）检测的效率。

Objective To compare the efficiency between the cefoxitin disk diffusion test and oxacillin disk diffusion test on the detection of methicillin-resistant staphylococcus ( MRS ) .

对青霉素G，苯唑西林，红霉素的耐药性最严重，均在80%以上；

The resistant rate to penicillin G and erythromycin was worst , all over 80 % .

而EC能进一步加强ECg与苯唑西林对MRSA协同抗菌作用。

And EC can further enhance the synergic effects of ECg and Oxacillin against MRSA .

全部MRSA均对青霉素，氨苄青霉素和苯唑西林耐药；

All MRSA were resistant to penicillin and many of them to cephalosporins .

耐万古霉素金黄色葡萄球菌占3.3%（4/122）。结论：耐苯唑西林的金黄色葡萄球菌表现为多重耐药性，且对12种抗生素的耐药率明显高于苯唑西林敏感的葡萄球菌，P0.01。

Conclusion : MRSA showed multiple resistance to antibiotics , the drug-resistant rate to 12 kinds of antibiotics was statistically and significantly higher than that of MSSA .

方法：采用高盐琼脂加苯唑西林纸片方法筛选MRS，并用Kirbybauer法对702株临床分离的MRS作药敏试验。

Methods : MRS was detected by oxacillin-salt agar screening test . The susceptibility of 702 strains of MRS to antimicrobial agents was tested by Kirby-Bauer method .

进一步筛选发现，茶多酚中的两个单体ECg和EGCg能增强苯唑西林对MRSA的抗菌作用。

In further study , we found that there were two monomer which is ECg and EGCg , having the ability to enhance the antibacterial activity of oxacillin against MRSA .

苯唑西林耐药金黄色葡萄球菌（MRSA）和苯唑西林耐药凝固酶阴性葡萄球菌（MRCNS）检出率分别为80.65%和87.73%；

The detectable rates of Methicillin-resistant S.aureus ( MRSA ) and Methicillin-resistant coagulase negative Staphylococci ( MRCNS ) were 80.65 % and 87.73 % ;

耐苯唑西林的葡萄球菌（MRSA，MRCNS）表现为多重耐药性，其耐药率明显高于苯唑西林敏感的葡萄球菌；

MRSA and MRCNS showed multiple resistance to antibiotics , the drug_resistant rate of which was significantly higher than that of methicillin_sensitive Staphylococcus ( MSS ) .

用Kirby-Bauer法检测其对12种抗生素的敏感性，同时用E-test法检测苯唑西林和万古霉素的最低抑菌浓度。

The drug sensitivity tests were determined with Kirby-Bauer diffuse method and MICs of oxacillin and vancomycin were performed with E-test method .

非PBP2a型苯唑西林耐药金黄色葡萄球菌的研究

A Study on resistance of non-PBP 2a type to oxacillin of Staphylococcus aureus

结果在210株葡萄球菌中，mecA基因检测结果与苯唑西林敏感试验结果的一致率为96.2%。

Results In 210 Staphylococcus samples , the concordance of mecA gene detection and oxacillin resistance in the in vitro susceptibility test is 96.2 % .

当苯唑西林抑菌圈≤19mm时以E-test浓度梯度法测定其对青霉素敏感性。

The antibiotic susceptibility in penicillin was detected by E-test method when oxacillin anti-bacteria zone was ≤ 19mm .

结果在MH琼脂使用头孢西丁纸片检测MRSA优于高盐琼脂苯唑西林纸片扩散法，并与PCR检测mecA基因的方法高度一致。

Results Method with cefoxitin disk diffusion in MH disk in detection of MRSA is better than oxacillin salt agar disk ; and it is also highly consistent to the method detecting mecA gene by PCR .

ATB专家系统还从75株葡萄球菌中检测出59株耐甲氧西林葡萄球菌（MRS），比原药敏结果增加了4株，但经纸片法证实仍漏检了4株苯唑西林低耐药株。

Among 75 strains of Staphylococci , 59 strains were identified as methicillin-resistant Staphylococci ( MRS ) by ATB expert system , 4 strains more than original results , but failed to report 4 strains as MRS.

应用头孢西丁和苯唑西林纸片扩散法筛查耐甲氧西林的金葡菌（MRSA）；琼脂稀释法检测头孢西丁，苯唑西林和万古霉素的最低抑菌浓度（MIC）。

MRSA was screened by oxacillin and cefoxitin disk diffusion methods . Agar dilution method was used to determine the minimal inhibitory concentrations ( MICs ) of oxacillin , cefoxitin and vancomycin to isolates of staphylococcus aureus .

苯唑西林筛纸片筛选耐药率达35.7%，进一步经青霉素E-test检测，耐青霉素肺炎链球菌（PRP）检出率实际为19.0%。

The resistant rate of penicillin in oxacillin screening slip was 35.7 % . The inspect account of penicillin-resistant Streptococcus pneumoniae ( PRP ) was 19 % in the penicillin E-test .

方法用苯唑西林纸片扩散法、头孢西丁纸片扩散法、mecA基因扩增等方法检测MRSA，用随机扩增多态DNA（RAPD）法对所分离的112株MRSA进行同源性分析。

Methods Oxacillin disk diffusion , cefoxitin disk diffusion test and PCR amplification of mecA gene were used to confirm MRSA strains . A total of 112 MRSA isolates were typed by randomly amplified polymorphic DNA ( RAPD ) .

目的评价黄芩苷分别与3种β-内酰胺类抗生素（苯唑西林、氨苄西林、头孢唑啉）对20株MRSA的体外抗菌效应。

Objective : To evaluate in-vitro antibacterial activities of 3 kinds of β - lactams antibiotics ( Oxacillin , Ampicillin and Cefazolin ) alone or in combination with baicalin at various doses against 20 MRSA strains .

MecA-PCR结果显示：6株耐苯唑西林株mecA基因均阳性，而标准株ATCC25923和所有敏感株均阴性。

The mecA-PCR showed that the control strain ATCC 25923 and all oxacillin-sensitive S. aureus were mecA-negative , while 6 oxacillin-resistant strains were mecA-positive .

方法：用mecA基因检测MRSA乳胶凝集法、头孢西丁纸片扩散法和苯唑西林微量稀释法对临床分离的160株金黄色葡萄球菌进行检测并作比较。

Methods PCR amplification of the mecA gene , the MRSA-screen latex agglutination test , disk diffusion method with cefoxitin and the microdilution method of oxacillin were performed to analyze 160 clinical isolates of S. aureus .

结论本研究中金葡菌对苯唑西林的耐药率不高，而mecA-PCR技术可以作为快速检测耐苯唑西林金葡菌的有效方法。

Conclusion Oxacillin-resistance in S.aureus isolates was low , and mecA-PCR method is a good choice for rapid examination oxacillin-resistant strains .

方法用纸片扩散法检测苯唑西林和万古霉素对金黄色葡萄球菌的抑菌圈直径，用E-test法检测最低抑菌浓度（MIC）。

Methods : The susceptibility of oxacillin and vancomycin against staphylococcus aureus were performed by both disc diffusion method and E-test method simultaneously in vitro , and both the zone diameters and MICs were measured by the same operator .

如果Etest法作为参考方法，则29株菌的MIC对比中Microscan法的判别误差为苯唑西林13.0%，庆大霉素3.4%，氯林可霉素27.6%；

For comparison , if Etest was used as the reference method , the interpretation discrepancies found for microscan were : 13 % for oxacillin , 3.4 % for gentamycin , and 27.6 % for clindamycin .

与苯唑西林琼脂平板筛选法比较，头孢西丁纸片扩散法检测MRSA的敏感性和特异性均为100%，而苯唑西林纸片扩散法其敏感性和特异性分别为93%（14/15）和91%（20/22）；

Compared with the results of the oxacillin agar-plate screen test , the percentage of sensitivity ( % ) / specificity ( % ) were as fallows : the cefoxitin disk diffusion test were 100 / 100 on detection of MRSA and MRCNS ;

药敏结果显示青霉素不敏感肺炎链球菌、耐氨苄西林流感嗜血杆菌、耐苯唑西林金黄色葡萄球菌和产ESBL肺炎克雷伯菌、大肠埃希菌的比率分别为55.0%，16.5%，41.2%，42.6%和4.5%。

Antibiotic susceptibility tests showed that rates of penicillin-non-susceptible S. pneumoniae , ampicillin resistant H. influenzae , oxacillin-resistant S. aureus and ESBL-positive K. pneumoniae and E. coli were 55.0 % , 16.5 % , 41.2 % , 42.6 % and 4.5 % , respectively .

药敏结果显示97·1%的菌株产生β内酰胺酶而对青霉素G耐药，82·9%的菌株对苯唑西林敏感，MIC50和MIC90分别为0·5μg/mL和4·0μg/mL；

Drug susceptibility test showed that 97.1 % of 35 strains were β - lactamase-positive and resistant to penicillin , while 82.9 % of all strains were sensitive to oxacillin with MIC_ 50 0.5 μ g / m and MIC_ 90 4.0 μ g / mL.

苯唑西林致心前区疼痛和四肢麻木

The pain Precordialgia and numbness of the extremities induced by oxacillin

建立了流动注射化学发光法测定注射用苯唑西林钠的含量。

Determination of Related Substances of Oxacillin Sodium Capsules by HPLC ;