肾细胞

突变体p53蛋白与肾细胞癌患者预后

The prognostic value of mutation p53 protein in renal cell carcinoma

材料与方法：搜集32例经手术病理证实的肾细胞癌，观察其术前边缘部的CT征象与术后病理改变对照。

Materials and Methods : CT and pathologic findings were compared and analyzed in 32 patients with pathologically proved RCC .

镉诱导大鼠肾细胞早期凋亡及bax基因表达的实验研究

Effect of cadmium on early apoptosis of NRK cells and gene expression of bax

从重组腺病毒转染人胚肾细胞的培养液中提取及纯化病毒DNA。

To extract and purify recombined adenovirus DNA from culture medium , the medium was transferred into a dialysis bag when extensive cell death was observed .

肾细胞癌中bcl-2、p53、增殖细胞核抗原表达与细胞增殖和凋亡

Relationship between cell proliferation and apoptosis with bcl-2 , p53 , proliferating cell nuclear antigen expression in renal cell carcinoma

用图像细胞术进行细胞核DNA倍体及核形态参数测定，对肾细胞癌预后判断有应用价值。

The measurment of DNA ploidy and nuclear morphological parameters by image cytometry ( ICM ) have an important value for predicting the prognosis of renal cell carcinoma .

甲肝病毒在猴胚肾细胞和Vero细胞上的分离与适应

Isolation and Adaptation of HAV on MEK Cells and Vero Cells

肾细胞癌原癌基因mdm2产物的定量分析

Quantitative analysis on the mdm2 products in renal cell carcinoma

方法应用PCR方法检测了34例新鲜肾细胞癌标本中不同染色体上的15个微卫星位点。

Methods A PCR based microsatellite instability assay was carried out using 15 microsatellite loci located on different chromosomes . Thirty four freshly frozen samples were examined .

按Fuhrman肾细胞癌形态分级系统，G；

According to Fuhrman ( 1982 ) renal cell carcinoma morphologic stage system ; Gl ?

HIF依赖性表达的自杀基因抑制肾细胞癌生长的实验研究

HIF-dependent suicidal gene therapy system suppresses growth of renal cell carcinomas

伪狂犬病病毒Ea株诱导牛肾细胞凋亡及核基质蛋白的变化

Induction of apoptosis by pseudorabies virus EA strain in bovine kidney ( mdbk ) cells and the alteration of nuclear matrix protein

非洲绿猴肾细胞（Vero）是多种病毒的适应细胞。

Africa green monkey cell ( Vero ) is the adapt cell of some kind of virus .

通过对狂犬病毒aG株在金黄地鼠肾细胞上传代培养，获得一种新型狂犬病毒毒株，即地鼠肾细胞适应株（CaG株）[1]。

By culturing and propagating aG strain of rabies virus in primary golden hamster kidney cells ( GHKC ), a new adapted strain was selected .

快速获得大量鱼类肾细胞中期分裂相的PHA体内注射法

A PHA injection method in vivo for the rapid obtainment of large numbers of metaphase figures from kideny cells of teleosts

方法：应用抗TGF－β1多克隆抗体对43例肾细胞癌进行免疫组化研究（同时取10例尸检正常肾组织作为对照）。

Methods : Immunohistochemistry was used with anti-TGF-1 Multi-Ab to inspect the expression of TGF-1 in 43 cases of renal cell carcinoma and 10 cases of normal renal tissues as contrast .

目的：了解肾细胞癌（RCC）患者抗瘤免疫状况及TIL与肾癌愈后关系。

Purpose To get a comprehensive understand of antitumor immune state and relationship between TIL and prognosis of renal cell carcinoma ( RCC ) .

试验组脾、肝、肾细胞的拖尾率和DNA迁移距离与阳性对照组的同类细胞比较都显著要低（P<001，P<0001）。

The percentages of cells with comets and the DNA migrations of the three types cells in tested groups were low significantly ( P < 0 01 , P < 0 001 ) compared with the corresponding positive control groups .

地鼠肾细胞培养的CTN株狂犬病新疫苗研究

Study on a new rabies vaccine prepared from CTN strain virus propagated in primary hamster kidney cell culture

随着人用狂犬病疫苗的发展，细胞培养疫苗包括地鼠肾细胞疫苗、Vero细胞疫苗等代替了脑组织疫苗。

The human-used rabies vaccine is routinely prepared from virus-infected cells such as hamster kidney cells or Vero cells other than brain tissue culture .

本文评价了抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法用于检测兔肾细胞中的单纯疱疹病毒（HSV）抗原。

An evaluation on the application of avidin-biotin-peroxidase complex ( ABC ) method in the detection of herpes simplex virus ( HSV ) antigens was made in rabbit kidney cells .

其次，将上述融合蛋白表达载体转入293T人胚肾细胞，借助荧光显微镜，观察不同融合蛋白在正常氧和缺氧细胞中的表达，发现DOG融合蛋白的稳定存在依赖于缺氧环境。

Secondly , the fusion proteins expressed in 293T cells were observed by fluorescence microscopy . The result showed that expression of DOG fusion protein was induced in hypoxia .

结果：囊性肾细胞癌术前影像学检查的正确率为76.9%，手术治疗的5年生存率达92.3%，核分级和TNM分期较低，肿瘤细胞类型为透明细胞。

The 5 year disease specific survival rate was 92.3 % after operation . The CRCC was of low nuclear grade and TNM stage composing of clear cells .

猪内源性逆转录病毒体外感染人胚肾细胞系HEK-293的初步研究

Preliminary study on human embryonic kidney cell line HEK-293 after porcine endogenous retrovirus infection

人白细胞介素2、β干扰素基因逆转录病毒载体的构建和在人肾细胞癌及其TIL中的表达

Construction of human interleukin-2 and human interferon - β gene retroviral vectors and its expression in the gene-modified renal cell carcinoma cells and its tumor infiltrating lymphocytes

方法：利用原位杂交技术，检测41例肾细胞癌和11例癌旁正常肾组织中端粒酶TERT、P21、P27蛋白的表达。

Methods In41 renal cell carcinoma and11 renal tissues adjacent to cancer , the telomerase TERT , P21 protein , P27 protein were measured by in situ hybridization .

为了探讨评估肾细胞癌（RCC）预后指标，应用免疫组化法研究60例RCC中细胞粘附分子CD15和Bcl-2蛋白表达及其相关关系。

To study the prognostic predictor of renal cell carcinoma ( RCC ), the relationship between CD15 and bcl 2 protein expression in 60 cases of RCC was studied by immunohistochemical method .

结果在透明细胞肾细胞癌中c-kit呈阴性，26例乳头状肾细胞癌和20例嫌色细胞肾细胞癌标本均呈c-kit强阳性表达；

Results c-kit showed negative in clear RCCs . However , it was specifically expressed in all cases of the papillary RCC and chromophobe cell type carcinomas .

结论肾细胞癌中存在由VHL基因突变和LOH导致的VHL双等位基因失活现象，VHL双等位基因失活发生率为37%。

Conclusion Biallelic inactivation of VHL gene occurs in RCC due to VHL mutation and LOH , and its frequency rate is37 % .

载体构建成功后，用磷酸钙沉淀法将三质粒共转染来源于人胚肾细胞系的包装细胞&293T，12h后在荧光显微镜下观察绿色荧光的表达情况。

Human embryonic kideny 293T cells were co-transfected with the three plasmids by calcium phosphate DNA precipitation and the expression of GFP was examined under fluorescent microscope 12 hours after transfection .