肝活检

肝活检对无症状HBV携带者的临床意义

Clinical Significance of Liver Biopsy for Asymptomatic HBV Carriers

免疫酶（直接法）染色对95例肝活检组织内HBsAg的初步研究

Preliminary Investigation of Immunoperoxidase Staining ( Direct Method ) of HBsAg in 95 Liver Biopsy Specimens

B组中6例分别于治疗前后行肝活检术。

Six cases of group B were taken liver biopsy twice .

目的建立一种肝活检组织中HBV共价闭合环状DNA的定量检测方法。

Objective To establish a method for detecting HBV cccDNA in hepatocytes of chronic hepatitis B patients .

间接免疫荧光法检测肝活检组织中HCV抗原

Detection of HCV antigen in liver tissue with indirect immunofluorescence technic

结论移植肝活检及活检前生化检测对于AR的诊断与鉴别诊断有重要意义。

Conclusion Biopsy of the graft and the biochemical examination before biopsy are of help in the diagnosis of AR.

方法：对112例慢性HBsAg携带者进行肝活检，了解肝脏组织学改变，同时进行B型超声波检查及血清HA、LN、Ⅳ、PⅢ水平检测。

Methods : 112 patients with chronic HBsAg carrier were undertaken liver biopsy and examined by ultrasonic B , HA .. LN .

目的研究自身免疫性肝炎（autoimmunehepatitis，AIH）的临床表现、肝活检病理学特征。

Objective To determine clinical manifestation and pathological changes in autoimmune hepatitis ( AIH ) by liver biopsy .

目的：探讨细针穿刺肝活检组织行端粒酶活性检测用于肝细胞癌（HCC）诊断的可行性。

Objective : To explore the feasibility of detection of telomerase activity in fine-needle biopsy tissues of hepatocellular carcinoma ( HCC ) .

以光镜观察了5例胃肠瘘行全肠外营养（totalParenteralnutrition，TPN）支持治疗之肝活检标本。

The liver biopsy specimens were obtained from 5 cases with total parenteral nutrition ( TPN ) for gastrointestinal fistula and examined by light microscope .

方法把通常的肝活检病理诊断和本课题组所采用的3名专家独立读片的方法用Kappa分析进行检验和比较。

Methods Kappa analysis was used to test and compare the two methods of pathological diagnosis in liver biopsies .

随机采取100例乙型肝炎肝硬化患者，行肝活检术取肝组织，采用免疫组织化学和原位分子杂交法，检测其中HBcAg和HBVdna。

In these cases , the biopsy of liver were performed and HBcAg and HBV DNA were detected by immuno-cytochemistry and in situ molecule hybridization technique .

分析AR组、保存再灌注损伤（PRI）组移植肝活检前患者生化检测值。

The biochemical changes of AR group and preservation-reperfusion injury ( PRI ) group were examined before biopsy of the graft .

方法：观察292例经肝活检证实的各类肝炎患者血清PA、ChE水平和血清肝纤维化指标的变化，并将其与肝组织活检的炎症分级和纤维化分期进行相关性分析。

Methods Detection of serum PA , ChE and serum fibrosis markers together with liver biopsy was performed in 292 patients with hepatitis .

方法：对82例慢性肝炎肝活检组织作HE染色及网状纤维嗜银染色，分别判断慢性肝炎炎症活动度及纤维化程度，同时检测患者血清HA、LN、Ⅳ。

Method : Biopsy was performed in 82 cases with chronic hepatitis and HE stain and reticular fibre argyrophil stain were done to determine the hepatits activity and fibrosis degree .

材料和方法：对肾移植患者检测出乙肝病毒表面抗原（HBsAg）阳性者进行肝活检，由此确定病毒复制证据。

PATIENTS AND METHODS : Renal transplant patients positive for HBV surface antigen ( HBsAg ) and submitted to a liver biopsy because of evidence of viral replication were included .

应用地衣红、醛复红染色、免疫荧光和双桥PAP法，对显示石蜡切片肝活检标本中的HBsAg进行了比较。

Staining methods of orcein , aldehyde fuchsin , immunofluorescence and double bridge PAP technique for identifying HBsAg have been compared on paraffin sections of hepatic biopsy materials .

方法250例乙肝患者肝活检标本用VEGF单克隆抗体进行免疫组织化学（S－P法）染色。结果VEGF在肝组织表达有胞浆型、膜窦型及窦内皮细胞型；

Methods Immunohistochemical staining was performed with VEGF monoclonal antibody in 250 biopsies of HB . Results The types of VEGF expression in liver tissues were of plasma type , sinusoidal membrane type and sinusoidal endothelial cell type .

目的探讨肝活检组织学对转氨酶持续正常6个月以上的慢性乙型肝炎（CHB）感染的临床意义。

Objective The histological features of chronic hepatitis B virus ( HBV ) infection with persistently normal serum transaminases has not been will studied .

用树鼩CDNA芯片检测实验第30、60、90周肝活检组织、肝癌组织及其癌旁组织中各基因的表达情况，并用实时逆转录聚合酶链反应（realtimeRT-PCR）法验证cDNA芯片结果。

Tree shrew-specific cDNA microarray was applied for detecting the differential expression of corresponding genes in each group at different time points during the experiment , and real time RT PCR was applied to verify the results of the cDNA microarray .

结果应用拉米夫定+HBIG预防的71例中，有2例再感染，血清HBSAg为阳性，肝活检免疫组织化学检测有HBSAg表达，其中1例血清HBVdna阳性，另1例经治疗后HBSAg又转阴。

Result 2 of 71 cases given lamivudine + HBIG developed reinfection , which serum HBSAg was positive and liver biopsy immunohistochemistry showed HBSAg phenotype . The serum HBV DNA was positive in one of them .

结果应用拉米夫定的2例病人，有1例发生再感染，其血清HBsAg、抗Hbe、抗HBc和HBVdna均呈阳性，肝活检免疫组织化学检测有HBsAg表达。

Results One of the 2 cases given lamivudine developed reinfection , whose serum anti-HBsAg , anti-HBe , anti-HBc , HBV DNA were positive and liver specimen immunohistochemical study showed HBsAg phenotype .

结论：腹腔镜检查和/或肝活检是DJS诊断和鉴别诊断的重要方法。

Conclusion : Laparoscopy and / or hepatic biopsy are the very important methods for diagnosis and differential diagnosis of DJS .

方法：以随机引物法标记人perforincDNA探针，对慢性丙肝患者肝活检标本进行原位杂交，观察肝局部浸润炎性细胞perforinmRNA的表达及分布，分析他们与肝损伤之间的关系。

METHODS : The expression and distribution of perforin mRNA in the local infiltrated cells were detected by in situ hybridization using a dig labeled perforin cDNA probe in liver biopsy tissues .

目的探讨肝脏病变程度及维生素K1对慢性肾功能衰竭病人肝活检术后出血的影响。

Objective To investigate the influence of liver lesions and vitamin K1 on bleeding after liver biopsy in patients with chronic renal failure and hepatitis virus infection .

在经肝活检诊断的慢性乙型活动性肝炎，两者的检出率在病情增重期显著高于缓解期，并高于肝组织正常的无症状HBV携带者。

In biopsy-proven chronic active hepatitis ( CAH ) B , prevalence of both tests were significantly higher at exacerbation than at remission of the disease , and so were in CAH than in chronic asymptomatic HBV carrier with normal histology .

结论NAFLD是近年来较常见的慢性肝病，肝活检组织学评价是确诊的金标准。

Conclusion NAFLD is a common form of chronic liver disease in recent years and histology of liver biopsy , remains the gold standard in evaluation of NAFLD .

Menghini法肝活检的操作问题

Some Problems on Manipulation of Menghini Liver Biopsy

共有49例病人作二次肝活检，其中A组25例，B组24例。治疗52周后，A组能显著改善病理组织学SSS计分系统肝纤维化程度及肝脏炎症程度；

49 patients had liver biopsy twice , 25 patients in group A and 24 in group B. At the end of therapy , significant improvement of the degree of liver fibrosis and inflammatory of SSS were noted in group A.

方法：对210例乙肝患者肝活检组织及40例CCl4实验大鼠不同时期肝组织，用VEGFmAb进行免疫组织化学（免疫组化）染色；

Methods : Immunohistochemical staining was performed by means of VEGF mAb on the liver tissues in 210 patients with HB and on the liver tissues in different stages induced by CCl4 in 40 rats .