组织分离法

实验发现孢子分离法和组织分离法均能分离得到冬虫夏草母种，但组织分离法操作简单，成功率高，孢子分离法操作复杂，难度较大。

The result showed that parent species of C. sinensis could be isolated by spore separation and tissue isolation methods . The tissue isolation was more simple and had higher success rate .

结果如下：1、首次采用组织分离法从杜仲的根、茎、叶中分离到80株内生真菌。

The results are as the follows : 80 strains of endophytic fungi were separated from roots , stems , and leaves of E. ulmoides by tissue isolation method for the first time .

病组织分离法是分离大豆疫霉根腐病菌的有效方法，在选择性培养基上，采用此方法分离到病原菌的成功率达86.7%。

The pathogen could be successfully isolated from the diseased tissue of soybean plants on selective media and the percentage of isolation reached 86.7 % .

实验中分别采用了组织分离法、孢子分离法和菌根分离法；（3）培养条件的选择。

The tissue separation method , spore separation method and mycorrhizal separation method were used in the experiment ;( 3 ) The selection of conditions of cultivation .

本试验采用组织分离法，分离筛选出对黄瓜灰霉病和靶斑病具有拮抗作用的菌株，并对其培养液的抑菌活性、菌株特性和防治效果等进行了研究。

This study was carried out with tissue separation method , in order to screen the strains those have inhibitory effects on the tomato grey mold and cucumber target spot .

用组织分离法[1]从陕北苹果的6种常见真菌病害的病组织中分离出了9种病原菌，并对其进行鉴定和病原学特性研究。

11 species of the pathogen fungus were selected through isolating and purifying with the organization parting method from 6 kinds of common sickness apple tissue in the North Shaanxi .

本文介绍了食用菌组织分离法制作母种的一些关键问题及易疏忽的细节，以及母种扩接技术。

This paper introduces keys to mother strain of edible mushroom through tissue separation , some details easily to be neglected , and the technique of expanded graft of mother strain .

方法组织分离法和菌丝尖端纯化法得到主要病原菌，通过测量不同营养条件下菌落生长直径研究其生物学特性，平皿菌丝生长抑制法进行杀菌剂的生物测定。

Methods The main pathogen can be gained by tissue isolation and mycelium cusp purification , the biological characteristics can be studied by measuring the growth diameter under different nutrient conditions and seeded the hyphae disk at the centre of agar plate containing chemicals to do fungicide bioassay .

该菌适宜的分离方法为常规组织块分离法。

General tissue separation was the most suitable method for the fungus .

本实验采用肺组织机械分离法体外培养大鼠肺内皮细胞，将哮喘病理血清与内皮细胞共同孵育，用于细胞粘附的体外研究。

In this study , endothelial cells were obtained from rats by mechanical isolation of lung tissues and in vitro culture on glass .

方法组织培养病毒分离法。

Method To use method of tissue cultivation and virus isolation .

用组织捣碎的固液分离法从高羊茅交战Ⅱ植株分离得到6株白色菌株，通过组织印迹以及孢子灭活试验证明所分离的菌株为内生的。

By tissue surface-sterilized , 6 strains endophytes isolated from the tissues of tall fescue .

目的探讨喉鳞癌细胞体外原代培养过程中癌组织块的适宜分离法。

Objective To find proper method for dissociation of carcinoma cells from laryngeal squamous carcinoma tissue in primary cell culture .