神经丝蛋白

采用石蜡切片SP免疫组化方法检测两组标本中的神经丝蛋白及C-Kit的表达。

By using immunohistochemical SP method , neurofilament protein and C-Kit expression were detected . 3 .

醋酸铅对ST-8814细胞神经丝蛋白、微管结合蛋白mRNA水平的影响

Effect of lead acetate on mRNA expression of neurofilament protein and microtubule associated protein in ST-8814 cell line

研究表明：AD脑神经丝蛋白的糖基化修饰水平降低，伴随着磷酸化修饰水平增加，其机制目前尚不清楚。

The study demonstrated that O-Glycosylation of neurofilaments decreased and phosphorylation increased in AD brains .

目的：观察不同胎龄胎儿脊髓神经丝蛋白（NF）阳性神经元的形态、分布和发育变化，为脊髓-脊髓移植选择适宜的胎龄提供形态学依据。

Objective : To observe the shape and distribution of NF-like neurons in human fetal spinal cord .

Alzheimer病及老年脑神经丝蛋白磷酸化异常

An Abnormal Phosphorylation of Neurofilament Proteins in Brains or Alzheimer ′ s Disease Patients and Aged Subjects

方法建立体外培养小鼠耳蜗螺旋神经节细胞的方法，并进行神经丝蛋白（NF）免疫组化染色鉴定及受体酪氨酸激酶C（TrKC）免疫组化染色。

Methods The SGCs were cultured and purged in vitro . SGCs were identified by neurofilament ( NF ) and receptor tyrosine kinase C ( TrKC ) immunocytochemical staining .

免疫组化表明hMSCs在大鼠脑内表达人神经元特异性烯醇化酶（NSE）、神经丝蛋白（NF）和胶质纤维酸性蛋白（GFAP）；

Immunohistochemical staining showed that hMSCs expressed human neuron specific enolase ( NSE ), neurofilament ( NF ) and glial fibrillary acid protein ( GFAP ) .

以免疫组化方法检测神经元特异性烯醇化酶（NSE）、神经丝蛋白（NF-M）、巢蛋白（nestin）、胶质纤维酸性蛋白（GFAP）的表达。

Neuron-specific enolase ( NSE ), neurofilament ( NF ), nestin , glial fibrillary acidic protein ( GFAP ) were detected by immunocytochemistry .

目的：从S-100蛋白和神经丝蛋白（neurofilament，NF）的不同时相分布，探讨益气化瘀方促进神经再生的机理。

Objective : To study the mechanism of the YI QI HUA YU recipe accelerate nerve regeneration by distribution of S-100 protein and neurofilament ( NF ) in different phase .

免疫组化染色选用的抗体分别为抗：①神经丝蛋白（NF-200），S-100蛋白。

The antibodies , which were used for the immunohistochemical studies , were produced against ( 1 ) NF-200 or S-100 protein ;

神经丝蛋白（NFP）阳性细胞和纤维在所检测的各个发育阶段均有表达，P53和P物质在各期胚胎视网膜均未检出阳性细胞。

The positive cells and fibers of neurofilament protein ( NFP ) appeared at all the stages of retinas but that of P53 protein ( P53 ) and Substance P ( SP ) were not found in all embryos .

结果：原代培养脊髓神经元中，神经丝蛋白的NF-H的表达，随细胞发育的逐渐成熟呈日趋上升趋势。

Results ; The expression of NF-H in primary cultured neurons of spinal cord increases correspondingly companied by cultured days .

结果：嗅球神经元可以在体外培养成功，且对神经元有特异性的标志蛋白抗神经丝蛋白（NFP）呈阳性反应。

The immunoreactivity of neurofilament pro ˉ tein ( NFP )( a specific marker protein for neurons ) was detected in the cultured neurons .

原代培养脊髓神经元神经丝蛋白NF-H表达与免疫电镜观察

Study on Expression of Neurofilament-H ( NF-H ) in Primary Cultured Neurons of Spinal Cord and Its Distribution under Immuno-electron Microscope

做神经元特异性烯醇化酶、角质纤维酸性蛋白、神经丝蛋白免疫组化染色.结果：在加入成骨细胞分化培养液后7和14d钙钴法碱性磷酸酶染色阳性细胞百分率分别为11.5%和37.5%。

RESULTS : At the 7th and 14th day after osteoblastic induction , 11.5 % and 37.5 % of the cells stained with modified Gomori were positive , respectively .

诱导后5h，12h，1d，3d，7d在倒置显微镜下观察细胞形态，免疫组化鉴定神经丝蛋白、胶质纤维酸性蛋白的表达。

At 5 and 12 hours , 1 , 3 and 7 days after induction , cellular form was observed under invert microscope , the expressions of neurofilament protein and glial fibriliary acidic protein were identified with immunohistochemistry .

用参芪液诱导MSC分化为神经元样细胞，经免疫组化鉴定其神经元烯醇化酶（NSE）、神经丝蛋白（NF）、胶质纤维酸性蛋白（GFAP）的表达。

After MSC was induced to differentiate into neuron-like cells with Shenqi Liquid , expression of neuron-specific enolase ( NSE ), neurofilament ( NF ), and glial fibrillary acidic protein ( GFAP ) were detected by immunocytochemistry , respectively .

铅对仔鼠脑Brn-3a蛋白及神经丝蛋白表达影响

Effects of prenatal lead exposure on expression level of Brn-3a protein and neurofilament proteins in offspring rat brain

方法：动用Nissl染色显示小脑皮质神经元，免疫组织化学方法显示胶质纤维酸性蛋白免疫阳性（GFAP-IR）星形胶质细胞和神经丝蛋白免疫阳性（NF&IR）结构。

Method : Nissl staining was applied to exhibit cortical neurons ; immunohistochemical method was used to display GFAP-immunoreactive ( GFAP-IR ) astro-cytes and neurofilament-immunoreactive ( NF-IR ) neurons .

结果：在海马结构内并不是所有的神经元均可被神经丝蛋白所标记。

Results : NF protein in the hippocampus only labeled some neurons .

人胚胎视网膜内神经丝蛋白的出现和分布

Occurrence and distribution of neurofilament protein in the retina of human embryos

结论：并非海马结构所有神经元内或同一神经元的不同部位均存在神经丝蛋白。

Conclusion : NF protein only exists in some neurons or parts of a neuron .

方法采用免疫组织化学显示10例正常人心脏传导系统中的神经丝蛋白。

Method The neurofilament in human cardiac conduction system of10 normal cases was demonstrated immunohistochemically .

帕金森病大鼠模型额叶皮质内突触素与神经丝蛋白的表达变化

Expression changes of synaptophysin and neurofilament protein in frontal cortex of rat model of Parkinson disease

实验性头颅侧向旋转脑弥漫轴索损伤神经丝蛋白亚单位含量变化

The content change of neurofilament protein subunit in experimental brain diffuse axonal injury by lateral head rotation

早期环境干预对脑损伤仔鼠脑神经丝蛋白表达的影响

Effect of Early Environment Intervention on Expression of Neurofilament Protein in Brain of Filial Rats with Brain Injury

大鼠生长及衰老过程中学习记忆能力与皮层神经丝蛋白表达关系

The Relation of Study Memory Function and Cortex Neurofilament Protein Expression in Growth and Senescence Course of Rats

他们认为可能是由于氯丙烯的直接作用和代谢产物的作用，使神经丝蛋白发生交联。

They think may be due to the direct effect allyl chloride and metabolites , allowing nerve silk protein crosslinking .

采用降钙素基因相关的缩氨酸和神经丝蛋白双重免疫细胞化学染色法鉴定并测定神经元的纯度。

Immunocytochemistry was performed by double staining with anti-calcitonin gene-related peptide and anti-NF200 antibodies to evaluate the purification rate of DRG neurons .

结果在心传导系统中可见到大量神经丝蛋白免疫阳性神经纤维，主要位于血管周围和结细胞间；

Results There were numerous neurofilament positive fibers in cardiac conduction system , which mainly located surrounding blood vessels or between nodal cells .