碳二亚胺

采用碳二亚胺法制备诺氟沙星与牛血清白蛋白（BSA）的结合物，作为诺氟沙星酶联免疫用的免疫原。

The antigenic conjugate of norfloxacin and bovine serum albumin ( BSA ) was prepared by carbodiimide method .

分别用碳二亚胺法和混合酸酐法制备诺氟沙星与卵清蛋白（OA）的结合物，作为诺氟沙星酶联免疫测定用的包被抗原。

The conjugates of norfloxacin-ovalbumin ( OA ) used for coating antigen were synthesized by carbodiimide method and mixed anhydride method , respectively .

用琥珀酸酐－碳二亚胺法和氯磺化法合成了毕兹（Bz）的两种人工抗原Bz＿HS＿protein和Bz＿SO2＿protein。

Two artificial antigens Bz_HS_protein and Bz_SO 2_protein have been synthesized by the succinic anhydride_ carbodiimide method and the sulfonyl chloride method separately .

然后通过碳二亚胺法将半抗原与载体蛋白偶联制备人工抗原，采用紫外扫描及SDS-PAGE鉴定。

Then , antigen was prepared by conjugation of hapten with carrier protein by carbodiimide ( EDPC ) method and was identified by UV spectroscopy and SDS-PAGE .

方法以甲壳糖为载体，碳二亚胺（EDC）为交联剂，与分离纯化的ACE混合，使ACE固定化；

Methods Isolated and purified ACE was mixed with vector , chitosan , and crosslinking agent , carbodiimide ( EDC ), and ACE was immobilized .

本研究分别以牛血清白蛋白（BSA）、卵清蛋白（OVA）为蛋白质载体，采用碳二亚胺法（CDI）将半抗原青霉素钠（PEN）分别与BSA、OVA偶联，合成了完全抗原PEN-EDC-BSA和PEN-EDC-OVA。

In this study penicillin hapten was respectively coupled with BSA and OVA to synthesize PEN-EDC-BSA and PEN-EDC-OVA complete antigen applying CDI .

用碳二亚胺（EDCI）作为缩合剂，将光敏剂血卟啉与单克隆抗体Herceptin偶联，对HP-Herceptin偶联物进行透析、凝胶过滤纯化。

EDCI was performed to conjugate monoclonal antibody Herceptin to photosensitizer - hematoporphyrin .

传统的肽类合成多采用液相化学合成，常用的包括碳二亚胺（DCC）法，混合酸酐法、酰氯法、叠氮物法等等，为多肽的合成提供了多种选择。

Peptides are traditionally synthesized in liquid phase , such methods are DCC , mixed anhydrides , acyl chloride and azide etc. , which provide more choices for peptides synthesis .

苯甲酸和苯酚在二环己基碳二亚胺（DCC）存在下，用4-二甲氨基吡啶（DMAP）作催化剂合成苯甲酸苯酯，反应条件温和，时间短，后处理容易，产率高。

Phenylbenzoate is synthesized with benzoic acid and phenol under 4 dimethylpyridine ( DMAP ) and dicyclohexylcarbodiimide ( DCC ) at room temperature . The yield is 89 %

方法一、聚苯乙烯磁性微球固化抗体采用碳二亚胺交联法，将TSH抗体和羊抗兔IgG抗体固化在聚苯乙烯磁性微球上，制备免疫磁珠，建立稳定、高效的免疫磁性微球的固化方法。

The carbon diimine crosslinking method is used to make the TSH antiboby and goat anti-rabbit IgG fixed on the magnetic polystyrene microspheres for the preparation of immunomagnetic beads , so a stable and efficient curing method for the immune magnetic microspheres is established .

叶绿体的9氨基吖啶（9AA）荧光猝灭减弱，但加二环己基碳二亚胺（DCCD）可以部分恢复9AA荧光猝灭。

Proton uptake of chloroplasts and the fluorescence quenching of 9_aminoacridine ( 9_AA ) in thylakoid membrane decreased after the thermal pretreatment , but addition of dicyclohexylcarbodiimide ( DCCD ) could partially restore the fluorescence quenching of 9_AA .

方法T-2毒素与琥珀酸酐在吡啶参与下于蒸汽浴中反应，生成T-2-半琥珀酸酐后，再与小牛血清白蛋白经碳二亚胺偶联结合生成T-2毒素抗原。

Methods T 2 toxin in pyridine reacted with succinic anhydride in a steam bath and converted to T 2HS . At the presence of EDPC , T 2HS conjugated with BSA , then BSA T 2HS was prepared .

方法采用碳二亚胺法将14型PNCPS与破伤风类毒素（TT）结合，将结合物免疫NIH小鼠，用ELISA法检测小鼠血清中抗14型PNCPS的IgG抗体滴度。

Methods 14 TT conjugates were prepared by carbodiimide mediated coupling of PNCPS with tetanus toxoid ( TT ) . Female NIH mice were immunized with conjugates or pure PNCPS type 14 , and the PNCPS antibodies in the sera of animals were detected by ELISA .

利用双功能偶联剂1-（3-二甲基-氨丙基）-3-乙基碳二亚胺（EDC）将叶酸通过叶酸活性酯的方式与BSA-DOX偶联。

Then using the bifunctional coupling agent 1 - ( 3 - dimethyl - aminopropyl ) - 3 - ethyl carbodiimide ( EDC ), folic acid was turned into the ester , coupling with the BSA-DOX .

发现碳二亚胺与伯胺反应生成咪唑啉酮环时具有一定的选择性。

The cyclizing reaction of imidazolinones has a certain selectivity .

采用碳二亚胺法和氯甲酸异丁酯法合成恩诺沙星的人工抗原，结合比为15∶1。

Using carbodiimide method and isobutylchloroformate method , two artificial antigens were synthesized .

二环己基碳二亚胺制备方法的改进

Improved method of preparation for dicyclohexylcarbodiimide

有趣的是，用含有二硫键的胱胺以碳二亚胺化学法对囊泡进行化学交联，可以得到界面交联的囊泡。

Interestingly , thus formed vesicles could be readily cross-linked at the interface using cysteamine via the carbodiimide chemistry .

其中聚合物接枝固相催化剂是新型环保型催化剂，是异氰酸酯合成碳二亚胺类催化剂的发展方向。

The polymer-grafting solid catalyst is a new catalyst beneficial to environment and is developmental direction in preparation of carbodiimide from condensation of isocyanate .

采用优化的碳二亚胺法制备诺氟沙星（NOR）人工抗原，紫外扫描和红外光谱图表明人工抗原偶联成功。

Modified EDC method was employed to synthesize the artificial antigen of norfloxacin ( NOR ), which was proved by Ultraviolet absorbance and IR spectra .

本实验采用琥珀酸酐和碳二亚胺法将沙丁胺醇偶联到牛血清白蛋白和卵清白蛋白上，成为完全抗原Sal-HS-BSA和Sal-HS-OVA。纯化后进行检测，确认沙丁胺醇已经连接到蛋白质上。

Conjugating Sal to bovine serum albumin ( BSA ) and ovi albumin ( OVA ) by succinic anhydride and carbodiimide transformed Sal , an half antigen , into complete antigen .

在异氰酸酯缩合制备2，6&二异丙基苯碳二亚胺的研究中，进行了催化剂选择实验，选择了苯酚钠为缩合反应催化剂；

In the research of preparation carbodiimides by the condensation reaction of the aromatic isocyanate , choosing phenolate as the catalyzer , the suitable weight ratio of phenolate in isocyanate is about 4 % .

高于300℃后出现的碳二亚胺可能由胺类化合物反应而生成，在400℃以后，残留物中主要成分可能由嘧嘞胺及其聚合物如嘧咙等组成。

Carbodiimide appeared in residues at temperatures higher than 300 ℃ is attributed to the decomposition of amine compounds . Above 400 ℃, chief components in the residue are melem and its polymers such as melon etc.