硫解酶

设计特异引物进行常规PCR扩增，通过测序、拼接，获得了sch蚕编码酮酯酰CoA硫解酶的完整DNA序列。

Designed the specific primers to amplify by PCR , sequencing and splicing , acquired genome DNA sequences coded 3 - ketoacyl-CoA thiolase of the sch silkworm .

同时，发现该序列只是编码蛋白SCP-x的scp基因的第一个外显子即编码酮酯酰CoA硫解酶（3-ketoacyl-CoAthiolase）的DNA序列得后半部份。

Meanwhile , found it is part of the scp gene encoded SCP-x , i.e. the tail part of the gene coded the 3 - ketoacyl-CoA thiolase .

在这些肝外组织的线粒体中，SCOT蛋白催化反应生成乙酰乙酰CoA，乙酰乙酰CoA经硫解酶转化生成乙酰辅酶A。

In the extrahepatic tissue mitochondria , SCOT catalytic reaction to generate acetyl CoA , acetyl acetyl CoA by sulfur solution enzyme into acetyl CoA .

结果是与正常家蚕编码酮酯酰CoA硫解酶的DHA序列有15个核苷酸的差异，翻译后有5个氨基酸的差异。

Result showed that there are 15 nucleoside acid differences with normal silkworm DNA sequence of 3 - ketoacyl-CoA thiolase ; mere are 5 amino acid differences after translated .

臭味假单胞菌乙酰乙酰辅酶A硫解酶的提纯及性质研究

Purification and Properties of Acetoacetyl-Coenzyme A Thiolase From Pseudomonas Putida

这表明极端嗜盐硫解酶的嗜盐特性不单来自形成的分子静电屏蔽网和疏水作用的调节，且与分子表面张力减小密切相关。

Those suggest that electrostatic screen , hydrophobic effect and surface tension all contribute to halophilic properties of thiolase .