登革病毒

登革病毒C基因突变对病毒RNA复制的影响

Effect of mutations in dengue virus C gene on viral RNA replication

登革病毒2型E蛋白基因真核表达和DNA免疫的研究

Eukaryotic Expressing and the DNA Immunization of E Protein Gene of Dengue virus 2

实时荧光PCR技术检测登革病毒的研究

Research of Real-Time Fluorescence PCR Detection on Dengue Virus

登革病毒C基因RNA二级结构及编码蛋白在病毒复制中的作用

Roles of RNA Secondary Structure in Dengue Virus C Gene and Capsid Protein in Viral Replication

探讨登革病毒对人树突状细胞（DC）的感染性。

To study Dengue virus ( DV ) infection of human dendritic cells ( DC ) .

广州市2006年Ⅰ型登革病毒流行株E基因序列分析

Analysis of E Gene of Type I Dengue Virus from the Outbreak in Guangzhou in 2006

收获的抗登革病毒E蛋白第三结构域多克隆抗体具有一定的反应特异性；4。

The obtained polyclonal antibody against domain ⅲ of envelope protein has some specificity ; 4 .

用RT-PCR方法检测登革病毒E基因核酸

Detection of the Envelope Gene of Dengue Virus Type 2 with RT-PCR Method

应用RT-PCR制备登革病毒诊断基因芯片探针

Probe Preparation for Diagnostic Microarray in Detection Dengue Virus by RT-PCR

多引物RT-PCR技术用于登革病毒的检测及快速分型

Multi-primers RT-PCR for the rapid detection and typing of dengue viruses

目的：建立登革病毒的多引物PCR检测方法，并对不同血清型的登革病毒进行快速分型。

Objective : To establish the multi primer PCR assay for detection and typing of dengue viruses .

IL-6与登革病毒感染相互关系的实验研究

Experimental study on interaction between IL-6 and dengue virus infection

RT-PCR扩增Ⅰ型登革病毒核酸及其临床应用

Amplification of Dengue Virus Type 1 RNA by RTPCR and Its Clinical Application

为进一步筛选与登革病毒C蛋白可能存在相互作用的宿主蛋白，本研究分别以表达登革病毒C蛋白的酵母穿梭质粒及人脐带血内皮细胞cDNA文库作为诱饵载体和筛选文库。

Host proteins that interact with dengue virus C protein were screened from human umbilical cord blood endothelial cells cDNA library .

目的：研究TNFα在登革病毒发病中的作用，试图对登革病毒感染发病机理进行探讨。

Objective : To study the effect of TNF α in the pathogenesis of dengue fever .

新近制备了大量纯化的pEH920DNA，该质粒DNA插入了登革病毒2型核酸片段的互补DNA。

Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA .

TNFα与登革病毒感染人脐静脉内皮细胞相互作用的研究

Interaction of TNF α and human umbilical veins endothelial cells from patients with dengue fever in vitro

在登革病毒编码的三种结构蛋白中，包膜E蛋白是病毒的主要结构蛋白，位于成熟病毒颗粒表面，构成病毒颗粒的突起。

In three structural proteins of dengue viruses , the E glycoprotein is the major structure protein on the DENV virion .

人IL-6、TNF-α对登革病毒感染人树突状细胞的影响

Effect of IL-6 and TNF - α on Dengue virus infection of human dendritic cells

方法运用RT-PCR法测定我国3株登革病毒的E基因序列。

Methods The complete genomic sequences of the E gene of three dengue 2 virus strains were determined by RT-PCR .

结果显示，该患者血清中存在抗登革病毒的IgM和IgG抗体。

The result showed that there were anti-dengue virus IgG and IgM antibodies in the serum .

本文应用逆转录聚合酶链反应（RT-PCR）方法，检测Ⅲ型登革病毒基因。

Reverse transcription-polymerase chain reaction ( RT-PCR ) was developed for the detection of dengue virus 3 genome .

中国人4型登革病毒E基因部分片段的cDNA克隆与序列分析

The cloning and sequence analysis of cDNA of the partial E genomic region of Dengue type 4 virus from a Chinese patient

IgM抗体捕捉－生物素亲合素－ELISA检测Ⅳ型登革病毒IgM抗体及其临床意义

Mac Ba ELISA the detection of IgM antibodies to dengue virus type IV and its clinical significance

GM-CSF在登革病毒感染人单核细胞中的作用

Effect of GM CSF on dengue virus infection in human monocytes

结果采用多重PCR，扩增出482、119、290及392bp的特异片段，分别代表登革病毒1～4型。

Results Four specific fragments , of 482,119,290 and 392 bp respectively , were successfully amplified from the 4 dengue virus serotypes by multi-PCR .

抗登革病毒E蛋白单克隆抗体的制备及鉴定登革病毒感染对白纹伊蚊生理生态特性的影响

Preparation and Characterization of Monoclonal Antibody Against DV Envelope Pretein ; The Influence of Dengue Virus Infection Upon the Physiological and Ecological Natures of Aedes albopictus

登革病毒prM-E基因的多价重组质粒DNA的免疫原性研究

Study on Immunogenicity of the Polyvalent Recombinant Plasmids DNA Containing prM-E Genes of Dengue Viruses

两种标签引物RT-PCR结合Sanger测序检测登革病毒的研究

Detection of Dengue Virus Using RT-PCR with Tagged Primers in Combination with Sanger Sequencing

登革病毒是单股正链RNA病毒，基因组全长约11Kb，含有单一开放读码框架。

The genome of dengue virus is a single-strand positive sense RNA of approximately 11 Kb in length , containing a single open reading frame .