疏水层析

中空纤维超滤和疏水层析大规模纯化质粒DNA

Large-scale Purification of Plasmid DNA by Ultrafiltration with Hollow Fiber Filter and Hydrophobic Chromatography

方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒DNA。

Methods Purify plasmid DNA by a procedure consisting of alkaline lysis , ultrafiltration with hollow fiber filter , hydrophobic chromatography and molecular sieve chromatography .

疏水层析用于大规模纯化重组HBsAg的工艺研究

The process study for the large scale purification of recombinant HBsAg by Hydrophobic Interaction Chromatography

结果经过硫酸铵沉淀和Octyl-Sepharose疏水层析纯化，annexinⅤ纯度达到95%。

Results The purity of annexin ⅴ reached up to 95 % after ammonium sulfate precipitation and octyl-sepharose hydrophobic interaction chromatography .

以离子交换、疏水层析和凝胶过滤为基础，建立了rPA的纯化工艺，每升培养物可获得约15mgrPA，纯度可达95%以上。

After a purification procedure including ion exchange , hydrophobic interaction chromatography , and gel filtration , about 15 mg of 95 % pure rPA was obtained from 1 liter culture .

破伤风毒素培养滤液经PhenylSepharose疏水层析除去大部分杂质，再经DEAESephadex离子交换层析进一步纯化。

First most of impurity in the tetanus culture medium can be eliminated with HIC on Phenyl Sepharose , then tetanus toxin can be further purified by the ion-exchange chromatography ( IEX ) on DEAE Sephadex .

方法采用离子交换、分子筛、疏水层析相结合的方法分离纯化兔骨骼肌肌钙蛋白C（sTnC）.制备TnG－Sepharose4B亲和层析柱。

Methods Rabbit skeletal muscles troponin C ( sTnC ) was purified by ion exchange , gel filtration and hydrophobic interaction chromatography to prepare ThC-Sepharose 4B affinity chromatography column .

疏水层析及SephadexG-25Fine凝胶过滤等方法进行分离纯化，比酶活达1096.8U/mg，纯度达88%，纯化倍数达到21.4倍，酶活力回收率为53.9%；

Hydrophobic chromatography , and Sephadex G-25 Fine . The final specific activity of LDH was up to 1096.8 U / mg and the purity was 88 % . 21.4-fold purification was obtained with enzymatic activity recovery of 53.9 % .

疏水层析蛋白质动力学与平衡过程的考察

Adsorption kinetics and equilibrium process of protein in hydrophobic interaction chromatography

方法：采用超滤法截留分离，强阴离子交换层析去除核酸，弱阴/阳离子交换层析和疏水层析组合法精细分离溶栓素。

Methods : Ultrafiltration , ionexchange chromatography , and hydrophobic-interation chromatography were used .

疏水层析介质苯基-琼脂糖凝胶合成与吸附性能的研究

Synthesis and Adsorption Properties of Phenyl - agarose gel

盐析与疏水层析相结合快速分离提纯猪胰激肽释放酶

Rapid Purification of Kallikrein by the Combination of Hydrophobic Interaction Chromatography and Salt Precipitation

方法：对包涵体进行有效洗涤，采用疏水层析进行纯化。

Methods : Wash inclusion body effectivity , use Hydrophobic Interaction Chromatography to carry on the purification .

疏水层析结合冷乙醇沉淀纯化人血清白蛋白

Purification of Human Serum Albumin from Plasma with the Combination of Hydrophobic Interaction Chromatography and Cold Ethanol Precipitation

浙江产眼镜蛇毒胆碱酯酶的研究&Ⅲ、酶的疏水层析行为

Studies on the cholinesterase from the venom of Naja naja atra of Zhejiang Province III. some hydrophobic chromatographic behaviour

用此方法，连续纯化五批毒素，均获得高纯度的破伤风毒素。试验证明破伤风毒素经疏水层析和离子交换层析可得到有效纯化。

We obtained a high purity of tetanus toxin in 5 consecutive batches by using the combined chromatography procedures .

为了获得高纯度的破伤风毒素，用疏水层析和离子交换层析纯化破伤风毒素。

To obtain high purity of tetanus toxin , purification of tetanus toxin was carried out by chromatography procedures .

多孔聚（甲基丙烯酸环氧丙酯-二乙烯基苯）微球的改性及作为蛋白质疏水层析介质的应用

Modification of Poly ( Glycidyl Methacrylate-Divinylbenzene ) Porous Microspheres with Polyethylene Glycol and Their Use as HIC Medium for Protein Adsorption

以交联琼脂糖为基质，采用直接偶联法和活化偶联法制备了苯基琼脂糖疏水层析介质。

Two different methods , direct coupling and coupling after activation , have been used to prepare the hydrophobic interaction chromatography media .

以琼脂糖凝胶为基质，与2,3-环氧丙烷苯基醚反应后，制得疏水层析介质苯基-琼脂糖凝胶。

The phenyl-agarose gel was prepared by reacting 2,3-glycidol phenyl ether with Sepharose CL-4B and its physical and chemical properties were determined .

以疏水层析的方法将海因酶、N-氨甲酰水解酶进行初步分离，并调节双酶比例进行了转化试验。

D-HEn and N-CAEn were separated using HIC , and the ratio of the two enzymes was adjusted and the experiment results show that the conversion process is promoted sharply .

目的蛋白经硫酸铵沉淀、疏水层析、分子筛层析和阴离子层析得到纯度超过90%的样品。

Coli . After Ammonium sulfate precipitation , hydrophobic interaction chromatography , Gel filtration chromatography and anion exchanger chromatography , protein sample that has the purity above 90 % was achieved .

着重介绍包涵体纯化技术，包括金属亲和层析，凝胶过滤层析，离子交换层析，疏水层析，双水相萃取技术和反胶团相转移技术近年来的进展情况。

In this article , we review the progress of inclusion body purification techniques , including metal affinity chromatography , gel-filtration chromatography , ion exchanger chromatography , hydrophobic interaction chromatography , aqueous two phase and the phase transfer method of reverse micelles .

疏水作用层析在重组大肠杆菌表达的rProEMAPⅡ/P43蛋白纯化中的作用

Role of Hydrophobic Interaction Chromatography in Purification of rProEMAP ⅱ / P43 Protein Expressed in E.coli

而将疏水作用层析与离子交换层析相结合，即可开发出具有混合作用模式（mixed-mode）的耐盐性吸附剂，就可以使吸附作用不再依赖于盐浓度。

And that combine the ion exchange chromatography ( EEC ) with the hydrophobic interaction chromatography ( HIC ), a mixed-mode and salt-tolerant adsorbent was exploited and the protein binding capacity of the prepared adsorbent is independent on the salt concentration of the solution .

平菇萃取液经酸性沉淀、热变性、Phenyl-SepharoseCL-4B疏水亲和层析和DEAE-Cellulose52离子交换层析分冉出了电泳纯的真菌钙调素。

Mushroom calmodulin ( CAM ) proved to be electrophoretic homogeneity is obtained in extract of pleurotus ostreatus hymenophore by means of acidic isoelectric point precipitation , heating treatment , Phenyl-Sepharose CL-4B hydrophobic chromatography and DEAE-Cellulose 52 ion exchange chromatography .

疏水作用层析及其渗透质辅助包涵体蛋白质复性的研究

Study on Inclusion Body Protein Refolding Assisted by Hydrophobic Interaction Chromatography and Osmolytes

蛋白质层析用离子交换和疏水作用层析介质的发展概况

Advancement of Ion Exchange Chromatography and Hydrophobic Interaction Chromatography Media Application in Protein Chromatography

疏水柱层析还用于监测在低浓度胍的作用下蛋白质的构象变化。

The conformational change of proteins in the low concentration of guanidine was also followed .

本论文的目的在于利用疏水作用层析技术开发一种新的疏水作用层析辅助包涵体蛋白质复性方法，实现蛋白质在高浓度下的高活性和高回收率。

The objective of this research is to develop a hydrophobic interaction chromatography assisting protein renaturation method in order to meet the challenge .