甲型肝炎病毒

一步单管反转录PCR快速检测水中甲型肝炎病毒

Rapid Detection of HAV in Water by the One-Step Single-Tube RT-PCR

多重PCR检测水中肠道总病毒及甲型肝炎病毒

Detection of Enteroviruses and Hepatitis A Virus in Water by Multiplex PCR Method

甲型肝炎病毒PCR检测结果与其感染性关系的研究

Study on the relationship between result of PCR of HAV and its infectivity

应用逆转录PCR检测海产贝壳类中的甲型肝炎病毒

Detection of HAV in Shellfish by RT-PCR

甲型肝炎病毒（H2株）在三种细胞中的感染性滴度比较

The Comparison of Infectious Titers of Hepatitis A Virus ( H 2 Strain ) in Three Kinds of Cells

甲型肝炎病毒编码区cDNA在减毒伤寒杆菌中的高效表达

The expression of hepatitis A virus coding region cDNA in attenuated Salmonella typhimurium

用甲型肝炎病毒单克隆抗体包被，ELISA夹心法检测表达产物，效价为1∶16。

Hepatitis A virus antigen expressed was 1 ∶ 16 in titer by sandwich ELISA .

用ELISA捕获法测抗甲型肝炎病毒IgG及与竞争法测抗甲型肝炎病毒的比较

Detection with Capture ELISA of Anti-HAV IgG and Comparision with Contest ELISA of Anti-HAV

用多聚酶链反应检测环境样品中甲型肝炎病毒RNA的实验研究

Detection of Hepatitis A Virus in Environmental Samples by Coupled Reverse Transcription and Polymerase Chain Reaction

应用化学发光自显影技术检测甲型肝炎病毒和人IgM

Application of Chemiluminescent Photographic Detection in Identification of HAV and Human IgM

目的：建立斑点免疫渗滤试验检测甲型肝炎病毒IgM抗体的实验方法。

AIM : To establish a dot immunofiltration method for the detection of anti HAV IgM .

固相免疫吸附血凝抑制试验快速检测甲型肝炎病毒IgM

Rapid detection of hepatitis A virus antibody IgM by solid - phase immunosorbent hemagglutination inhibition test

根癌农杆菌介导甲型肝炎病毒（HAV）衣壳蛋白融合基因转化柑桔的研究

Agrobacterium-mediated Transformation of Citrus with Hepatitis A Virus Capsid Protein Fusion Gene

恒河猴猴群中甲型肝炎病毒（HAV）血清学调查

Investigation of Serum Antibody to Hepatitis A Virus in the Rhesus Monkeys

人源中和性抗甲型肝炎病毒抗体Fab段基因的序列分析及可溶性表达

Sequence Analysis and Gene Expression of Neutralizing Recombinant Human Fab Antibody Fragments to Hepatitis A Virus

不同甲型肝炎病毒株间部分编码VP1N端的核苷酸序列比较

Comparison of partial nucleotide sequence coding for vp_1 N-terminus among hepatitis A virus strains

目的获得人源性甲型肝炎病毒（HAV）抗体。

Objective To obtain the anti-HAV antibodies .

本文综述了甲型肝炎病毒的病原学、食品中甲肝病毒检测情况、ELISA方法和基因芯片检测甲肝病毒研究进展。

The research progression of etiology of hepatitis A , detected condition of hepatitis A from food , and diagnosis of hepatitis A by ELISA and gene chip were summarized .

Pichiapastoris酵母中表达人源中和性抗甲型肝炎病毒scFv-Fc融合抗体的研究

Expression of Human Neutralizing Recombinant scFv-Fc Fusion Antibody to Hepatitis A Virus in Pichia pastoris

甲型肝炎病毒感染2BS细胞后培养液中甲肝病毒的研究

Study on HAV in the cultural medium of the infected 2BS cells

连续传20代后提取病毒DNA做Southernblot杂交表明，甲型肝炎病毒基因仍稳定地存在于原插入位置。

Southern blot hybridization showed that the inserted HAV cDNA was still located at the correct site after the recombinant virus had undergone 20 passages in the diploid cell culture , indicating the stability of the HAV gene in the vector .

应用cDNA-RNA分子杂交法检测临床粪便标本中的甲型肝炎病毒

Detection of hepatitis A virus from clinical specimens by cdna-rna hybridization

甲型肝炎病毒（HAV）分布于全世界，是一种主要的急性传染性肝炎。

Hepatitis A virus ( HAV ), a major cause of acute hepatitis infections in humans in many countries , is a positive strand RNA virus belonging to the picornavirus family .

但由于电镜法价格昂贵，对环境及操作人员要求较高；ELISA的灵敏度不及RT-PCR，使得RT-PCR成为目前检测轮状病毒、诺如病毒和甲型肝炎病毒的金标准。

However , electron microscopy is very expensive and has required the higher environment and operators . The sensitivity of ELISA is less than RT-PCR . Therefore , RT-PCR has become the " gold standard " of RV , NV and HAV detection .

用甲型肝炎病毒（HAV）HM175株感染恒河猴胚肾细胞系（Frhk－4），经过连续传代培养，建立了一株持续感染HAV的细胞株。

A persistent infection of hepatitis A virus ( HAV ) strain HM-175 was established in a fetal rhesus monkey kidney cell line ( Frhk-4 ) .

阴离子交换色谱纯化甲型肝炎病毒（YN5株）的研究

Studies on the purification hepatitis A vaccine ( YN5 strain ) with anion exchange chromatography

为了探讨氯灭活甲型肝炎病毒（HAV）的机制，采用酶联免疫吸附试验和RNA的随机引物扩增指纹图谱技术分别检测在氯灭活HAV前后病毒抗原以及核酸多态性的变化。

The mechanisms of HAV inactivated by chlorine was investigated by enzyme-linked immunosorbent assay and RNA fingerprinting . Arbitrary primed PCR were involved to detect the changes of antigenicity and RNA polymorphism respectively before and after inactivation by chlorine .

表达甲型肝炎病毒抗原的重组痘苗病毒（VMS11HAV25）的免疫原性

Immunogenicity of a recombinant vaccinia virus ( vms11 hav 25 ) expressing hepatits a virus antigens

用抗甲型肝炎病毒（HAV）IgG阴性血清处理以抑制非特异反应，提高灵敏性的捕获法酶联免疫吸附试验检测甲型肝炎病毒特异IgG抗体，并与竞争法检测总抗HAV进行了比较。

A r-capture ELISA for detecting IgG antibody to Hepatitis A virus ( HAV ) has been developed and compared with contest ELISA of anti-HAV . It is useful that anti-HAV IgG negative sera inhibit nonspecific reaction in the capture ELISA of anti-HAV IgG .

用免疫荧光（IF）试验证明，约有50%细胞的胞浆内有甲型肝炎病毒抗原（HAAg）。

It was named strain BJ-1 . It was demonstrated by immunofluorescent method that hepatitis A virus antigen ( HAAg ) existed inside the cytoplasm of about 50 % of A549 cells .