半固体培养基

MTT-半固体培养基测定细菌动力的研究

MTT Semi-solid medium for the detection of bacterial motility

结果：在富含有机氮的半固体培养基上蜜环菌最易形成粗壮的菌索。

Result : The strong shoestrings were easily restored in the semi-solid medium with rich organic nitrogen source .

MSC在含有造血因子的半固体培养基中培养4～5周未见造血细胞集落形成。

After 4 to 5 weeks'culture , no hematopoietic cell colony formation was observed from the MSC .

诱导后的组3细胞呈低增殖状态（PI值约为10.4%），在半固体培养基中还可形成血管腔样结构。

The induced cells of group 3 showed low proliferating state ( PI was 10.4 % ) and formed capillary-like structure in semisolid medium .

在半固体培养基中的AngⅡ则仅刺激cFUGM的形成，却不影响BFUE；

In semi solid culture assay , Ang ⅱ stimulated CFU GM production but no effect on BFU E occured .

取第3代MSC在含有造血因子的半固体培养基中培养。

MSC were culture in semisolid culture containing hemopoietic growth factor for 4 to 5 weeks to observe the hematopoietic cell colony formation .

通过分析细胞的生长曲线、分裂指数及在半固体培养基中的集落形成率比较细胞的生长特性，采用RT-PCR检测c-myc及β-珠蛋白（β-globin）基因的表达。

The changes of cell growth rate , mitotic index and colony - forming rate in semi - solid medium were investigated . The expressions of c - myc and β - globin genes were analysed by semi - quantitative RT - PCR .

半固体培养基快速测定法在不同食品中的应用研究

Rapid Detection of Coliform in Foods with Semi-solid Culture

目的制成一种容易测定细菌动力的半固体培养基。

Objective To develop a semi solid medium for the easy detection of bacterial motility .

采用半固体培养基有效缩短了培养周期，减少了细胞污染的概率。

By comparison of liquid culture medium , the semi-solid medium was adopted and the clone cycle was shortened with decreased polluting rate of the hybridomas . 3 .

利用双层半固体培养基具有良好厌氧性的特性，在实验的基础上，提出了采用方形试管对双歧杆菌进行计数的方法，介绍了该方法的基本原理和操作步骤。

Based on the experiment , the enumeration method of Bifidobacterium by rectangular test tube was put forward , according to the anaerobic specificity of double layer half solid culture .

文中通过检测乳酸菌在不同固体和半固体培养基中的菌落数、菌落大小及溶钙圈大小，以筛选出乳酸菌生长的最佳培养基。

The optimal medium for the growth of lactics was chosen by detecting the number , size of colony and the size of calcium dissolving zone when the bacteria grew in different solid and semisolid medium .

采用液体培养基管、半固体琼脂培养基管、无菌干空针与无菌干空管等方法同时送检厌氧菌培养标本；

Three transport systems are introduced , the liquid and semisolid agar medium tubes and the sterilized dry aerobic syringe , comparing with that of the sterilized dry aerobic tube for culture of anaerobic bacteria .