半固体

诱导后的组3细胞呈低增殖状态（PI值约为10.4%），在半固体培养基中还可形成血管腔样结构。

The induced cells of group 3 showed low proliferating state ( PI was 10.4 % ) and formed capillary-like structure in semisolid medium .

取第3代MSC在含有造血因子的半固体培养基中培养。

MSC were culture in semisolid culture containing hemopoietic growth factor for 4 to 5 weeks to observe the hematopoietic cell colony formation .

用半固体的液体培养，观察前列腺素E2对人外周血T淋巴祖细胞增殖和T淋巴细胞转化的调节。

Prostaglandin E2 ( PGE2 ) modulation of human T cell colony forming unit and lymphocyte transformation in vitro is reported .

半固体琼脂法进行骨髓CFU－GM测定。

CFU-GM was measured using the semi-solid agar method .

方法骨髓细胞半固体培养和悬浮培养，DNA凝胶电泳，DNA缺口末端标记（TdT），电镜观察。

Methods Semisolid culture , DNA agarose electrophoresis , in situ terminal deoxynucleotidyl transferase assays , view with electromicroscope .

MSC在含有造血因子的半固体培养基中培养4～5周未见造血细胞集落形成。

After 4 to 5 weeks'culture , no hematopoietic cell colony formation was observed from the MSC .

半固体集落培养法观察激活骨髓cFUGM水平和对白血病细胞的净化作用；

The numbers of CFU GM and action of purging of ABM were counted by semi solid culture of colonies .

为了了解重组白细胞介素2对骨髓造血祖细胞体外增殖的影响，应用体外半固体培养法研究了rhIL－2及其与GM－CSF和Epo组合对骨髓祖细胞集落形成的调节作用。

In order to determine the regulation effects of rhIL 2 on hematopoietic progenitor cells from bone marrow .

在半固体培养基中的AngⅡ则仅刺激cFUGM的形成，却不影响BFUE；

In semi solid culture assay , Ang ⅱ stimulated CFU GM production but no effect on BFU E occured .

方法分别用SLE患者及正常人B、T及B+T淋巴细胞加入半固体甲基纤维素培养体系中，观察它们分别对16例SLE患者及12名正常人GMCFU产率的影响。

Methods GM CFU of 16 active SLE patients and 12 controls was cultured and observed in vitro in semisolid methylcellulose with B lymphocytes and T lymphocytes respectively and together .

通过解剖镜下计数脾结节、半固体琼脂法检测CFU-GM及常规计数外周WBC变化进行造血功能测定。

The hematopoietic function was evaluated with the spleen node count under microscope , semi-solid agar CFU-GM assay and peripheral blood WBC count .

应用半固体琼脂培养法和MTT法研究了极大螺旋藻胞内多糖对人血癌细胞U937和HL－60的影响。

The effects of polysaccharides from Spirulina maxima on the growth of human leukemia cells were studied by semi-solid agar culture and MTT staining .

方法：用液体或半固体培养法及流式细胞术测定血小板因子4对CD34阳性脐血细胞的增殖与分化的作用。

Methods : Liquid and semi solid culture systems as well as flow cytometry were used to study the effect of PF4 on the proliferation and differentiation of CD34 + cord blood cells .

方法：采用末梢血非粘附单个核细胞进行体外无血清液体培养及半固体培养，应用RTPCR技术检测液体培养自发性生长细胞的cMpl及GPⅡb的表达。

Methods : Non adherent mononuclear cells ( NMNC ) from peripheral blood of the patients were cultured in serum free medium and the expressions of c mpl and GP ⅱ b were examined by means of RT PCR technique in the spontaneous growth cells .

PNH患者CD34+CD59+细胞及CD34+CD59-细胞体外半固体培养，其形成CFU的能力无明显差异。

In terms of semisolid culture , there was no significant difference in the yields of CFU formation between CD 34 + CD 59 + and CD 34 + CD 59 - cells .

通过分析细胞的生长曲线、分裂指数及在半固体培养基中的集落形成率比较细胞的生长特性，采用RT-PCR检测c-myc及β-珠蛋白（β-globin）基因的表达。

The changes of cell growth rate , mitotic index and colony - forming rate in semi - solid medium were investigated . The expressions of c - myc and β - globin genes were analysed by semi - quantitative RT - PCR .

取E10.5小鼠胚胎AGM区，消化成单细胞，在无血清培养体系下孵育12h后进行半固体集落培养。

Obtain E10.5 mouse AGM region , and digest it into single cells , after 12h incubation in serum-free incubation culture system , then culture colonies in the semi-solid medium .

本研究以半固体发酵方式培养泡盛曲霉（Aspergillusawamori）生产植酸酶，通过超滤、阴离子交换层析，凝胶层析等步骤，纯化了植酸酶蛋白，分子量约为118kDa。

Extracellular phytase produced by Aspergillus awamori using technique of semi-solid cultivation was purified to homogeneity by employing an initial ultrafiltration step , followed by chromatography using anion exchange , gel filtration and chromatofocusing steps .

材料与方法：用甲基纤维素半固体培养法对14份新鲜脐带血和26份新鲜胚胎血标本的单个核细胞（MononuclearCells，MNC）进行CFU-GM体外培养。

Material and Methods : Granulocyte / macrophage progenitor assays ( colony forming unit - granulocyte / macrophage , CFU-GM ) were established with mononuclear cells ( MNC ) from 14 fresh cord blood samples and 26 fresh fetal blood samples by using the methylcellulose semisolid culture method .

方法：在液体培养、半固体集落形成培养中，以不同浓度的GPT处理K562细胞24、48、72小时，用细胞生长曲线和集落形成率来观察GPT的增殖和抑制效应；

Methods : K562 cells were dealed with different concentrations for 24h , 48,72 hours in the culture of liquid , semi solid colony . And the effect of GPT 's proliferation and inhibition were observed by cell growth curve and colony forming rate .

同时采用甲基纤维素半固体培养法进行巨核细胞集落培养，测定巨核细胞集落形成单位（CFU-MK）的数量。

After 15 days of culture , the content of CD41 + cells in culture system were determined by flow cytometry , and the number of megakaryocyte colony-forming unit ( CFU-MK ) was measured simultaneously .

采用甲基纤维素半固体培养法，观察了再生障碍性贫血（再障）患者CD8~+细胞对其自身T淋巴细胞集落形成单位（CFU-TL）的影响。

The effects of CD8 + celis from patients with aplastic anemia ( AA ) on growth of self T lympho-cyte colony - forming unit ( CFU - TL ) in vitro were observed .

采用放射性核素99Tcm植酸钠标记的半固体试餐，单光子放射计算机断层显象术（SPECT）检测胃半排空时间（GEt12）；

Gastric half emptying time ( GE t1 / 2 ) was monitored and analysed using single photon emission computerized tomography ( SPECT ), and the half solid test meal was labeled with an isotope 99 Tc m sulfur colloid .

本文运用半固体琼脂培养法及MTT法研究了红藻藻蓝蛋白（R-PC）对HL-60细胞生长的影响，结果发现：R-PC能够显著抑制细胞的生长，且存在浓度效应和时间效应关系。

The effect of phycocyanin from Porphyra haitanensis on the growth of human leukemia HL-60 cells was studied by semi-solid culture method and MTT staining assay . The results showed that the phycocyanin could significantly inhibit the growth of HL-60 cells in a dose - an time-dependent manner .

方法：将小鼠骨髓单个核细胞（BMNC）按（2,4,6,8,10）×105个/mL浓度加入半固体培养体系，体外培养巨核细胞集落形成单位（CFU-Meg），观察BMNC数与CFU-Meg生成数之间的关系；

Methods ( 2,4,6,8,10 )× 10 ~ ( 5 ) / mL bone marrow single nucleus cells ( BMNC ) were added in the culture system of colony forming unit-megkaryocyte ( CFU-Meg ) to find out the relationship of the cultured BMNC with the output of CFU-Meg .

安瓿半固体保存菌种时间观察

Observation on the duration of semisolid preservation of bacterium in ampule

血液病病人骨髓粒系造血祖细胞体外琼脂半固体培养

In vitro semi-solid culture of CFU-GM from patients with blood diseases

要测量半固体和固体沥青混合料的相合性。

To measure the consistancy of semi-solid and solid bituminous mixtures .

在常温下，渣油为黑色粘稠半固体状态的物质。

The residual oil is the black sticky semi-solid state material .

10-羟基喜树碱半固体脂质纳米粒的研制与稳定性考察

Preparation of 10-hydroxycamptothecin Semisolid Lipid Nanoparticles and Investigation of Its Stability