蔗糖培养基

从小麦根际土中分离得到一种能取食B1和B2两种细菌的土壤食细菌线虫，并在马铃薯蔗糖培养基上富化培养成功。

A soil bacterivorous nematode feeding on bacteria B1 and B2 was isolated from rhizosphere soil of wheat and cultured on potato sucrose agar medium ( PSA ) .

方法：将发酵乳杆菌加入含不同浓度CPP-ACP的蔗糖培养基中，厌氧培养48h，用精密pH计检测培养基上清液的pH值，计算pH的变化值ΔpH（初始pH值-终末pH值）。

METHODS : Lactobacillus fermentum was inoculated in medium containing 1 % sucrose and different concentrations of CPP-ACP and grew in anaerobic condition at 37 ℃ for 48 hours . The primary pH values and final pH values of the supernate were measured , then the △ pH was compared .

将不同发育时期的小麦花药在C（17）、高K~+高蔗糖浓度培养基上培养。

Wheat anthers in different development stages were inoculated respectively under three different cultural medium : C17 , high K ~ + concentration and high sugar concentration medium .

生物学特性研究表明，适于番茄黄萎病菌生长的培养基为PSA培养基，低量蔗糖酵母培养基，马铃薯胡萝卜培养基及蔡氏培养基；

Biological characteristic research indicated : the culture mediums that were suitable for tomato Verticillium .

用自来水、食用白糖配制培养基，生根采取液体培养15d，比用蒸馏水、蔗糖配制培养基，生根采取固体培养30d，试管苗的直接生产成本可降低28%。

So using tap water , white sugar and reducing rooting culture time to 15 days in liquid medium instead of using distilled water , sucrose , rooting culture 30 days in solid medium can reduce 28 % of the cost of tube seedling .

在10%蔗糖发酵培养基中，发酵液中葡萄糖和果糖的积累量和积累方式发生改变。

On the other hand , amount and fashion of accumulation of glucose and fructose alter in fermentation culture medium containing 10 % sucrose .

水和滤纸做成纸桥为发芽床种子发芽率达68.3%；1/2MS+BA1.0+NAA0.1+3%蔗糖的培养基上种子发芽率仅为45.0%。

The germination rate in the filter paper bed with water was 68.3 % , and 45.0 % in the 1 / 2 MS medium with BA1.0 + NAA0.1 + 3 % sugar .

用凉开水替代蒸馏水，白砂糖代替蔗糖配制培养基，可显著降低了组培苗生产成本，提高了灰毡毛忍冬新品种工厂化育苗的生产效益。

Production cost can be significantly reduced when using plain boiled water and white granulated sugar instead of distilled water and sucrose , which increased productivity effect of factory nursery of Lonicera macranthoides new varieties .

低浓度的蔗糖琼脂培养基对花粉发育有利，高浓度的培养基对梅的花粉发芽有抑制作用。②梅是自花能结实的果树，但异花授粉更能增产。

A low concentration to pollen Suerose agar medium is favourable to germination while high medium concentration may Control pollen Sprouting , and ② mume fruits-when it is Self pollinated , but ite yield increases when it is cross plllinated .

采用MS+BA2+NAA（0.3）+2%蔗糖分化培养基研究证明，20年生植林茎尖的分化频率平均为10%。

For shoot differentiation a medium with MS + BA_2 + NAA_ ( 0 · 3 ) + 2 % sucrose was used and an average frequency of 10 % was obtained is shoot tip culture collected from the tree of 20 years of age .

BA、NAA和2，4－D对沙打旺叶片愈伤组织分化成苗有调节作用。此外，还探讨了蔗糖和不同培养基对愈伤组织形成的影响。

BA , NAA and 2,4-D had controlled the effect on the leaf of Astragalus adsurgens , and also effects of cane sugar and different media on callus formation .

丛生芽的生长中以30%蔗糖浓度的培养基为佳；

30 % sucrose media is the best for fascicular bud grow .

同时，通过对无菌苗生理指标的测定，添加1%蔗糖的1/2MS培养基有利于壮苗的形成。

And the sterile plants in the1 / 2 MS medium with 1 % sucrose are more healthy by comparing their physiological index .

幼胚愈伤组织在含5%蔗糖的基本培养基附加10%椰乳的培养基上，白色胚状体发生率最高。

Embryogenic calli yielded most white embryoids when inoculated on MT medium added 5 % sucrose and 10 % CW ( coconut milk water ) .

整花散粉方式以0.75%琼脂+20%蔗糖+0.02%硼酸培养基的培养效果最好，而花药散粉以0.75%琼脂+10%蔗糖+0.01%硼酸培养基的培养效果最好，其花粉萌发率分别为82.75%和31.43%。

The culture medium that contained 0.75 % agar , 10 % sucrose and 0.001 % boric acid was best for the germination of pollen from anther , and the pollen germination rate was 82.75 % and 31.43 % , respectively .

方法：考察了蔗糖质量浓度、培养基pH、接种、植物生长物质等影响因子对丹参不定根的生长及其次生代谢产物含量的影响。

Method : Effects of sucrose concentrations , medium pH , inoculum size and plant growth regulators on adventitious root growth and secondary metabolites production in S. miltiorrhiza were investigated .

小孢子单核期为适宜的诱导时期，培养基以6%～13%蔗糖浓度的B5培养基较好；

The uninucleate microspore stages and B5 medium with sucrose from 6 % to 13 % were optimal for callus induction .

结果表明：悬铃木花粉在15%蔗糖+0.01%硼酸的培养基上培养24h后萌发率最高；

The result showed that the pollen germinating percentage reached the highest after incubated for ( 24 h ) on the media supplemented with 15 % sucrose and 0.01 % Boric acid ;

取材以2mm～3mm的花蕾效果最好，培养基则是含6%～10%蔗糖浓度的B5培养基最好。

Flower buds 2mm ~ 3mm in length were most desirable , and B5 supplemented with 6 % ~ 10 % sucrose appeared to be the optimum medium .

结果表明：WPM+3%蔗糖为最佳基本培养基。

Results showed that the best basic medium was the WPM medium supplemented with 3 % sucrose .

诱导不定根，在1/2MS+1.5%蔗糖+0.5%琼脂+0.10mg/LNAA培养基上生根率为100%，达到植株再生和快繁的目的。

The ideal culture medium for rooting is 2 / 1MS + 1.5 % cane sugar + 0.5 % agar + 0.10 mg / LNAA of with 100 % rooting rate which is reach to plant regeneration and micropropagation .

在10%蔗糖+0.5%硼酸和15%蔗糖+0.5%硼酸培养基上花粉发芽率均为0。

In the culture medium of " 10 % sugar + 0.5 % boric acid " and " 15 % sugar + 0.5 % boric acid ", pollen germination percentages of all the cultivars were 0 .

研究糖和无机氮源对白榆组培新梢不定根形成的影响，结果表明，含有高浓度蔗糖的培养基比低浓度蔗糖的培养基新梢生根率高。

The result of study on effect of sugar and inorganic nitrogen to rooting of elm tip in tissue culture showed that rooting rate of new tip was higher in higher sugar level medium than in lower sugar level medium .

用春性甘蓝型油菜为材料进行蔗糖浓度的实验结果表明：培养3天后，在16%蔗糖培养基中存活的小孢子最多，达16.13%；

The results of experiments on sucrose concentration with the spring Brassica napus showed that the number of microspores , survived after 3 days culture in the medium added with 16 % sucrose were the largest , as high as 16.13 % .

蔗糖对红景天甙含量的合成有影响，加3.5%蔗糖的MS培养基中生长的愈伤组织有利于红景天甙的合成。

4 , Sucrose had significant effects on the accumulation of salidroside and MS medium containing 3.5 % sucrose was the best .