牛胰岛素

牛胰岛素A链的合成及其与天然B链组合成结晶胰岛素

Synthesis of A-chain of bovine insulin and partial synthesis of crystalline bovine insulin from synthetic a - and natural B-chains

本文报导了牛胰岛素A链的S-磺酸盐（Ⅰ）的合成以及自Ⅰ和天然B链S-磺酸盐（Ⅱ）组合为结晶牛胰岛素的半合成。

Crystalline bovine insulin has been synthesized from the S-sulphonate derivatives of synthetic A-and natural B-chains of bovine insulin , and the A-chain derivative (ⅰ) has been prepared from synthetic peptide intermediates .

其中5种可与牛胰岛素原C肽结合，但与牛胰岛素未见反应，提示其抗原决定簇均位于前者的部分；

Five of the monoclonal antibodies could bind with C-peptide of bovine proinsulin but not with bovine insulin , suggesting that their antigenic determinants was in the part of C-peptide of bovine proinsulin .

试剂和药品：DMEM培养基，牛胰岛素，庆大霉素，胎牛血清，DPBS均为GIBCO公司产品；

Reagents and drugs : DMEM medium , bovine insulin , gentamicin , fetal bovine serum ( FBS ) and DPBS were purchased from GIBCO Ltd.

毛细管区带电泳监测牛胰岛素的去折叠过程

Monitoring the Process of Bovine Insulin Unfolding by Capillary Zone Electrophoresis

用抗牛胰岛素单克隆抗体鉴别不同种属胰岛素

Use of Monoclonal Antibodies against Bovine Insulin to Distinguish Different Species of Insulin

口服牛胰岛素在昆明株小鼠抑制了链脲菌素引起的糖尿病及胰岛炎

Suppression of insulitis and diabetes in Kunming mice by oral bovine protamine zinc insulin

纯化蛋白的氧化还原活性通过二硫苏糖醇还原牛胰岛素的反应得到证实。

The redox activity of the purified protein was confirmed by reduction of insulin disulfides .

含半胱氨酸多肽的研究&Ⅲ.牛胰岛素A链带保护基的氨端九肽的合成

Cysteine peptides & iii. synthesis of a protected N-terminal nonapeptide of the A-chain of bovine insulin

肽的研究&Ⅴ.新的带保护基牛胰岛素A链氨端五肽和九肽的合成

Studies on peptides & ⅴ . the synthesis of new protected N-terminal pentapeptide and nonapeptide of the A-chain of bovine insulin

C~（14）-标记牛胰岛素A链和C~（14）-标记牛胰岛素的合成

Synthesis of c ~ ( 14 ) - labeled A-chain of bovine insulin and c ~ ( 14 ) - labeled bovine insulin

这个结果表明，重组HbTRX能降低牛胰岛素二硫化物的结合，具有氧化还原活性。

This shows that , the recombinant protein in E. coli , can reduce bovine insulin disulfides bridges , with weak redox activity .

利用弱阴离子交换色谱法在最优化条件下可使还原变性牛胰岛素的二硫键对接率由2.6%提高到33.5%。

The protein refolding efficiency of reduced / denatured insulin can be increased from 2.6 % to 33.5 % by using weak ion-exchange chromatography .

所设计制备的葡萄糖、过氧化氢、胆碱、牛胰岛素等生物传感器可在低电位下具有较高的灵敏度、较低的检测下限以及快的响应速度，并能很好的应用于实际样品的检测。

The developed glucose , hydrogen peroxide , choline and bovine insulin biosensors have high sensitivity , low detection limit and fast response time in low potential .

人工牛胰岛素的合成，是生命科学领域的重大突破，对于人类探索生命奥秘的影响是巨大的。

Synthetic bovine insulin marked a significant breakthrough in the fIeld of life science and has substantially influenced human endeavors in finding out the secrets of life .

另外，使用体积排阻色谱，疏水色谱，离子交换色谱法对还原变性的牛胰岛素的再折叠特性进行了研究。

Secondly the refolding of reduced-denatured insulin from bovine pancreas with size-exclusion chromatography ( SEC ), HIC , and ion-exchange chromatography ( IEC ) was also investigated , respectively .

用绝对分析法定量测定了牛胰岛素在不同水解条件下水解液中氨基酸的含量，大部分氨基酸的相对收率都接近100%。

The amino acids ′ contents of bovine insulin hydrolysate in different conditions are measured quantitatively by absolute analysis method , and the relative recovery of the amino acids is nearly 100 % .

在1980年重组DNA技术出现使得药厂制造人类胰岛素出现以前，猪及牛的胰岛素已经成为为糖尿病患者治疗常规使用的药物。

Before recombinant DNA technology enabled pharmaceutical companies to manufacture human insulin in the1980s , pig and cow insulin were routinely given to diabetic patients .

牛类胰岛素生长因子结合蛋白3基因PCR-SSCP分析

PCR-SSCP Analysis on the Insulin-like Growth Factor Binding Protein 3 Gene in Beef

牛类胰岛素生长因子IGF-I基因研究

Sequence Variations on the Bovine Insulin-like Growth Factor-I Gene

研究了牛初乳类胰岛素生长因子在不同温度、pH值、添加保护剂条件下对其降糖活性影响，并利用Balb/c3T3成纤维细胞糖代谢测定了IGFs降糖活性。

The paper studied the activity of bovine colostrums insulin-like growth factors to reduce glucose concentration at different temperature and pH and by adding protective reagent . We tested the metabolic effect of bovine colostrums IGFs on balb / c3T3 fibroblast .

肥胖牛易发生胰岛素抵抗，使得胰岛素的敏感性下降，影响胰岛素对体内糖、脂肪的代谢的调节。

The insulin-resistance may occur in fat cow which decreases sensitivity to insulin , and influences adjustment of insulin on glucose and fat metabolism .

小剂量牛初乳短链胰岛素样生长因子-Ⅰ可改善糖尿病大鼠周围神经病变

Bovine colostrum truncated insulin like growth factor - ⅰ ameliorates peripheral neuropathy in diabetic rats

在梯度淋洗条件下，卵清蛋白、牛血清蛋白、胰岛素、γ-球蛋白、核糖核酸酶、溶菌酶获得了较好的分离。

With a column of 50 mm long under gradient elution conditions , a mixture of proteins consisting of albumin , bovine serum protein , insulin , γ _ globulins , ribonuclease and lysozyme can be separated successfully in 15 min.

第一个人工合成牛胰岛�1965年，我国的科学工作者经过6年多坚持不懈的努力，获得了人工合成的牛胰岛素结晶。

Synthetic bovine insulin , which was fully developed in 1965 following 6 years of hard work by a group of Chinese scientists , was the first case of human beings synthesizing live proteins .