片段分析
- 网络Fragment Analysis
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背景:由于测序和片段分析中的先进技术,DNA测序仪和分析仪在短时间内就产生了大量的数据。
BACKGROUND : Due to the advanced techniques in sequencing and fragment analysis , DNA sequencers and analyzers produce vast amounts of data within short time .
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基于毛细管电泳片段分析的MJD/SCA3基因诊断
Gene diagnosis of MJD / SCA3 based on fragment analysis with capillary electrophoresis
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山羊与岩羊间特异RAPD片段分析
Analysis of specific RAPD fragments between goats and Pseudois nayaurs
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中国栗属特有种保守RAPD片段分析
Analysis on Conservative DNA Segment for Different Species of Castanea in China by RAPD Marker
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白菜核雄性不育两用系花蕾的mRNA差别显示及其cDNA差异片段分析
Differential display of flower bud mRNA of genic male sterility ( GMS ) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragments
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样品的PCR产物经直接测序及限制性酶切片段分析,以进一步鉴定扩增结果。
The amplified specific sequence of the bovine mtDNA from meat and feed samples is demonstrated by both direct sequencing and restriction endonuclease digestion yield analysis .
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卵母细胞介导的乙肝病毒基因在早期鼠胚中的复制与表达1例早期HIV感染者体内病毒准种的各基因片段分析
Replication and Expression of Oocyte-Mediated HBV DNA in Early Mouse Embryo ; Analysis of HIV quasispecies according to different gene regions during primary HIV-1 infection
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该方法用异硫氰酸胍提取进口饲料样品DNA,以牛特异性引物扩增模板DNA,扩增产物为271bp,PCR产物经限制性酶切片段分析,与预期设想一致;
DNAs were isolated from import feedstuff samples by guanidine isothiocyanate method . Bovine specific primers were synthesized and applied in the amplification and the length of target product was 271 bp .
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冷血和温血动物血吸虫28SRRNA基因的扩增和酶切片段分析
The amplification and endonuclease analysis of 28s rRNA gene of blood flukes
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对得到的该片段分析表明,它是一种脂肪转移蛋白(LTP)基因的部分序列。
Sequence analyses revealed that it is a partial sequence of a lipid transfer protein gene .
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方法:应用毛细管电泳片段分析(STR分析)对MJD/SCA3基因内CAG重复次数进行精确分析,并采用测序予以验证。
Methods : Fragment analysis with capillary electrophoresis was applied to count the CAG repeat of MJD gene and was proved with sequencing .
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尿白蛋白片段分析在糖尿病性肾病早期诊断中的意义
The analysis of urinary albumin fragments and its diagnostic significance in early diabetic nephropathy
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苹果砧木实生后代耐盐性鉴定的分子标记筛选及相关基因片段分析
Screen of Molecular Markers of the Salt-tolerance and Analysis of Related Gene Fragments in Apple Rootstock Seedling Progenies
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论非语言语境对翻译的影响&《红楼梦》译文片段分析
Influence of Non-Linguistic Context on the Translation & Analysis of pieces of translation from A Dream of Red Mansions
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经酶切和测序对插入片段进行分析、鉴定。经限制性内切酶和DNA测序分析两个基因是否已经正确连接到真核表达载体中。
The sequence of fusion gene was examined by enzyme incision and DNA sequencing .
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3种白鲑线粒体细胞色素b和16SRRNA基因片段序列分析
Sequence analysis of partial cytochrome b and 16S rRNA genes of three Coregonus species
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SARS冠状病毒基因片段变异分析
Analysis of Variations in Gene Fragments of SARS-CoV
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家蚕、野桑蚕线粒体cytb基因片段序列分析及分子进化研究
Analysis of Segment Sequences and Molecular Evolution Between Bombyx mori and Bombyx mandarina Using Mitochondrial Cyt b Gene
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方法以健康足月新生儿(30例)为对照组,采用凋亡细胞计数和片段DNA分析技术观察早产儿(19例)T淋巴细胞凋亡特性;
Methods The susceptibility to apoptosis of T lymphocytes were determined in 30 full-term neonates and 19 preterm neonates by percentage calculation of apoptosis cell and DNA fragmentation analysis .
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T5代水稻变异株系的AFLP分析和特异片段序列分析
AFLP and Specific Fragment Sequence Analysis of T5 Generation Rice Variants
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甘蓝型油菜(Brassicanapus)BAN基因同源片段克隆分析及油菜花序浸泡法转基因研究
Cloning and Analysis of the BAN-Homologous DNA Fragment and the Primary Study of Floral-Dip Method in Oilseed Rape Brassica napus
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目的建立检测普马拉病毒的RT-PCR方法,确认吉林省存在普马拉病毒,并对病毒的基因片段进行分析。
Objective To establish RT-PCR method for Puumala virus ( PUUV ) detection in order to confirm existence of Puumala virus in Jilin province and analyze gene sequence of Puumala virus .
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从AOA皮肤cDNA中随机选择14个片段测序分析:得到一个基因片段,与青鳃鱼和河豚的酪氨酸酶基因对比同源性只有25%和26.5%。
Selecting 16 cDNA clone fragments from cDNA in AOA skin , one clone shares 25 % and 26.5 % identities with the tyrosinase gene of Medaka fish and Fugu fish ;
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取1ugRNh逆转录为cDNA,荧光定量PCR共扩增hUAT和内参照GAPDH目的片段,分析计算hUATmRNA相对表达量。
Ug mRNA was reverse transcripted to cDNA and hUAT and GAPDH were amplified by real-time fluorescent quantitative PCR . Then relative expression quantity of hUAT mRNA was analyzed .
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近年来,一些不依赖于转录因子活性的新型双杂交系统相继建立,如分离的泛素系统、蛋白质片段互补分析、阻遏物重构分析和SOS恢复系统等。
Some new type of two-hybrid systems , such as split-ubiquitin system , protein-fragment complementation assay , repressor reconstitution assay and SOS recruitment system , have been developed recently .
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用常规法抽提外周血白细胞DNA,用聚合酶链反应反向点杂交法(PCRRDB)扩增目的基因片段和分析其基因类型。
DNA was extracted from peripheral white blood cell with standard method ( phenol chloroform extract ) . Polymerase chain reaction reverse dot blot ( PCR RDB ) was used for targeted DNA amplification and gene mutation analysis .
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目的克隆日本血吸虫磷酸甘油酸激酶(SjPGK)编码基因片段,分析其核苷酸序列。
Objective To clone and sequence the partial gene of Schistosoma japonicum phosphoglycerate kinase ( SjPGK ) .
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水稻红莲型细胞质雄性不育系与保持系mtRNA差异显示和差别片段的分析
Differential Display of Rice mtRNA in HL Type Cytoplasm Male Sterility ( CMS ) Line and Maintainer Line and Analysis of Differential Fragments
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云南兰坪地区带绦虫mtCOXⅠ片段测定分析任何单位或者个人未经许可,不得私自携带枪支入境、境。
Determination of mtCOX ⅰ fragments from tapeworms in Lanping County , Yunnan Province Without permission , no unit or individual shall enter or leave the country with guns .
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最后,本文对低氧短程硝化系统中的氨氧化细菌的amoA基因片段进行分析。
Therefore , it was presumed that the process of low DO was more suitable to the anaerobic oxidation of ammonia and to perform partial nitrification . Finally , this paper analyes Low DO Partial Nitrification system of ammonium oxidation amoA gene segments of the bacteria .