海藻糖

MolecularProperties在海藻糖分子构象分析中的应用

Application of Molecular Properties to the conformational analysis of trehalose

海藻糖封闭液在ELISA体系中的应用

Application of trehalose as blocking reagent in ELISA

海藻糖对低温储存皮肤β-actin的mRNA转录水平及其蛋白表达的影响

Effect of trehalose on the protein synthesis and mRNA transcription of β - actin after cryopreserved human skins

恶臭假单胞菌S1产胞内海藻糖合成酶发酵培养基的优化

Study on optimization of fermentation conditions with Pseudomonas putida S1

但是昆虫中只有少数几个物种的海藻糖合成酶基因的cDNA序列得到了克隆，有关其功能的报道更是少数。

But only a few TPS cDNA clones were obtained from insects and the researches on its function are also minority .

用TLC对58株细菌酶法合成海藻糖的能力进行初筛和复筛，得到3株海藻糖产量较高的菌。

Strains of bacteria were screened by TLC for their ability of trehalose production by enzymatic method .

有关细菌和真菌的海藻糖合成酶的报道已经比较多，大量编码海藻糖合成酶的cDNA序列也得到了克隆。

There have been many reports about trehalose-6-phosphate synthase in fungi and bacteria , the cDNA sequences encoding TPS have also been cloned .

海藻糖（trehalose）是一种化学结构稳定、无毒副作用的非还原性双糖，具有独特的生物学特性。

Trehalose , a disaccharide with stable chemical structure , has no any toxicity .

选定菌株A、B、C、F和H进行生产性能的测定，包括最适培养温度与比生长速率的测定，并通过热休克培养与加盐培养，对各酵母菌株进行生物量与海藻糖积累试验。

The production capability of strain A , B , C , F , H was determined , including suitable culture temperature , specific growth rate , biomass and trehalose accumulation .

本研究探讨以海藻糖（trehalose）为添加剂的血小板悬液冷藏保存方法。

The study was aimed to explore the trehalose method for storing platelets in cold .

研究结果如下：猪精子在冷冻干燥过程中，海藻糖的保护效果明显优于EDTA。

Results show that : The protective effect of trehalose is better than EDTA for boar sperm in the freeze-drying process .

研究了当海藻糖、蔗糖、乳糖在愈伤组织生长速度达最大时，对愈伤组织中三种同工酶EST、SOD、AMY的影响。

The isoenzymes of calli which grew on the medium with trehalose , sucrose and lactose were studied when the growth velocity was biggest .

经统计分析，小鼠早期囊胚存活率海藻糖组显著高于蔗糖组（P＜0.05）；桑椹胚存活率两组之间差异不显著。

The survival rate of blastocyst treated with trehalose were significantly higher than that of those treated with sucrose ( P0.05 ); while there was no significant difference between two groups in the survival rate of morula . 3 .

海藻糖合酶基因工程菌诱导表达条件的研究致病性鸡大肠杆菌pilA和外膜蛋白C原核诱导表达条件的优化

Study on induction conditions of trehalose synthase genetic engineering bacteria Optimized Expression of pliA and ompC from Avian Pathogenic Escherichia coli

也有果聚糖、海藻糖和蔗糖等可溶性糖类；Lea蛋白在很多植物抵御干旱胁迫过程中也起到了重要作用。

Osmolytes , Lea proteins and soluble sugars play an important role in drought-resistance in forest trees .

并且通过实验发现菌株S1的海藻糖合成酶为胞内酶，酶活力为7.30u/g菌体。

The trehalose synthase of S1 was detected to be an intracellular enzyme with the activity of 7.30 u / g wet cells .

利用硫酸-蒽酮反应，定量检测载入到红细胞胞内的海藻糖，同时对胞外游离血红蛋白和负载后红细胞ATP水平进行评价。

The sulfuric-anthrone reaction was used to determine the intracellular trehalose concentration loaded by RBCs , meanwhile we evaluate extracellular free hemoglobin and ATP level of loaded RBCs .

选用非渗透性保护剂海藻糖（trehalose），起到稳定细胞膜和蛋白质结构的作用，减轻低温状态下细胞水肿。

Choose the impermeability protective agent-trehalose which plays an important role in stabilizing hepatocytes membrane and protein structure and abates cell swelling . 3 .

海藻糖预处理可以缓解高温对膜的伤害，表现为电解质外渗量、MDA含量降低，O2.-和H2O2的积累减少。

While trehalose pretreatment alleviated heat induced damage to membrane , which was indicative of the decrease in electrolyte leakage , MDA content and the generation of O2 . - and H2O2 .

此外，含多拷贝11-氨基酸的多肽与海藻糖在保护LDH酶活性上存在协同作用，且二者有着不同的保护机理。

In addition , the peptides consisted of multi-copies of11-amino acid protect LDH by acting with trehalose synergically , and the protection mechanisms of LEA and trehalose on LDH are different .

硝酸镧高效催化合成3,4-二氢嘧啶-2（1H）-（硫）酮蒽酮-硫酸法测定海藻糖含量显色条件的改进

Lanthanum Nitrate as An Efficient Catalyst for the Synthesis of 3,4-Dihydropyrimidine-2 ( 1H ) - ( thio ) ones Improvement of chromogenic conditions on trehalose determination by anthrone-sulphuric acid method

结果海藻糖负载效率与孵育时间（2h后）、温度（30~40℃）呈良好线性关系。

Results The loading efficiency was linear to the incubated time ( 2 hours later ) and incubated temperature ( range from 30 ℃ to 40 ℃), respectively .

一种船用淡水制冰/海水蓄冷装置本研究探讨以海藻糖（trehalose）为添加剂的血小板悬液冷藏保存方法。

The equipment used freshwater for making ice and seawater for storing cold The study was aimed to explore the trehalose method for storing platelets in cold .

经微波诱变后获得的高产菌株海藻糖的产量为17.01g／100g干酵母。

Through microwave mutation the highest yielding of strain was 17.01g / 100g dry yeast .

采用摇瓶发酵法研究了不同碳源、氮源对恶臭假单胞菌S1干重和胞内海藻糖合成酶酶活力的影响。

Influences of carbon and nitrogen sources on Pseudomonas putida S1 dry weight and on the activity of intracellular trehalose synthase , were studied in shaking flask scaled Fermentation .

目的研究人血小板冻干保存前负载海藻糖至细胞内过程中DMSO的作用，优化血小板负载缓冲液配方。

Objective To study the effect of DMSO on platelets protection during the period of loading trehalose before lyophilization , and to optimize the method of loading buffer .

T6P水平受一组海藻糖-6-磷酸合成酶（TPS）和海藻糖-6-磷酸磷酸酶（TPP）调节。

T6P levels are potentially modulated by a group of trehalose-6-phosphate synthase ( TPS ) and trehalose-6-phosphate phosphatase ( TPP ) homologues .

按照确定的筛选方法，从两极地区采集的土壤菌样83株和极地海洋菌20株中最终筛选到一株产海藻糖合成酶转化率最高的一株菌S1，转化率为75.2%。

Among 83 and 20 strain samples from polar soils and oceans , strain S1 was obtained for the highest trehalose conversion rate from maltose e of about 75.2 % .

受到Al~（3+）胁迫后，细胞内SOD、海藻糖等明显增加，可能具有增强细胞抗Al~（3+）毒害的作用。

The SOD activity and trehalose content in the yeast cells increased obviously under the Al ~ ( 3 + ) stress , which probably contributed to the enhancement of Al ~ ( 3 + ) tolerance in yeast .

本实验目的是研究海藻糖对微生物谷氨酰胺转胺酶（TGase）热稳定性的作用。

The purpose of this study was to investigate the effects of trehalose on the preservation of Transglutaminase in heat systems .