波谱学方法

探测和解析蛋白质三维结构的实验工具主要有X射线晶体衍射方法和核磁共振波谱学方法。

Experimentally , the three-dimensional protein structure can be analyzed by x-ray diffraction of protein crystals or nuclear magnetic resonance spectroscopy .

目的建立安全、特异的13碳核磁共振（13cNMR）波谱学方法，观察严重烧伤大鼠肝脏细胞糖原异生和回补反应等合成代谢功能的变化。

Objective To introduce a safe and specific approach of 13 C NMR spectroscopy and investigate the alterations in hepatic anabolism functions including pyruvate recycling , gluconeogenesis and anaplerosis in severely burned rats .

采用反复的硅胶柱层析、SephadexLH-20等分离纯化手段，对荔枝核有效部位进行分离。并运用波谱学方法对其进行结构鉴定。

The repeated Silica gel column chromatography and Sephadex LH-20 etc. methods were used to separate and purify the compounds , and the spectroscopy method was used to identify the structures of the chemical compounds .

磁共振成像和波谱学方法研究抑郁大鼠脑内整体形态学及前脑和下丘脑变化。

Magnetic resonance imaging and spectrum were used to study the changes of whole brain and frontal area and hypothalamus area separately .

另外相容性也可以通过波谱学方法加以辅助说明。以上预测和表征方法都与合金膜具体实例联系，表明这些方法在合金膜研究中有着广泛的应用。

Relating to many examples of alloy membranes , the predicting and characterization methods of alloy membranes have great application in the alloy membrane research .

用核磁共振谱、红外光谱、质谱等波谱学方法并结合理化性质确定了31个化合物的结构，其中包括3个新天然产物（1~3）。

Thirty-one compounds were identified on the basis of spectral methods ( 1D-NMR , 2D-NMR , MS , IR , etc. ) and their physicochemical properties . Among them , there were three new natural products ( 1 ~ 3 ) .

方法用硅胶柱层析进行分离，用有机波谱学的方法进行结构鉴定。

Methods Compounds were isolated by silica column chromatography , and their structures were elucidated by spectral methods .