汉坦病毒

青岛地区2000-2003年HFRS患者汉坦病毒部分M基因的检测及其分型研究

Analysis of part of M gene and genotyping for M segment of hantavirus detected from HFRS patients'sera in Qingdao region during 2000-2003

方法：采集2000~2001年肾综合征出血热（HFRS）患者的血液标本，通过逆转录聚合酶链反应（RT-PCR）扩增汉坦病毒M片断基因。

Methods : The blood samples were collected from HFRS patients between 2000 ~ 2001 , and the M segment genome of hantavirus was amplified by RT-PCR .

逆转录-套式PCR检测汉坦病毒感染的实验研究

Detection of hantavirus infection using reverse transcriptional-nested polymerase chain reaction

汉坦病毒沟3株PCR分型

PCR Typing of Hantavirus Gou 3 Strain

汉坦病毒S片段荧光定量PCR参考标准品的构建

Construction of reference standard plasmids of real-time fluorescence quantitative PCR for detecting Hantavirus S segment

3从阳性鼠肺中提取汉坦病毒RNA，逆转录合成cDNA；

RNA of HV was extracted from positive lungs and cDNA was synthesized ;

RT-PCR定量检测汉坦病毒RNA及在临床诊断中的价值

Quantitative determination of Hantavirus mRNA with RT-PCR and its value in clinical diagnosis

应用反转录-套式PCR方法检测肾综合征出血热病人血清中的汉坦病毒特异性RNA

Hantavirus RNA detection in clinical sera of hemorrhagic fever with renal syndrome using reverse transcription-nested PCR

逆转录-半套式聚合酶链反应检测汉坦病毒RNA及其临床初步应用

Detection of Hantavirus by reverse transcriptase semi nested polymerase chain reaction and its preliminary application

RT－PCR，ELISA和IFA技术应用于汉坦病毒检测的比较

Comparison of RT-PCR , ELISA and IFA for detecting Hantavirus

[目的]构建汉坦病毒S片段标准品，用于实时荧光定量PCR检测汉坦病毒。

[ Objective ] To explore the method of preparation for Hantavirus S segment standard plasmids of real-time PCR .

一株自褐家鼠分离的汉坦病毒SD（10）株分子生物学特征研究

The Molecular Biological Characteristics of the Hantavirus Strain SD_ ( 10 ) Isolated Rattas Norvegicus

以汉坦病毒重组核衣壳蛋白为抗原进行ELISA血清分型的研究

Use of recombinant nucleocapsid protein of hantavirus as antigen in ELISA for serotyping sera from HFRS patients

抗汉坦病毒单克隆抗体F（ab'）2的制备及其纯度和活性鉴定

Preparation and immunological characterization of the F ( ab ′) 2 fragments of two monoclonal antibodies against Hantaan virus

用聚合酶链反应检测肾综合征出血热患者血、尿中汉坦病毒RNA

Detection of hantavirus RNA in blood and urine in hemorrhagic fever with renal syndrome by polymerase chain reaction

目的比较利巴韦林等五种药物对汉坦病毒（HV）的体外抑制作用。

Objective To compare the antiviral activity of five drugs in vitro .

汉坦病毒基因组G1区部分酶切位点的研究

Study on the Endonuclease Site of G1 Region of Hantavirus Genome

抗汉坦病毒NPscFv基因表达及生物活性分析

Gene Expression and Bio-Activity Analysis of scFv of Hantavirus NP

汉坦病毒湖北株M片段G1编码区基因变异研究

Genetic Variation of Glycoprotein G1 Encoding Region of Hantaan Virus Hubei Strain

原癌基因fos在汉坦病毒感染神经元内的表达

Expression of Fos in neuron of Hantaan virus infection

汉坦病毒全NP蛋白及其型特异区编码基因的克隆及表达

Clone and Expression of Total NP and NP Type Specific Region Coding Gene of SEO Virus

结论吉林省褐家鼠主要携带S3亚型的汉城型汉坦病毒。

Conclusion The subtype of Seoul hantaviruses in Jilin province was S3 .

天津地区汉坦病毒的分子流行病学分析及其GP多表位抗原基因的构建与表达的研究

Studies on Molecular Epidemiology of Hantavirus in Tianjin and GP Multi-epitope Antigen Gene Construction and Expression

PCR-SSCP法分析汉坦病毒在乳鼠中的基因变异

Analysis of Hantavirus genetic variations in suckling mouse with PCR SSCP method

表明近年福建省流行的汉坦病毒仍以SEO型为主。

It shows that Seoul virus is a predominant serotype in fujian province .

北京地区流行性出血热SEO型汉坦病毒基因差异的研究

Study on the genetic difference of SEO type Hantaviruses

汉坦病毒Vero细胞灭活疫苗候选毒种筛选

Selection of virus strain for production of inactivated hantavirus vaccine of vero cells

汉坦病毒核蛋白抗原（NP）在人体液免疫反应中起重要作用，据此本研究针对上述需求发展了由单链抗体（scFv）介导的免疫荧光蛋白质芯片和免疫PCR检测方法。

According to the requirements above , we develop several immunoassay methods : mainly scFv-mediated immunofluorescence protein chips and immuno-PCR .

汉坦病毒A9株M基因片段全长cDNA克隆及其在痘苗病毒中的瞬时表达

Cloning and Transient Expression of M Genome Segment cDNA of Hantaan Virus Chinese Isolate A9

插入CpG序列的汉坦病毒S基因真核表达载体的构建及表达

Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif