核酸酶

气升式发酵罐生产核酸酶P1发酵过程的动力学

Study on kinetics of nuclease P_1 fermentation in airlift tower loop reactor

固定化桔青霉发酵核酸酶P1的研究青春野绿

The Green Banana Study on nuclease P_1 produced by immobilized Penicilium citrinum cell

桔青霉生产核酸酶P1发酵条件优化研究

Study on the Optimization of Fermentation Conditions for Penicillium citrinum Producing Nuclease P_1

核酸酶P1固定化研究

Studies on the Immobilization of Nuclease P_1

在金黄色葡萄球菌核酸酶测活体系中Ca~（2+）与底物DNA的结合

The binding of substrate DNA and calcium in staphylococcal nuclease assay system

固定化桔青霉气升式反应器生产核酸酶P1的研究

Study on Production of Nuclease P_1 by Immobilized Penicilium Citrinum Cell in Three Phase Airlift Reactor

胞外核酸酶对于调节胞外DNA分子起到重要的作用。

Extracellular nuclease plays an important role in regulating the amount of extracellular DNA molecules .

本研究对于遗传工程中的化学核酸酶以及以DNA为靶标的药物设计有重要的意义。

These studies are very important to the design of chemical nucleases and DNA-targeting drugs .

核酸酶P1催化水解热变性DNA的研究

Studies on enzymatic hydrolysis of the heat-denatured DNA by nuclease P1

金属离子对核酸酶P1催化水解RNA反应的影响及其机理探讨

The effect of metal ions on catalytic hydrolysis of RNA by ribonucleic acid enzyme p_1 and its mechanism

通过将几个锌指出串联在一起，再加入一个切割DNA的核酸酶，研究人员们能够精确地导向将被切割的特异性基因。

Bystringing together several zinc fingers and adding a DNA-cleaving nuclease , researchers can precisely target specific genes to be cut .

人工核酸酶是一类具有限制性内切酶的功能、能高效高选择性地催化水解DNA或RNA的断裂工具。

Artificial nucleases are compounds that cleave nucleic acids and function as natural restriction enzymes with high activity and selectivity .

能够对DNA或RNA进行序列特异性切割的化学试剂，是一类非酶裂解新工具，被称为化学核酸酶或人工核酸酶。

Chemical nuclease or artificial nuclease is a novel cleavage tool , which can cleave the DNA or RNA sequence specifically .

由于金属核酸酶小分子对DNA具有较好的切割性，越来越多的人们开始关注和设计此类核酸酶小分子。

Because of the highly cutting of metal nuclease to DNA , more and more people begin to pay attention and design these nuclease moleculars .

方法：应用核酸酶保护法检测系膜细胞肿瘤坏死因子α（TNF-α）、IL-1α和IL-1βmRNA表达；

Methods : TNF-a , IL-la and IL-lp mRNA expression was determined by ribonuclease protection assay .

核酸酶BN及SN基因的克隆和序列分析

Cloning and sequencing of the nuclease BN and Sn gene

刺槐核酸酶Ⅰ的分子量为44000，最适pH为6.2，最适温度为70℃。

The molecular weight of the nuclease I was 44000 . The enzyme gave pH optima of 6.2 and temperature optima of 70 ℃ .

在体外，La蛋白能够与HBvRNA结合，保护HBvRNA免受核酸酶的破坏。

Recently La protein was identified in vitro as HBV RNA-binding protein and it can protect HBV from the nuclease .

目的建立金黄色葡萄球菌核酸酶（SNase）去C－末端的肽段52（SN52）和79（SN79）的提纯方法，并鉴定其纯度。

Objective To establish method for separation and purification of staphylococcal nuclease peptide fragments 52 and 79 with removed carboxy terminal end ( SN 52 , SN 79 ) .

He-Ne激光和UV-B辐射对小麦幼苗核酸酶的影响

Study on the Nuclease of Wheat Seedling under He-Ne Laser and Ultraviolet-B Radiation

讨论了核酸酶保护法在CMV株系鉴定中的作用。

The applicationof ribonuclease protection assay was discussed in the identification of CMV strains .

常规分子信标在活细胞内mRNA表达水平的研究中，由于细胞内核酸酶对分子信标骨架的降解和破坏作用，常导致假阳性信号的产生，对检测结果的准确性影响较大。

The normal molecular beacons tend to generate false positive signals due to nuclease degradation , when used for intracellular mRNA expression level analysis .

目前正对HIV感染者进行锌指核酸酶临床实验，利用这种酶删除一段使病毒进入免疫细胞的基因。

Clinical trials are underway in HIV patients of zinc-finger nucleases that remove a gene that allows the virus to enter immune cells .

运用核酸酶保护试验观察RB基因的点突变现象。

Applying RNase Protection assay , the point mutation in the RB gene was observed .

锌指核酸酶是一种人工制做限制性内切酶，通过将锌指DNA结合区与限制性内切酶的DNA切割区融合获得。

Zinc finger nucleases ( ZFNs ) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme .

可见光谱法研究核酸酶P1与氯化铜（Ⅱ）的相互作用

Study on the Interaction between Nuclease P1 and CuCl_2 (ⅱ) by Visible Absorption Spectra

S1核酸酶突变检测法的优化

Modification of the S1 nuclease Mutation Detection

然而，由于目前在实验上很多锌指核酸酶的晶体结构很难得到，这样就限制了锌指核酸酶和DNA相互作用的理论研究。

However , since crystal structure of the zinc finger nuclease has not been obtained in experiments , theoretical studies on interactions of zinc finger nuclease with DNA remain very limited .

用S1核酸酶图谱法测得转录从rnc基因伸延到era基因。

Extending of the transcription from rnc to era has been proved by means of S1 nuclease mapping .

基于分子信标和S1核酸酶发展了一种检测锌离子的新方法。

A new method for zinc detection based on molecular beacon and S1 nuclease was developed .