核酸内切酶

核酸内切酶在DNA断裂损伤检测中的应用研究

Application of Endonuclease to Detecting the Strand Breaks of DNA Oxidative Damage

限制性核酸内切酶与DNA相互作用研究进展

Progress on the Interaction of Restriction Endonuclease and DNA

补充核酸内切酶Ⅲ对DNA氧化损伤影响的研究

The effect of endonuclease ⅲ supplementation on the damage of DNA strand breaks

人乳头瘤病毒DNA的限制性核酸内切酶分析

Restriction enzyme analysis of human papilloma virus ( hpv ) dna from common warts

该法提取的血吸虫基因组DNA较纯，适合于以后进行DNA限制性核酸内切酶谱分析和Southernblot分析。

The technique is useful in the analysis of restriction endonuclease digestion of genomic DNA and Southern blot .

本文应用限制性核酸内切酶HaeⅢ显示了孟氏裂头绦虫染色体G分带，获得了较好的G带带纹。

G-banding of the chromosomes meets with success by using restriction endo-nucleas Hae ⅲ in Spirometra mansoni .

T4核酸内切酶V的分离纯化

Isolation and Purification of T4 Endonuclease V

HSV临床分离株的限制性核酸内切酶酶切分析与鉴定

Restriction endonuclease cleavage analysis of DNAs from herpes simplex verus clinical isolates

方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。

Methods : 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction ( PCR ) technique .

结果：PCR和限制性核酸内切酶图谱分析均证实所获重组质粒中含有P1蛋白基因。

Results : Recombinant plasmid was proved to carry P1 protein gene by using PCR and endonuclease map analysis method .

限制性核酸内切酶PstⅠ活性基团的研究

Studies on the Active Groups of Pst I Restriction Endonuclease

方法应用PCR技术扩增T细胞受体γ链基因重排，选用四种限制性核酸内切酶消化扩增产物。

Methods After amplifying T-cell receptor γ chain gene rearrangement using polymerase chain reaction ( PCR ), we digested the products with 4 kinds of restriction enzyme .

目的研究人胃黏膜上皮细胞系无嘌呤无嘧啶核酸内切酶（APE）的表达状况。

Objective To study the expression of APE / Ref-1 in gastric mucosal epithelial cell lines .

Ⅱ型限制性核酸内切酶NcrⅠ的研究

Study on A Type ⅱ Restriction Endonuclease Ncr ⅰ

Serratiamarcescens非特异性核酸内切酶的原核表达及其应用

Prokaryotic Expression and Application of Serratia marcescens Non-specific Endonuclease

结果：（1）通过DNA测序和限制性核酸内切酶酶切鉴定证实，hMMP-12成功克隆到所需表达载体中；

Results : ( 1 ) The hMMP-12 was successfully cloned into the needed vector by enzymatic digestion and DNA sequencing ;

以DNA分子与各种生化酶（Nicking核酸内切酶、核酸外切酶、聚合酶）间的生化反应来模拟数据的读取和写入。

Biochemical reactions between DNA memory and enzymes ( Nicking Endonuclease , Exonuclease and Polymerase ) can be defined as reading and writing operations .

质粒pBR322的体外扩建以及有关限制性核酸内切酶图谱

In vitro Enlargement of pBR322 and the Related Restriction Enzyme Maps

通过PCR扩增狂犬病病毒SAD-B19株的G、N基因。将回收的目的片段用限制性核酸内切酶PstI进行酶切。

The G and N gene of rabies virus SAD-B19 strain were amplified by PCR , the target genes were collected and digested by Confine digestion enzyme of PstI .

在没有核酸内切酶存在时，QDs的荧光通过能量转移被猝灭，纳米探针处于关闭状态。

In the absence of endonucleases , the fluorescence of QDs is quenched through energy transfer effect , and the nanoprobe stays in the " OFF " state .

目的：观察脑缺血后半胱氨酸蛋白酶3（caspase-3）和脱嘌呤/脱嘧啶核酸内切酶（APE/Ref-1）的表达，探讨脑缺血损伤与修复机制。

AIM : To explore the mechanism of neuronal injury and repair by investigating the expression of caspase-3 and apurinic / apyrimidinic endonuclease ( APE / Ref-1 ) after focal cerebral ischemia .

目的氧化/还原因子1（ref1），也称为AP核酸内切酶（APE），是氧化还原和DNA碱基切除修复途径中的重要一员。

Objective Redox factor 1 ( ref-1 ), also known as AP endonuclease ( APE ), is one of the very important components of DNA base excision repair and redox .

目的探讨DNA损伤修复基因脱嘌呤/脱嘧啶核酸内切酶（APE1）在鼻腔NK/T淋巴瘤中的表达及其临床意义。

Objective To investigate the expression of DNA damage and repair gene Apurinic / apyrimidinic endonuclease ( APE1 ) protein in nasal NK / T cell lymphoma , and elucidate its clinical implication .

T7核酸内切酶I（T7EndonucleaseI，T7EndoI）是一种研究非常深入的多功能核酸内切酶，由大肠杆菌T7噬菌体基因3编码。

T7 Endonuclease I ( T7 Endo I ) is a very in-depth studied multi-functional endonuclease , which is coded by Escherichia coli T7 phage gene 3 .

用X-gal平板及质粒图谱分析方法筛选重组克隆株，再用限制性核酸内切酶酶切图谱分析鉴定。

The recombinant clone was screened by X-gal plate and plasmid map , and identified by restricted enzyme mapping analysis method .

方法应用聚合酶链反应、DNA测序和限制性核酸内切酶酶切等技术对15个AR-JP家系先证者的parkin基因进行突变研究。

Methods the polymerase chain reaction ( PCR ), DNA sequence analysis , and restriction enzyme digestion analysis were applied to check parkin gene mutations of 15 index patients from 15 families with AR-JP .

由此可见，抗APO－1单抗与其特异性抗体结合后可能是通过使胞浆中游离Ca2＋浓度升高，激活了Ca2＋／Mg2＋依赖的内源性核酸内切酶而导致SEB活化的淋巴细胞凋亡。

It suggests that the Ca 2 + / Mg 2 + depended nucleic acid endonuclease in lymphocytes treated with SEB is activated when APO 1 receptors on cells interact with its specific antibody and induce activated lymphocytes apoptosis finally .

其特点在于结合使用限制性核酸内切酶EcorI及纳米金标记的检测探针，能够很好的降低背景值并有效增强信号。

It features combined with restriction endonuclease ECoR I and nano-gold-labeled detection probe that can reduce the background of a very good value and effective way to boost the signal .

GzmA靶向作用于一种与内质网结合的特殊的复合体&SET复合体，其包含3种GzmA底物：核小体装配蛋白SET、DNA结合蛋白HMG-2、具有碱基切除修复作用的核酸内切酶Apel。

A special target of the granzyme A cell death pathway is an endoplasmic reticulum-associated complex , called the SET complex , which contains three granzyme A substrates , the nucleosome assembly protein SET , the DNA-binding protein HMG-2 , and the base excision repair endonuclease Apel .

利用限制性核酸内切酶PstⅠ在不同的有机介质中的催化反应，研究了水含量、相对粘度，介电常数和渗透压等介质的物化性质对PstⅠ原活性和星号活性的影响。

Through the catalyzed reactions of the restriction endonuclease Pst ⅰ in different organic medium , the effect of the four physico-chemical properties such as total water content , relative viscosity , dielectric constant and osmotic pressure on the prime and the star activity of Pst ⅰ was studied .