全酶

HIV-1整合酶全酶结构模拟及其抑制剂筛选

The Structural Investigation of the HIV-1 Integrase Holoenzyme and the Screening of Its Inhibitors

细菌可以利用选择性σ因子引导RNA聚合酶全酶结合到特殊类别的启动子上来调控基因的表达，从而使细菌适应环境的变化。

Bacteria can use alternative sigma factors , which direct the RNA polymerase holoenzyme to a specific class of promoters , to adapt to environmental changes .

全酶还能识别DNA双链分子上的特定核苷酸序列（起动基因）。

The holoenzyme recognizes specific nucleotide sequences on the DNA duplex ( promoters ) .

并应用定量细胞化学法对LDH和6PD全酶活性进行了测定。

Total G6PD and LDH values were assayed cytochemical methods .

这些负控蛋白可结合CDK而抑制全酶活性，影响DNA复制从而抑制细胞增生。

They combined with CDK and inhibited their activity , affect DNA copy to restrain cell proliferation .

由SDS-PAGE和梯度PAGE测得全酶由4个亚基组成，全酶相对分子质量为360000，亚基相对分子质量为100000。

The molecular weight of the subunit and the whole enzyme were estimated by SDS-PAGE and gradation PAGE as 100 000 and 360 000 , respectively .

通过RT-PCR方法扩增出CPA1全酶及活性中心基因，分别克隆入载体pGEM-Teasy，测序分析。

The carboxypeptidase A1 and the active center gene were amplified by RT-PCR from pancreas tissue and cloned into vector pGEM-T easy .

目前蛋白质数据库（ProteinDataBank，PDB）中有26个关于HIV-1整合酶的晶体结构，但仅仅是包含整合酶的单个结构域或其中的两个结构域，无整合酶全酶的晶体结构。

At present , there are 26 crystal structures of HIV-1 integrase in the protein data bank , which include single domain or two domains of it , but none of them is the holoenzyme structure of HIV-1 integrase .

经SDSpage分析，全酶由45.6KD的小分子的ADA亚单位和无ADA活性的结合蛋白（ADAbp，其分子量为68.1KD和108.7KD）构成。

The purified ADA molecule consists of 45.6 KD Mr small subunit ADA 、 inactive 68.1 KD Mr and 108.7 KD Mr binding protein ( ADAbp ) on a 10 % SDS_PAGE .

方法：利用全酶法对112例正常成人血浆中1,5-AG水平进行测定。

Methods : To measure the plasma 1,5-AG of 112 healthy volunteers by fully enzymatic method .

周期素D1编码一种全酶的调节亚单位，该酶利用蛋白磷酸化灭活视网膜母细胞瘤蛋白，通过细胞周期G1-S期促进肿瘤进展恶化。

Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle .

由此说明，水芦、沙芦Rubisco全酶蛋白及其大亚基免疫学特性差异较小，而与双子叶植物菠菜相比差异较大；

It follows that Rubisco holoenzymes and their small and large subunits of swamp reed and dune reed slightly differed in their immunological characteristics but differed greatly from those of dicotyledonous spinach ;

然而，仅仅通过分步透析除去变性剂，Rubisco的亚基仍然不能重组成全酶，除非再加入分子陪伴蛋白60（cpn60）。

However , fully denatured Rubisco subunits still could not be reassembled into holoenzyme after gradual dialysis unless chaperonin 60 ( cpn60 ) was added .

活性分析显示CPA1全酶和活性中心蛋白都具有一定的催化水解活性，但后者对肿瘤细胞的杀伤效果较弱。

Both of the expressed proteins have catalytic activity in vitro , but the activity of the latter is inferior when applied to tumor cells .

pH4.0时测定到全酶分子的总色氨酸残基数为12，用邹氏图解法求得其中2个色氨酸残基为表现活性所必需。

The total number of 12 tryptophan residues per enzyme molecule were determined at pH 4.0 , but two of them are essential for enzyme activity as measured by Tsou 's graphical method .

马铃薯淀粉磷酸寡糖的全酶法制备及其分离

Study on the Enzymatic Preparation and Separation of Potato Phosphoryl Oligosaccharides

以碎米为原料，采用全酶法制备超高麦芽糖浆。

Extremely high maltose syrup was enzymatically produced from broken rice .

全酶法测定血浆1,5-脱水葡萄糖醇在全自动生化分析仪上的应用

The measurement of plasma 1,5-AG by fully enzymatic method on automatic analyzer

一种新的全酶法测定1,5-脱水葡萄糖醇

A new full enzymatic method for measuring 1,5 - anhydro - D-glucitol

红麻全酶法脱胶工艺及机理研究

Processing and Mechanism of Enzyme Degumming of Jute / Kenaf

全酶法液态深层发酵制醋生产线的建立和控制

Establishment and control of vinegar production line for submerged fermentation by enzymes

全酶法制备大麦糖浆的研究

Study on Preparation of Barley Syrup with Enzyme

全酶法生产高麦芽糖浆及其工艺研究

Enzymatic Technology for Producing High Malt Syrup

超氧化物歧化酶全酶和脱辅基酶胍变性时失活与去折叠的比较研究

A Comparison of Inactivation and Unfolding of Holo and Apo Superoxide Dismutase during Guanidine Denaturation

对以大米为原料，采用全酶法同时试制超高麦芽糖浆和高蛋白米粉进行了研究。

A new technology for the separation and cleanse of high maltose syrup with adsorption resins was proposed and studied .

目的筛选、鉴定新的人端粒相关蛋白，为进一步了解端粒酶全酶的结构、生物学功能及其作用机制奠定实验基础。

Objective Identifying telomere associated proteins in order to make experimental basis for understanding the structure of telomerase holoenzyme and its biological function .

为探讨镍离子对脲酶结构和功能的影响，通过构建乙酰氧肟酸抑制脲酶的全酶和脱辅基酶体系进行分子动力学模拟。

To study the role of nickel ions on urease structure and function , acetohydroxamic acid inhibited urease and apo enzyme was modeled by molecular dynamics simulation .

研究了以国产大麦为原料，采用全酶法经预水解、液化和两段糖化制备大麦汁的生产工艺。

It was researched on the new preparation process of barley wort through partial hydrolysis , liquefaction , two-steps saccharification by enzymatic method , used domestic barley as raw material .

但由于HIV-1IN的全酶结构未知以及受体外筛选实验的限制，使得基于受体结构的药物设计进展缓慢，目前仅有一个化合物作为整合酶抑制剂用于临床治疗。

However , lack of complete IN structure and the limitation of its screening method for inhibitors in vitro , blocked the development of drug design based on receptor . So far , only one compound as integrase inhibitors has been used in clinical treatment .

研究表明，以不适用于发芽的大麦与脱脂玉米粉为原料，全酶法可生产出与麦汁成分相近的大麦糖浆，具有丰富的α-氨基氮等酵母营养物质。

Results showed that using barely , which was not suitable for germination , and de-fatted corn powder as materials , barely syrup which was similar to malt juice in composition could be produced and it contained plenty of α - amino nitrogen and other nutrient .