杂细胞

采用L9（34）正交试验法，对影响杂细胞沼气发酵的基质浓度、发酵温度、添加剂三因素进行了初步研究。

The initial study was conducted on the mutual relationship of the factors as substrate concentration , additive and temperature by means of the L9 ( 34 ) orthorhombic experimental method .

纤维长度和杂细胞的含量对甘蔗渣浆脆性影响的初步研究

Preliminary Studies on the Impacts of Fiber Length and Non-Fibrous Cell Contents on Bagasse Pulp Brittleness

纤维平均长度约为1.0mm，长度分布较为均匀，杂细胞含量相对较少。

The fiber length of the palm thread was relatively short with fiber average length of about 1.0 mm , however fiber length distribution was relatively uniform . The non-fiber cell content was relatively low .

麦草化学浆含有杂细胞及细小纤维组分，这些组分在一定程度上和机械木浆的细小纤维组分相似，但麦草浆的主要性质的发展规律主要和化学木浆相似。

Non-fiber elements and fines existing in chemical wheat straw pulps , though to a certain extent much similar to the fine fractions in mechanical wood pulp , behave more closely to those found in chemical wood pulps .

方法：1.解剖显微镜下，无菌操作分离获取豚鼠耳蜗血管纹组织，经Ⅰ型胶原酶消化后接种培养，反复多次刮除杂细胞并使用内皮细胞条件培养液对所培养的内皮细胞进行纯化。

Methods : 1 . Aseptically , the cochlear stria vascularis was acquired from normal guinea pigs with micradissection and the endothelial cells were isolated by Collagenase ⅰ digestion . Then , they were cultured and purified by using conditioned medium and mechanical curettement .

目的：文献报道用胶原酶非灌注消化离体大鼠肝脏，其所得肝细胞数量少，活性较差且有大量的杂细胞污染，给进一步的研究造成了难以克服的影响。

Objective : Lots of literatures had reported the methods that digested the liver from mammals through non-perfusion for hepatocyte preparation , but hepatocyte viability and yield obtained by above methods were not satisfactory , moreover , contaminated with lots of other cells so that further research was hampered .

结论：所检测的PCR引物对之间的扩增效率一致，可用于比较分析mbr+/mbr-Nalm-6杂合子细胞系中两个等位基因表达活性。

Conclusion : Amplification efficiency of primers in real-time PCR reactions is identical . These primers can be used for quantity analysis of transcription activity of two BCL2 alleles in mbr + / mbr-Nalm-6 heterozygous cell line .

方法用DRB基因类引物扩增该特殊的DRB1杂合子细胞基因组的DRB基因的第二外显子。扩增产物经纯化转染大肠杆菌JM101，克隆后再经酚／氯仿抽提后得到单链M13DNA。

Methods Amplification of exon 2 of DRB gene in this heterozygote was carried out with genomic DNA templet and generic primers and the amplicon was cloned in M13 for further sequencing .

牛视网膜提取物对人-鼠杂合细胞抗体产生的影响

Effects of Bovine Retinal Extract on Production of Human IgM in the Culture of Human-Mouse Hybridoma Cells

我们认为，胚胎干细胞不能完全对肿瘤细胞进行重新编程，所得到的杂合细胞是一种具有和胚胎干细胞相类似的可以无限增殖和自我分化的畸胎瘤细胞。

We conclude that ES cells cannot completely reprogram tumor cells to become the hybrid cells ( 4N ) that are similar to ES cells ( 2N ) . What we have got were some kind of ES cell like teratoma with infinite self-renew and differentiation ability .

Na~+-K~+-ATP酶抑制引起的细胞凋亡和杂合性细胞死亡

The Apoptosis and Hybrid Cell Death Induced by the Na ~ + - K ~ + - ATPase Blocking

结论：双重杂合子红细胞存在两种遗传缺陷，易互相干扰诊断提示。

Conclusion : The diagnostic clues of double heterozygote could be interferenced by two kinds of abnormalities in erythrocyte .

5氮杂胞苷诱导NB细胞Caspase8表达及对TRAIL敏感性的研究

Induction of Caspase 8 by 5-Azacytidine renders NB cells sensitive to TRAIL

－1700～－161bp顺序中含有增强CPSⅠ启动子转录作用的顺序。地塞米松和促分化剂硫杂脯氨酸对肝癌细胞中CPSⅠ的转录均有改善作用。

Dexamethasone and thioproline ( a differentiation inducer ) showed enhancing effects on CPS ⅰ gene transcription in hepatoma cells .

材料与方法不同浓度的5-氮杂胞苷与Bcap-37细胞共培养后，应用细胞计数法检测Bcap-37细胞生长的情况；

MATERIAL AND METHODS : After treatment of Bcap-37 cells with 5-azacytidine , cell proliferation was analyzed by cell counting .

12例杂合性肝细胞癌组织标本中，有10例p73基因存在G/C：A/T双等位基因表达，在癌旁组织中则均为G/C表达，未见A/T表达。

Allele-specific analysis showed that the expression of G / C : A / T was present in 10 of 12 heterozygotic hepatoma specimens , whereas in the para-cancerous specimens only expression of G / C was present .

正常人的标准带型分布：在检测的110名正常人中，74.5%（82／110）为标准带型，无易位、杂合、体细胞嵌合等情况。

The standard pattern distribution of normal population : Most ( 74.5 % , 82 / 110 ) of the unrelated healthy individuals displayed such a pattern without any translocation , hybridization and mosaicism .