杂交探针

这种方法需要通过PCR扩增获取目的基因的一段序列或通过测定植酸酶蛋白的部分氨基酸序列来设计杂交探针，也有通过表达来筛选基因文库的报道。

A hybridization probe is usually designed either by amplifying a fragment of the target gene or by determining partial amino acid sequence of the protein .

方法利用荧光共振能量转移的原理，设计检测人葡萄糖6磷酸脱氢酶（G6PD）基因的荧光杂交探针，在熔解曲线模式下，设定不同模板量、Mg2+浓度，观察熔解曲线峰面积及其Tm值。

Methods A pair of G6PD specific fluorescein labeled oligonucleotide hybridization probe were designed according to the principles of fluorescence resonance energy transfer . The melting curve peak area and melting temperature were observed at different concentrations of template and Mg 2 + for the mode of melting curves .

本文叙述一种将辣根过氧化物酶（HRP）与单链DNA交联以制备非同位素核酸杂交探针的新方法。

A new method for constructing nonradioactive hybridization probes was studied , which allowed crosslinks to be producedbetween HRP and single-stranded DNA .

植物叶绿体4.5S核糖体rRNA作为分子杂交探针的研究

Studies on chloroplast 4.5s rRNA as common hybridization probes

8份PCR和血培养均阳性的标本，基因芯片杂交探针阳性菌株与血培养细菌阳性结果完全一致。

Two were positive by Bacillus and Propionibacterium probes , separately ; 4 were positive by CoNS . The 8 specimens which showed positive results by both PCR and blood culture , the result of gene chip hybridization coincided with the result of blood culture .

Polymin-HRP核酸杂交探针

A polymin-hrp labeled probe for DNA hybridization

荧光杂交探针技术检测单核苷酸多态性

System for Detecting Single Nucleotide Polymorphisms Based on Hybridization Probes

克隆化反向杂交探针的制备

Preparation of Cloned Probes for Reverse Dot Blot

国产荧光原位杂交探针在产前染色体异常诊断中的应用研究

Evaluation of domestic fluorescence in situ hybridization probes in prenatal diagnosis of chromosome abnormality

目的建立一种基于荧光杂交探针技术快速检测单核苷酸多态性方法。

Objective To establish a system for rapidly identifying single nucleotide polymorphisms based on hybridization probes .

双色双融合间期荧光原位杂交探针检测慢性髓系白血病的微小残留病状态

The application of dual fusion interphase fluorescence in situ hybridization probe in detecting minimal residual disease in chronic myeloid leukemia

应用合成的寡核苷酸作杂交探针来检测β地中海贫血基因，在3个患病家庭中鉴定出5种不同的点突变型。

The synthetic oligonucleotides were used as hybridization probes to detect the β thalassemia genes , and 5 different point mutations in 3 affected families were identified .

研究的主要结果如下：1.使用已有的61078组大豆杂交探针对花生抗、感两品种进行杂交。

The main results of research as follows : 1.The existing 61078 group of soybean hybridization probes were used to hybridise resistant and susceptible varieties of peanut .

同时，验证了选择重复序列含量较少的玉米BAC作为FISH杂交的探针也是获得特异性杂交信号的重要条件。

The results demonstrated a important factor of receiving specific signals of BAC-FISH by select maize BAC-probes for lesser content repeat sequences .

信使RNA是传递DNA遗传信息的工具，它也可以通过逆转录酶的催化合成互补DNA（cDNA），用作分子杂交的探针，以分离特定的基因。

The mRNA is a tool transferred genetic information for DNA and can be copied by reverse transcriptase to form a cDNA which can be used as a probe for molecular hybridization to isolate specific genes .

基因组原位杂交中探针长度的优化

Optimization of Probe Length in Genomic in Situ Hybridization

用磁珠回收特异性杂交的探针，经生物素标记的通用引物扩增后，与相应的寡核苷酸微阵列芯片杂交。

The selective probes after MAPH were collected with streptavidin coated magnetic beads and amplified by a biotin labeled universal primers .

点杂交方法检测探针标记效果。

Effect of labeling was detected by dot blot hybridization .

在杂交过程中探针混合液的配比上去掉了硫酸葡聚糖，两种不同标记的探针混合比例为1：1，其他组分浓度不做更改。

During FISH , the dextran sulfate was removed in the hybridization mixture in which the proportion of the two probes with different tag was 1:1 .

PCR技术较体外杂交技术和DNA探针有更高的敏感性。

Compared with biotinylated DNA probes and hybridization technique , PCR has a higher sensitivity .

用NorthernBlot杂交分析法（探针为地高锌标记的POMCcRNA），观察垂体POMCmRNA表达水平。

Pituitary POMC mRNA expression was analyzed by Northern blot analysis ( using Dig labeled POMC cRNA probe ) .

结果聚合酶链反应分别扩增出263、351、370bp三种DNA探针，肺炎链球菌和流感嗜血杆菌只与其对应的探针杂交，细菌通用探针和病毒、真菌间无交叉反应。

Results The DNA probes of 263 , 351 , and 370 bp were amplified by PCR . Streptococcus pneumoniae and Haemophilus influenzae reacted only with their corresponding probes , and no cross reaction of the bacterial universal probe with virus and fungi was noted .

对猪细小病毒DNA进行斑点杂交，两种探针均为阳性，而对照的猪瘟病毒、猪伪狂犬病毒、乙型脑炎病毒及PK－15细胞的核酸均为阴性。

Dot blot hybridization with the two probes showed the positive result for PPV DNA , but negative for the nucleic acid samples obtained from Hog cholera virus , Pseudorabies virus , Japanese B Encephalitis virus and PK 15 cells .

方法采用核酸分子原位杂交，应用RNA探针对32例TCC组织与8例正常组织的DDX24基因表达情况进行检测。

Methods The expression of DDX24 new gene was detected by using in sity hybridization with RNA primer in32 cases of TCC and8 cases of normal bladder tissue .

目的：为提高原位杂交所用cDNA探针的杂交效率。方法：采用正义引物PCR法及正义、反义引物PCR法制备地高辛（Dig）标记的TGFβcDNA探针。

To improve the hybridizing efficiency of cDNA probe used in situ hybridization the sense primer PCR ( SPCR ) as well as both sense and antisense primers PCR ( SASPCR ) were adopted to generate the Dig labeled TGF_ β cDNA hybridizing probe .

采用网格定位的方法对采集的图像进行了分析，获得杂交后基因芯片探针二维荧光信息的关键特征。

We gain the key character of hybridized gene-chip '2-D fluorescence information by gridding .

以上各温度杂交时，内参探针均有强阳性信号。

When hybridizations were made at above different temperatures , internal reference probes had strong positive signals . 4 .

利用这两种探针分别对雄性毛冠鹿的中期染色体进行原位杂交，涂染探针的杂交得到了阳性结果。

Then in situ hybridization of metaphase karyotype of male tufted deer are made use this two kind probes . The paint probe detected positive result .

随着氨基探针浓度的增加，杂交信号增加，探针达到一定浓度后信号不再增加，形成一个平台，说明连接于玻片表面的探针已达到饱和。

An increase in amino probe concentration brings about an increase in hybridization signal which reaches a threshold corresponding to the saturated concentration of amino probes bounded onto a glass slide surface .

本文介绍了核酸探针和制备技术，标记技术、核酸分子杂交方法及核酸探针的应用状况。

The nucleic acid probe , manufacture and labelling technology , hybrid method of nucleic acid molecule , and the current application status of nucleic acid probe were introduced in this paper .