测序技术

结论：焦磷酸测序技术是一种简便、高效、高通量、高灵敏度、高准确度的HPV分型检测方法。

The pyrosequencing is simple , high efficiency , high flux , high-throughput high sensitivity and high accuracy method of typing HPV .

与传统Sanger法相比，焦磷酸测序技术是一种实时的DNA测序技术，具有快速、高效、成本低的特点。

Pyrosequencing is a real-time DNA sequencing method , which is fast , efficient and low cost , comparing to that based on Sanger reaction .

用焦磷酸测序技术研究猪线粒体细胞色素B基因单倍型

Porcine Mitochondrial Cytochrome B Gene Haplotypes Detection by Pyrosequencing

综述了DNA测序技术。

A review is given on the technology of DNA sequence analysis .

聚合酶链式反应扩增DNA直接测序技术基因诊断β&地中海贫血

Detection of β - thalassemic mutations by direct genomic sequencing of polymerase chain reaction amplified DNA

随着DNA测序技术的完善和普及，分子系统学取得了长足的进展。

As DNA sequencing technology have improved and the molecular systematics has been made considerable progress .

而DNA测序技术的革新和进步，导致了生物信息数据的爆炸式增长。

Meanwhile , the innovation and progress of DNA sequencing technology result in bioinformatics massive data growth .

发展中的DNA测序技术

Developing DNA Sequencing Technique

结论：通过序列差异比较分析，DNA测序技术可成为三七正品基原鉴定的准确、有效手段。

Conclusion : DNA sequencing is an accurate and reliable method in origin identification of the genuine notoginseng .

PCR产物直接测序技术中影响因素的研究

Factors that Influence Direct Sequencing of PCR Products

利用高通量测序技术发现植物小分子RNA研究进展

Progress in Plant Small RNA Research via High-Throughput Sequencing

PCR直接测序技术是PCR扩增与核酸测序技术相结合的一种方法。

PCR direct sequencing is a method which com-bined PCR amplification with nucleic acid sequencing technique .

HLA测序技术在非亲缘性脐血移植应用价值探讨

Clinical Application of HLA Sequencing in Unrelated Umbilical Cord Blood Transplantation

基因测序技术在中药质量研究中的应用（Ⅱ）&山药基原的DNA测序鉴别

Application of gene technology in quality control of Chinese materia medica ⅱ . Identification of Chinese Rhizoma Dioscoreae by DNA sequencing

利用DNA池和测序技术快速筛查SNPs及估算基因频率

Rapidly Screening SNPs and Estimating Allelic Frequencies by DNA Pooling and Sequencing

伴随着DNA测序技术的发展而兴起的分子系统学研究手段近年来越来越多的渗透到苔藓植物学各研究领域。

With the development of DNA sequencing , molecular systematics have infiltrate through more and more research field of bryophyte in recent years .

结果表明，基于PCR及直接测序技术的分子鉴定可以达到准确鉴定蓟马物种之目的。

The results show that molecular identification based on PCR and direct sequencing is desirable for identifying thrips species .

多重PCR和DNA测序技术检测胃癌APC基因15外显子突变

Detection of mutation at exon 15 of APC gene in gastric cancer with multiplex PCR and DNA sequencing

方法：应用PCRSSCP银染和DNA测序技术对31例膀胱癌组织进行检测。

Methods 31 cases of bladder carcinoma were detected by PCR SSCP silver staining and DNA sequencing .

采用常规PCR和测序技术对临床上诊断为强直性肌营养不良的24例患者及其亲属进行基因诊断。

Polymerase chain reaction and sequencing were applied to identify the genetic diagnosis of 24 DM patients and their family members .

DNA测序技术的自动化使得人类基因组测序工作在启动10年后就已接近完成。

Since the automation of DNA sequencing technology , after about a decade the Human Genome Project is going to be fulfilled within the next year .

利用PCR和DNA测序技术扩增了15个中国家鹅品种线粒体DNA控制区部分序列（1042bp）。

The 1 042 bp control region of mitochondrial DNA from 84 geese of 15 domestic goose breeds was sequenced and genetic differentiation was analysed .

方法采用多重PCR、DGGE电泳和DNA测序技术检测76例大肠癌APC基因15外显子突变；

Methods APC mutation was measured with multiplex PCR , denaturing gradient gel electrophoresis and DNA sequencing ;

随着大量被转化植物群体的获得，以及基因组测序技术的发展，TDNA标签逐渐被用于基因功能的确定。

With the development of genome sequencing , coupled with access to large populations of transformed plants . T-DNA tagging is being used increasingly to determine gene function .

现在，新一代测序技术不断发展，但测序过程中DNA分子已被打碎成碱基片段，于是从头测序被提出来了。

Now , a new generation of sequencing technology is developing on and on , but in the process of sequencing , DNA has been broken into fragments .

运用PCRmapping、DNA测序技术和生物信息学手段，对一株临床分离耐亚胺培南铜绿假单胞菌进行整合子结构分析。

With PCR-mapping , DNA sequencing and bioinformatics approach , structure of an integron in a clinical isolate of Pseudomonas aeruginosa resisting to imipenem was studied .

对RET原癌基因第10和第11外显子，采用DNA测序技术进行基因突变筛查。

The PCR product of exon 10 and exon 11 was used to molecular analysis of the RET proto-oncogene .

随着基因组学和DNA测序技术的迅速发展，微生物的全基因组测序已经变得越来越便捷和高效。

As a consequence of the rapid development of genomics and DNA sequencing technology , whole genome sequence of microorganisms now is becoming more and more common and efficient .

本文简要介绍了其中DNA生物传感器、DNA芯片、DNA测序技术、混合DNA样品池、微芯片实验室的结构原理。

The structure principle of DNA biosensor , DNA chip , DNA sequencing technique , pooled DNA , Laboratory on chip ( LOC ) are introduced in this paper .

在产前诊断过程中，我们应用PCR后直接DNA测序技术发现一种以前未描述过的突变：β-30（T→C）。

During the course of prenatal diagnosis we discovered a previously undescribed β - 30 TATA box mutation ( T → C ) by using PCR and direct DNA sequencing .