杂交瘤技术

方法：采用杂交瘤技术制备单克隆抗体（McAb）。

Methods : Monoclonal antibodies ( McAb ) were prepared by hybridoma technique .

目的：通过杂交瘤技术，制备出鼠抗人IgG单克隆抗体，并将其运用到结核抗体IgG检测试纸条中，研制出能快速灵敏的检测出结核抗体IgG的检测试纸条。

Objective : To prepare the monoclonal antibody of mouse anti-human IgG by hybridoma technique , and was applied in the preparing of the colloidal gold strip .

用分离的IgG免疫得兔抗小鼠IgG血清可用为淋巴细胞杂交瘤技术中的试剂。

The Rabbit-anti-mouse IgG could be used as the reagent in the lymphocyte hybridization technique .

利用杂交瘤技术制备了11株抗人绒毛膜促性腺激素（hCG）的单克隆抗体。

Eleven strains of anti-hCG monoclonal antibodies were prepared .

方法：采用二次杂交瘤技术制备抗人喉癌/抗VEGF双功能抗体。

Methods : The second set hybrid tumor technology was applied to prepare an anti-human laryngeal carcinoma and anti-VEGF bifunctional antibody .

方法1.利用合成肽及杂交瘤技术建立抗人VEGF单克隆抗体（McAb）杂交瘤细胞株E11。

Methods 1.To build a hybridoma cell line secreting monoclonal antibody ( McAb ) against VEGF .

应用杂交瘤技术将免疫小鼠的脾细胞与小鼠骨髓瘤细胞（SP2/0）融合，建立分泌抗GST的单克隆抗体杂交瘤细胞株。

The hybridomas were obtained by fusing mouse myeloma cells SP2 / 0 with splenocytes from the mice immunized with GST .

本文采用杂交瘤技术得到能分泌伪狂犬病病毒（PrV，Pseudorabiesvirus）特异抗体的杂交瘤细胞。

Hybridoma cells secreting specific antibodies to Pseudorabies virus ( PrV ) were obtained by using hybridoma techniques as described in the paper .

背景与目的：用杂交瘤技术制备脐血树突状细胞（DC）和食管癌细胞的融合瘤苗，探讨其在体内诱导抗肿瘤效应。

BACKGROUND & AIM : To prepare a fusion vaccine with esophageal carcinoma cell ( EC109 ) and dendritic cells ( DC ), and to study the protective and therapeutic effects against EC109 cells .

方法采用杂交瘤技术，将血吸虫成虫细胞与Sp2／0骨髓瘤细胞融合，HAT选择性培养。

Method Cells from adult Schistosoma japonicum were fused with Sp 2 / 0 mouse myeloma cells , and cultured with HAT media .

以鸡胗为材料提纯结蛋白，经鉴定后免疫BALB/c小鼠，采用杂交瘤技术建立了能稳定分泌抗结蛋白单抗的杂交瘤细胞株AD1，其染色体中位数为77。AD1单抗属小鼠Igg1亚类；

BALB / c mice were immunized with desmin isolated from chicken gizzard .

单克隆抗体技术（monoclonalantibodytechnique）又称杂交瘤技术，是20世纪70至80年代诞生的生物高新技术。

The monoclonal antibody technology ( monoclonal antibody technique ) is also called the hybridoma technology , and it is a high and new biological technology which was borned in the 70s and 80s of 20th century .

利用噬菌体抗体库技术，可克服杂交瘤技术制备人源性mAb的复杂过程，而且可筛选出多种特异性抗体，因此得到广泛应用。

Phage antibody library technology can overcome the complex course to gain human mAb by hybridoma technology and we can screen many specific antibody .

方法：结合O-羧甲基羟胺法和EDC法制备α-ZER-BSA偶联物，免疫BALB/c小鼠，采用杂交瘤技术制备mAb。

METHODS : α - ZER was conjugated to BSA as immunogen to immunize BALB / c mice and mAb were prepared by hybridoma technique .

用杂交瘤技术建立了两株稳定分泌抗波形蛋白单克隆抗体（IgG1亚类）的杂交瘤细胞株：H1C2和I3B2；

( Vim ) isolated from pig lens . Two hybridoma cell liaes ( H1C2 and I3B2 ) continuously secreting anti-Vim McAb ( IgG1 subclass ) were established by the hybridoma technique .

用纯化蛋白作为抗原免疫小鼠，通过杂交瘤技术建立了抗HD-1δ-内毒素蛋白的单克隆抗体杂交瘤细胞株。

Monoclonal antibody against delta-endotoxin protein was ra-ised by the hybridoma technique and detected by an indirect enzyme .

目的应用杂交瘤技术制备抗脊髓灰质炎病毒Ⅱ型Sabin株D抗原的单克隆抗体。

Objective To prepare and identify monoclonal antibody ( McAb ) against the D-antigen of poliovirus type ⅱ Sabin strain .

第4次免疫后，通过淋巴细胞杂交瘤技术建立分泌FB1单克隆抗体的杂交瘤细胞株，融合率为96%，阳性率为98%。

The fusion rate was 96 % , the positive rate was 98 % .

方法：用EBV感染的人B淋巴细胞（RPMI－8866）免疫BALB／C小鼠，采用杂交瘤技术，细胞ELISA和Western－bloting等方法制备、纯化和鉴定了CD23McAb。

Methods : BALB / C mice were immunized by injecting human B lymphocytes infected with EBV cell line RPMI-8866 . And preparing , purifying and evaluating the CD 23 McAb had been done with hybridoma technique , cell ELISA and Western-blotting methods .

应用杂交瘤技术建立了三株分泌牙龈类杆菌单克隆抗体的细胞系CY4、CY5和CY6。

Three hybridoma cell lines CY-4 . CY-5 and CY-6 were established by hybridoma technique and the three strains of hybridoma cells could secreted monoclonal antibody against bacteroides gingivalis .

用淋巴细胞杂交瘤技术研制出11株抗小鹅瘟病毒（GPV）单克隆抗体。

Eleven hybridoma cell lines , secreting neutralizing monoclonal antibodies ( MAbs ) to Goose Parvovirus ( GPV ) were developed by B lymphocyte hybridoma technigue .

采用杂交瘤技术，建立了稳定分泌抗CysC单克隆抗体的2株杂交瘤细胞株，将其中1株细胞注射于小鼠腹腔，得到了富含CysC单克隆抗体的小鼠腹水。

Two hybridoma cell lines were achieved successfully with hybridoma technique , which could stably secret specific McAb against Cys C. After one of the hybridoma cell lines was injected into mice abdominal cavity , the ascites abundant in McAb was obtained .

本实验应用淋巴细胞杂交瘤技术，建立了分泌抗布鲁氏菌弱毒疫苗M5（或M590）菌株的株系特异的单克隆抗体（McAb），杂交瘤细胞株（IC11）。

A hybridoma cell line ( Jc-11 ) . which secretes a strain specific monoclonal antibody ( McAb ) against Brucella Vaccine strain MS ( or M5 - 90 ), was established by lymphocyte hybridoma technique .

利用杂交瘤技术建立分泌抗旋毛虫肌幼虫排泄分泌（ES）抗原单克隆抗体（McAb）杂交瘤细胞株，并对其及分泌的McAb进行鉴定。

This study aimed to establish B cell hybridoma strain secreting monoclonal antibodies ( McAb ) specific to excretory-secretory ( ES ) antigen of muscle larvae of Trichinella spiralis applying to hybridizable technique and the McAbs were characterized .

方法以人脑恶性胶质瘤细胞系SHG-44为抗原免疫Balb/c小鼠，通过杂交瘤技术获得稳定分泌抗胶质瘤McAb的杂交瘤细胞株。

Methods The Balb / c mice were immunized with the malignant human brain glioma cell SHG-44 as antigen and the hybridoma cell line secreting anti-glioma McAb steadily was obtained by using hybridoma technique .

方法：以人多发性骨髓瘤细胞转人B71基因细胞株XG7B7为免疫原，采用B淋巴细胞杂交瘤技术制备鼠抗人B71分子单抗；

Methods : The B lymphocytes hybridization technique was applied by using XG7 B7 cell , a multiple myeloma ( MM ) cell line transfected with human B71 gene , as immunogen ;

方法：以含hCE-Ⅱ的人肝脏微粒体蛋白混合抗原免疫BALB/c小鼠，采用杂交瘤技术制备鼠抗hCE-Ⅱ的mAb，并用Protein-G亲和层析法纯化mAb。

METHODS BALB / c mice were immunized with human liver microsome proteins , and mAb was prepared by hybridoma technique . The crude mAb was purified by protein-G affinity chromatography .

用his-CD26融合蛋白作为抗原足垫快速法免疫BALB/C小鼠，采用杂交瘤技术，制备抗CD26单克隆抗体。

BALB / C mice were immunized with his-CD26 fusion protein and monoclonal antibodies against CD26 were prepared with hybridoma method .

目的利用杂交瘤技术制备抗猪囊虫单克隆抗体，建立抑制性酶联免疫吸附法I-ELISA并应用于临床。

Objective To prepare monoclonal antibody ( McAb ) against cysticorus cellulose antigen by hybridoma technique , and to establish I-ELISA method for clinical application .

[方法]采用杂交瘤技术，获得15株特异性单克隆抗体，随后用酶联免疫吸附试验（ELISA）、免疫印迹法（WESTERNBLOTTING）和间接免疫荧光试验（IFA）对部分免疫显性抗原进行分析。

[ Methods ] 15 monoclonal antibodies ( McAbs ) against T s muscle larva ( ML ) soluble antigens were obtained by using hybridoma technique . The reactivity of monoclonal and polyclonal antibodies were tested by ELISA , Western blotting and indirect immunofluorescence assay ( IFA ) .