末端标记

原位末端标记法和DNA凝胶电泳检测心肌细胞凋亡。

Myocardial apoptosis was detected with in situ end labeling and DNA ladder .

原位DNA末端标记法检测核DNA断裂。

Nuclear DNA fragmentation was observed with in situ end labeling of DNA method .

原位末端标记检测病毒性肝炎肝组织细胞凋亡相关线状断裂DNA

In situ detection of DNA strand breaks associated with apoptosis in viral hepatitis

采用DNA原位缺口末端标记检测角膜移植片中的凋亡细胞。

TUNEL was used to detect the cell ′ s apoptosis in the corneal grafts .

骨髓标本中免疫组织化学及DNA原位末端标记双重染色技术的应用

Application of double coloration technique of immunohistochemistry and DNA in situ end labelling in bone marrow samples

利用针对表位标签的单克隆抗体进行westernblot分析，验证C末端标记目的蛋白的表达。

The expression of the tagged protein was detected with Western blot by using monoclonal antibody to the epitope .

DNA缺口末端标记技术检测病毒性肝炎肝组织细胞凋亡

In Situ Detection of Apoptotic Cells in Liver Tissues from the Patients with Viral Hepatitis with DNA Nick End Labeling Technique

原位末端标记法检测DNA断裂；原位末端标记染色评价细胞凋亡率。

DNA fragmentation was measured by nick end labeling ( TUNEL ) method ; Apoptosis of the MSCs was detected by TUNEL .

采用末端标记引物法进行双链DNA循环测序是实验室快速、准确获得双链DNA模板序列的有效方法。

A rapid , efficient method tO sequence double-stranded DNA template and PCR product , has been developed by using end-labeling cycle sequence .

运用原位末端标记技术检测大鼠缺氧缺血性脑损伤中III型细胞死亡

Discrimination of the type III cell death in hypoxic-ischemic brain damage in rats by in situ end labeling

方法骨髓细胞半固体培养和悬浮培养，DNA凝胶电泳，DNA缺口末端标记（TdT），电镜观察。

Methods Semisolid culture , DNA agarose electrophoresis , in situ terminal deoxynucleotidyl transferase assays , view with electromicroscope .

原位末端标记技术（TUNE）检测见有凋亡细胞散在分布；

The apoptotic cells were detected by in sitU TdT-mediated dUTP nick end labeling ( TUNEL ) .

化学荧光法检测Bcl-2的细胞表达；电镜观察、流式细胞仪与原位末端标记方法检测细胞凋亡情况。

Bcl-2 expression by fluoresce and cell apoptosis by electronic microscopy , flow cytometry and in situ terminal staining .

以DNA-梯形凝胶电泳、凋亡细胞原位末端标记法（TUNEL）对急性脑损伤后鼠脑皮层、海马区神经元DNA损伤情况进行观察。

The neuronal DNA injury in the brain cortex and hippocampus was detected by DNA Ladder gel electrophoresis and TUNEL stain .

方法：琼脂糖凝胶电泳、DNA裂解率的测定和原位末端标记法（TUNEL）。

Methods : Agarose gel electrophoresis , TdT mediated dUTP nick end labeling ( TUNEL ) and fluorescence spectrophotometry were used .

方法用原位末端标记法以及镀铜镉还原法对实验性OA软骨细胞凋亡及关节滑液中的NO含量进行检测。

Methods Apoptosis of chondrocytes was determined by TUNEL and the levels of NO in SF by reduction .

以缺口末端标记法（Tunel法）标记凋亡细胞。

The apoptotic cell was labeled by TUNEL .

用原位末端标记技术和免疫组化方法检测血肿周围神经凋亡和核因子κB表达的动态变化。

The dynamic change of apoptosis and the expression of NF - κ B in the tissue surrounding hematoma were examined with in situ end-labeling ( ISEL ) and immunohistochemical method .

方法用细胞形态学观察，DNA琼脂糖凝胶电泳，原位末端标记法检测20例K562细胞、23例CD3AK细胞诱导的K562细胞凋亡率。

Methods Apoptosis was detected by TDT end labelling technique , morphologic observation and DNA agarose gel electrophoresis .

末端标记法（TUNEL）行细胞凋亡检测；

Methods The apoptosis in cardiomyocyte was detected by end labeling ( TUNEL ) assay .

采用原位缺口末端标记方法（TUNEL）检测大鼠肺动脉平滑肌细胞的凋亡，并行图像分析计算单位面积细胞凋亡率；

VSMC apoptosis was measured by terminal deoxynucleotidyl transferase biotin nick end labeling ( TUNEL ) .

原位末端标记法（TUNEL法）检测胃粘膜细胞凋亡指数；

The in situ TUNEL method was used in the apoptosis index ( AI ) detection .

用免疫组织化学法检测瘤组织中PCNA及Bcl-2蛋白的表达。采用原位末端标记法检测瘤组织中的凋亡细胞。

Expression of PCNA and Bcl-2 in carcinoma tissues was detected by immunohistochemistry , and apoptosis of cells were labeled with TUNEL .

方法：dUTP缺口末端标记法和AnnexinV法；

METHODS : Terminal dUTP nick end labeling ( TUNEL ) and Annexin V assay were used .

12h和1d的组织用TdT介导duTP缺口末端标记法（TUNEL）检查细胞凋亡。

Cells apoptosis was tested by TdT mediated dUTP nick end labelling ( TUNEL ) .

用DNA缺口原位末端标记（TUNEL）技术检测细胞凋亡；

Apoptosis was detected using DNA in situ terminal deoxynucleotidyl transferas ( TDT ) mediated deoxyuridine triphosphate ( dUTP ) biotin nick ending labeling ( TUNEL );

应用TUNEL原位末端标记法定量分析细胞凋亡以及NADPHd组织化学染色法观察细胞NOS的表达。

The neuronal apoptosis were measured by TUNEL staining . The NOS activity was determined by NADPH-d histochemical analysis .

利用电镜和末端标记法（TUNEL）观察胎儿卵泡形成过程中卵细胞凋亡的发生。

Electron microscope and TUNEL method were used to examine the occurrence of oocytes apoptosis during human fetal folliculogenesis .

原位末端标记细胞凋亡显示模型组细胞凋亡阳性数明显高于正常组（P0.05），药物干预组明显低于模型组（P0.05）。

Apoptosis TUNEL showed positive cells in model group were obviously higher than the normal group , drug intervention group was lower than the model group . 4 .

用免疫组化及DNA缺口末端标记法观察了正常及骨关节炎软骨组织中c－myc，bcl－2的表达以及软骨细胞的凋亡情况。

The authors have investigated the expressions of c-myc , fas , bcl-2 and apoptosis of chondrocytes in normal and osteoarthritic articular cartilage using immunohistochemistry and TUNEL .