克隆片段

间日疟原虫特异性DNA克隆片段的序列测定和分析

Sequencing and analysis of cloned DNA fragment specific for Plasmodium vivax

DNA序列分析中克隆片段在M13的缺失及质粒载体的应用

Deletion of Cloned DNA Fragment in M13 and Application of Bluescript Vector for Subcloning and Sequencing

并通过Southern杂交证明这2个克隆片段分属于大麦基因组特异的多拷贝重复序列和小麦与大麦基因组共有的寡拷贝序列。

Southern hybridization indicated that the two cloned DNA fragments belong to barley genomic specific high-copy repeat sequence and low-copy sequence in wheat and barley genomes respectively .

对虾白斑症病毒（WSSV）部分克隆片段的序列测定及PCR扩增

Sequencing of Partial Genome DNA of Penaeid Shrimp White Spot Syndrome Virus ( WSSV ) and Its Amplification by PCR

结果：扩增出长1601bp的cDNA序列，测序证实：克隆片段与Genebank该基因序列同源性为100%；

Results : The 1 601 bp length cDNA was obtained , and result of sequencing confirmed the cloned cDNA sequence is completely coincidence with that of glucocorticoid receptor in Genebank .

利用水稻双子房突变体为材料，设计简并引物，扩增类copia逆转座子中最保守的RT区域，得到了3个克隆片段。

Three clones that related to the conservative reverse transcriptase ( RT ) domain of copia-like retrotransposons were amplified from the total DNA of a twin-ovary mutant .

将4个含有不同大小启动子区的克隆片段与GUS基因编码区连接构建成嵌合基因，通过基因枪轰击转入香蕉叶、根和果实的细胞后。

The 5 ′ - flanking fragments in different length were fused to the coding sequence of GUS gene . These constructs were delivered to leaf , root and fruit cells of banana via particle bombardment .

取对虾（PenaeidShrimp）白斑综合征病毒（WSSV）基因组DNA随机文库中1个约8kb的克隆片段进行测序。

A genome DNA fragment about 8 kb from random library of white spot syndrome virus ( WSSV ) of shrimp ( Penaeid shrimp ) was sequenced and the DNA sequence was analyzed by DNA star software .

4个抗性克隆片段均为跨膜结构，属于LZ-NBS-LRR类抗病基因相关片段。

Four clones possess the construction of transmembrance segment , and they are all the members of resistance genes characterized with LZ-NBS-LRR .

结果表明：克隆片段全长为935bp。

The results showed that the length of the clone sequence was 935 bp .

对这一克隆片段进行了序列分析。

They were cloned and sequenced .

对克隆片段进行了酶切位点分析，并做了限制性内切酶图谱。

The sites of restriction endonucleases were analysed and restriction site map of the cloned fragment was drawn .

家蚕丝素重链启动子克隆片段在家蚕体内和昆虫培养细胞内的渗漏表达

Leaked Expression of Cloned Fibroin Heavy Chain Promoter Sequence in Silkworm , Bombyx mori , and Insect Cultured Cells

牛CSN2全基因相应区段同源性分别为99.0%和98.0%，证明克隆片段为奶牛β-酪

The relative region of bovine β - casein gene was 99 % and 98 % respectively , which proved

对重组质粒进行限制性内切酶分析和基因测序，证实了克隆片段的可靠性。

The recombinant plasmid of pGTE-TK was identified by restriction enzyme analysis and sequencing , which proved completely its validity .

在这些克隆片段中80%的插入片段是重复顺序，少部分是单拷贝序列。

Southern blot analysis showed that most of these cloned fragments were in repetitive sequence and a few in single copy sequence .

PCR扩增阳性克隆插入片段。

The inserts were amplified by PCR .

该方法对于从变性和非变性大page胶上回收克隆目的片段具有普遍意义。

This method is normally suitable to recovery and cloning of positive bands from denaturing and nondenaturing page gel .

据此推断克隆的片段为PPO基因片段。

So , it was thought to be PPO gene fragment .

快速克隆DNA大片段的方法

Fast Method for Cloning DNA Big Fragments

结果经酶切和基因测序证实，克隆的基因片段为带有信号肽序列的VIP基因；

Results : The cloned VIP cDNA was confirmed by enzyme digestion and DNA sequencing .

而且，由于在哺乳动物基因组文库构建中，克隆的大片段DNA在大肠杆菌中十分稳定，所以基于F质粒的克隆技术已经被广泛接受。

Furthermore , F-plasmid cloning technology has gained widespread acceptance for the construction of mammalian genomic libraries due to their stable maintenance of large foreign DNA inserts in E. coli .

方法：以弓形虫RH株速殖子感染大鼠血清作为探针筛选弓形虫cDNA文库，对阳性克隆的插入片段进行PCR扩增及DNA序列测定。

Methods Rats were infected with Toxoplasma gondii RH strain and their serum was used as a probe to screen Toxoplasma gondii tachyzoite cDNA expression libraries . The positive clones were analyzed by PCR amplification and DNA sequencing .

对克隆的扩增片段测序及同源分析结果显示：个别扩增条带尽管大小一致，实际上所包含基因却还存在差异，也在一定程度上说明RAPD方法存在一定的局限性。

The sequences of PCR products and evolutionary relationship analysis showed that some PCR products included different genes though they were the same in size , which revealed that RAPD is a local method to a certain extent to differentiate bacteria species .

结果表明，这种方法能同时克隆RDV基因片段S11、S12，是一种有效实用的dsRNA病毒基因组克隆方法。

The results showed : S11 and S12 segments of RDV could be simultaneously cloned . The method for cloning viral dsRNA genomes is practicable and valid .

对随机挑选的452个BAC克隆的插入片段进行鉴定估计文库的平均插入为118kb，文库的基因组覆盖率为13.34倍，预计从文库中筛选到单拷贝基因的机率为99.984%。

Average insert size of the library is 118 kb evaluated from analysis of 452 BACs randomly picked from library . So the genome coverage of the library is 13.34 and the possibility to find a single-copy gene in the library is 99.984 % .

第三组观看和第一组同样的《逃出克隆岛》片段，但是没有声音。

The third group watched the same excerpt from " The Island " as the first group , but without sound .

其中661克隆的插入片段为477个碱基序列，其一端120bp与探针序列一端完全重叠。

The insert of clone 661 , has a 478 bp sequence and overlayed with the sequence of DNA probe on one side .

结果文库中442个克隆含有插入片段，斑点杂交获得的阳性克隆测序，获得39条差异基因片段和2条全长序列基因。其中高表达16条，含未知功能基因片段3条；

Results The subtracted library contained 442 clones with inserts , after being sequenced and analyzed , 16 clones were up-regulated , including 3 new genes ;

目的探讨大鼠角膜碱烧伤后差异基因表达情况，克隆目的基因片段并了解其同源性，检测有无新基因产生，从分子角度阐明角膜碱烧伤后变性蛋白产生的分子生物学基础。

Objective To study the condition of differential gene caused after corneal alkali burns in rats and clarify the molecular biological foundation of corneal denatured protein .