晶体蛋白

晶体蛋白βB2对眼晶体老化的影响

Effect of crystallin β B_2 on the aging of lens

α-晶体蛋白干预大鼠RGCs过氧化损伤作用实验研究给药48h后，分别取培养上清及贴壁细胞从以下四方面进行指标测定：1.观察培养细胞的过氧化损伤；

Experimental study of intervention effect of α - crystallin on superoxide damage RGCs exposed to H_2O_2 24 hours , the medium and the cell were assayed respectively as follow : 1.Observing the superoxide damage in different groups ;

目的鉴定γB－晶体蛋白非酶糖基化位点。

Objective To identify the glycation sites of lens γ B-crystallin .

苏云金杆菌4.0718质粒上杀虫晶体蛋白基因的PCR分析

PCR-based Analysis of Insecticidal Crystal Proteins Genes on Plasmids from Bacillus Thuringiensis 4.0718

利用PCR技术直接鉴定苏云金芽孢杆菌杀虫晶体蛋白基因型的快速方法

A rapid method in direct identification of Bacillus thuringiensis ICP gene type by PCR

方法用离子交换HPLC纯化小牛晶状体γB－晶体蛋白，与果糖保温后用糜蛋白酶水解。

Methods γ B-crystallin was purified by ion-exchange HPLC , incubated with fructose and digested with chymotrypsin .

α-晶体蛋白与对照蛋白比较，具有特异性保护糖基化诱导SOD和CAT的失活。

α crystallin specifically protected antioxidant enzymes against glycation induced inactivation compared with control proteins .

昆虫中肠Bt杀虫晶体蛋白毒素受体氨肽酶N的研究进展

Progress of studies on insect midgut aminopeptidase N serving as Bacillus thuringiensis insecticidal crystal protein toxin receptor

本文对Bt杀虫晶体蛋白分类方法、蛋白活性以及蛋白之间的协同作用进行综述。

Classification , toxicity and synergistic effect of Bt crystal proteins are reviewed .

昆虫中肠Bt晶体蛋白受体的研究进展

Progress in studies on insect midgut membrane receptor for Bacillus thuringiensis δ endotoxin

Bt杀虫晶体蛋白的土壤残留及其对土壤磷酸酶活性的影响

Persistence of Bt toxin in soil and its effects on soil phosphatase activity

十株Bt野生菌杀虫晶体蛋白及基因型分析

Analysis of insecticidal crystal protein and its cry-type genes of ten Bacillus thuringiensis isolates

Bt黑色素对杀虫晶体蛋白的紫外保护

UV-Protection of Bt Melanin to Insecticidal Protein

结果：正常和硒性白内障幼鼠晶状体α-晶体蛋白与对照蛋白比较，具有特异性抑制CAT热凝聚的作用；

Results : α - Crystallin in both normal and cataractous lenses specifically prevented cata-lase against thermal aggregation compared with control proteins .

50例老年性白内障晶体蛋白含量及SDS聚丙烯酰胺凝胶电泳分析

The determination of protein and analysis of sDs polyacrylamide electrophoresis of cataract Iens in fifty eases

βB2晶体蛋白基因敲除小鼠模型的建立

Establishment of a β B2 crystallin gene knockout mice model

晶体蛋白βA1基因缺失突变导致常染色体显性遗传核性先天性白内障

Autosomal dominant congenital nuclear cataract caused by a deletion mutation in the β A1-crystallin gene

以苏云金杆菌杀虫晶体蛋白基因为探针，进行Southernblot分子杂交，得到阳性结果。

DNA Southern blot hybridization and polymerase chain reaction showed that some of the plants were positive when probed with the insecticidal crystal protein gene .

结论：成功构建了βB2晶体蛋白基因敲除的小鼠模型。

Conclusion : The β B2 crystallin gene knockout mice model has been successfully established .

以Northern印渍杂交对培养细胞晶体蛋白的特异性表达进行了鉴定，发现原代及第二代细胞内仅存在α晶体蛋白mRNA。

The special expression of crystallins from the cultured cells was identified by Northern blot ,α crystallin mRNA was only found in the primary and the secondary generation cells .

外源性氧化或炎性刺激如H2O2和脂多糖能影响角膜细胞中晶状体晶体蛋白的表达。

Lens crystallin gene expression in cultured corneal cells responded to exogenous oxidative or inflammatory stimuli like H2O2 or lipopolysaccharide .

进一步利用PCR-RFLP方法对菌株XCF4c-1进行杀虫晶体蛋白基因检测正在进行中。

Further study of i-dentification insecticidal crystal proteins genes is ongoing based on PCR-RFLP method .

结果：PCR成功检测出3种基因型；纯合子基因敲除的小鼠未检测到βB2晶体蛋白的表达。

Results : Three genotypes were successfully ( identified ) and expression of β B2 crystallin protein was not detected in gene knockout mice .

而α晶体蛋白不仅能促进RGCs存活，而且对RGCs轴突再生也具有明显促进作用。

Alpha-crystallin can not only promote RGCs survival , but also stimulate axon regeneration .

主要的杀虫成份是杀虫晶体蛋白，由cry和cyt基因编码。

The primarily insecticidal protein was Insecticidal Crystal proteins ( ICPs ) which were encoded by cry or cyt genes .

然而，Bt蛋白酶是否对杀虫晶体蛋白的功效有影响仍然是个争议性的话题。

However , whether the protease profile of a Bt is related to the efficacy of the ICPs is still a subject of controversy .

苏云金杆菌Cyt类杀虫晶体蛋白及其特征

The Characteristics of Bacillus thuringiensis Cyt Insecticidal Crystal Proteins

Bt晶体蛋白Cry1Ac放射免疫检测技术研究

Study on radioimmunoassay of BT cry 1 AC protein

转Bt基因植物中杀虫蛋白的酶联免疫检测技术研究Ⅱ.Bt杀虫晶体蛋白抗体的制备

Studies on the Enzyme-linked Immunosorbent Assay for Detection of Bacillus thuringiensis Insecticidal Protein Expressed in Transgenic Plants ⅱ . Preparation of Polyclonal Antibody to Bt Insecticidal Protein

利用基因工程技术把具有杀鞘翅目活性的Bt杀虫晶体蛋白基因转入植物，培育抗鞘翅目新品种是控制其危害的一条新途径。

The application of Coleoptera - specific Bt toxin proteins by means of the technique of genetic engineering in transgenic plants provides a new way of controlling this pests .