抽提法

方法以上述3种药物治疗实验大鼠肺孢子虫肺炎，治疗结束后收集鼠肺，以酚-氯仿抽提法抽提肺总DNA。

After the therapy , the control and treated rat lungs were collected . Total lung DNA was extracted by phenol-chloroform extraction method .

方法：采用常规酚抽提法从外周静脉血提取DNA，应用多聚酶链反应-单链构象多态性分析技术对被试者的早老素1基因第5号和第8号外显子进行突变检测。

METHODS : The conventional phenol extraction method was used to withdraw DNA from peripheral blood , and polymerase chain reaction-single strand conformation polymorphism analysis ( PCR-SCCP ) was applied to detect the mutation of the 5th and 8th exons in presenilin-1 gene .

通过改良酚氯仿抽提法提取粪便DNA。

DNA was extracted from stools by phenol chloroform extracting method .

按酚/氯仿抽提法提取基因组DNA；

We extracted human genomic DNA using phenol / chloroform extracting ;

血清乙肝病毒DNA煮沸抽提法的建立和应用

Establishment and application of boiling method to extract HBV DNA from serum sample

用改进的酚/氯仿抽提法提取血细胞DNA。

A reformed phenol / chloroform method was used to extract DNA in blood .

用常规酚／氯仿抽提法提取DNA，其浓度用紫外分光光度计测定。

Using conventional phenol / chloroform extraction method extracted DNA , the concentration by UV spectrophotometer .

结果表明，常规酚&氯仿抽提法适合从马血中大量提取DNA。

The result shows that routine phenol - chloroform method was adapt to extract a mass of DNA .

胸/腹水上清基因组DNA的抽提采用常规蛋白酶K消化、酚/氯仿抽提法。

Genome DNA in the fluid supernatant was conventionally extracted by proteinase K digestion followed by phenol / chloroform extraction .

方法分别用传统的酚、氯抽提法和改良法两种不同的方法，提取人全血基因组DNA。

Method An improved agarose gel electrophresis method and the traditional phenol-chloroform one were used to extract human genomic DNA from whole blood .

双剂抽提法提高FCC柴油氧化安定性

Improving Stability of LCO by Double - Solvent Extraction

综合比较，苯酚/氯仿抽提法提取乙醇保存的尾鳍组织基因组DNA是一种行之有效的方法，可以在鱼类分子研究中推广应用。

The phenol extraction of genomic DNA from alcohol-preserved caudal fin is an effective method of promoting and using in molecular studies on fish .

方法：采用酚-氯仿抽提法抽提患者血清DNA，聚合酶链反应扩增血清HBVx基因，琼脂糖电泳观察结果，并对PCR产物行序列分析。

Methods : DNA was extracted from the serum by phenol-chloroform extracted method and HBV X gene was amplified by polymerase chain reaction .

结果：酚抽提法Hp检出率显著低于直接裂解法。

Results The rate of Hp positive by direct cleavage method was higher than by Phenol - chloroform extraction .

方法应用高盐抽提法抽提细胞核基质蛋白，SDSPAGE技术分析白血病细胞与正常骨髓细胞核基质蛋白的变化。

Method Nuclear matrix proteins were extracted under high-salt extraction procedures , and changes of nuclear matrix proteins were analysed by SDS-PAGE .

研究东北虎毛发DNA的水煮抽提法，并将传统的酚仿抽提法和水煮法进行比较。

The DNA extraction from the hairs of the Panthera Tigris Altaica , cooking extraction method in water was studied , and the comparison with the traditional phenol and chloroform method was done .

对传统的Southern杂交法进行了改进：用碱变性和酚抽提法提取重组质粒，省去氯化铯梯度离心；

The procedure of Southern hybridization has been improved as follows : the plasmid DNA which contains DNA fragments for probe was isolated by alkaline lysis without utilizing cesium chloride gradient centrifugation .

改进了用有机溶剂抽提检测一氧化氮（NitricOXide，NO）自由基的方法，并利用有机溶剂抽提法检测了小鼠心肌中NO的含量。

The extraction method with organic solvent extraction to detect nitric oxide was improved , and the production of nitric oxide in mice myocardium in vitro was detected with this method .

二免后一周收蛋并用氯仿抽提法提取卵黄抗体，用间接ELISA法检测抗体效价。

One week after the second immunization , the eggs were collected and the yolk antibodies were extracted , then the titers of the yolk antibodies were detected by indirect ELISA .

对拟步甲科8种昆虫的新鲜标本及无水乙醇浸泡标本，分别用SDS蛋白酶K消化法和乙酸钾抽提法进行基因组总DNA的提取。

This paper reports the extraction genome DNA from eight species of Tenebrionidae insects including the fresh specimens and the preserved specimens in 100 percent alcohol with methods of SDS-proteinase K and KAC .

以上标本每一例分别捣碎，分别用酚-氯仿抽提法提取DNA，紫外分光光度仪测定DNA纯度及含量。

Each tissue sample was ground thoroughly in mortar with pestle . DNA was extracted by the phenol-chloroform method . To determine the concentration and purity of the DNA solution , the absorbance of UV light is measured in a spectrophotometer .

结论头皮针抽提法对改善脑血栓形成后恢复期偏瘫患者运动功能，提高患者ADL能力的作用优于头皮针捻转法；

Conclusion SADM was superior to scalp-acupuncture twisting method in improving the patients ' motor function of extremity and ADL in hemiplegia patients following cerebral thrombosis at convalescent period ;

用热抽提法测定铜中氧时，不但产生CO也生成部份CO2，而大多数商品分析仪器都不能同时检测CO和CO2，使氧分析结果产生误差。

The determination of oxygen in copper by hot-extraction method gives off its oxygen content mainly as CO and partially as CO_2 But most of the commercial analytical instruments for oxygen determination are designed to detect CO only . The evolution of CO_2 leads to erroneous results .

采用溶剂抽提法、元素分析法、VPO平均分子量测定法、H&NMR和IR光谱法测定了几种ETP和中间相沥青的结构参数。

Solvent extraction , elemental analysis , molecular weight determination by VPO , ~ ′ H & NMP and TIR spectroscopy were accomplished for the estimation of structural parameters of ETP precursors and mesophase pitches .

对三种不同的DNA抽提法作了比较，认为用Tween20和NP40作为裂解液与SDS法比较有显著性差异（P＜0.05），且Tween20比NP40更适合。

The results showed that the extraction of DNA with Tween 20 or NP40 had significant difference as compared with SDS ( P < 0.05 ) . The extraction of DNA with Tween 20 was better than with NP40 .

在研究犬STR基因座多态性中，比较使用了饱和苯酚-氯仿抽提法和Chelex-100树脂法，两种方法提取的DNA在扩增后的检测结果均能准确地反映实验结果。

DNA extraction methods by using Chelex - 100 and saturated phenol - chloroform were used respectively in the study of canine STR loci polymorphism . The detecting result of two DNA extraction methods after amplification could reflect the experiment result .

将白细胞匀浆沉淀残渣按Scherrer和Darnell的十二烷基磺酸钠加苯酚的热抽提法抽提。

The iRNA was extracted from the residue of the leucocyte homogenate by a modified method of Scherrer and Darnell using hot phenol with sodium dodecyl sulfate .

制备一系列不同固化温度和固化时间的涂膜，测定涂膜的耐水性及吸水率，用索氏抽提法测定涂膜的固化交联率，确定了固化条件为固化温度160℃时间40min。

The curing regularity of film coating is investigated too . A series of coating films of different curing temperature and curing time were prepared .

采用一种经修改的硫酸铵沉淀及反向抽提法，从猪心肌粗提液中纯化得比活性为60u/mg的乳酸脱氢酶（LDH），纯化倍数为50倍，酶活性回收为78%。

A modified method of ammonium sulfate precipitation and back-extraction was used , lactate dehydrogenase ( LDH ) with the specific activity ( 60.09u / mg ) has been isolated from the crude extract of porcine cardiac muscle . This procedure yields a 50-fold purification with 78 % recovery .

方法用柠檬酸抽提法，从家兔中性粒细胞溶酶体中提出防御素粗制品，经酸性尿素聚丙烯酰胺凝胶电泳（AU-PAGE）分离纯化。

Methods Defensins were extracted from rabbit peritoneal neutrophil granules with citric acid and purified by acid urea polyacrylamide gel electrophoresis ( AU-PAGE ) .