报告基因

转基因植物中报告基因gus的表达及其安全性评价

Biosafety and expression of reporter gene gus in transgenic plants

目的：研究HBV启动子调控下的GFP报告基因在肿瘤细胞中的表达。

Objective : To investigate the expression of GFP reporter gene under the direction of HBV promoters in tumor cells .

应用荧光素酶报告基因技术检测DHMEQ对NF-κB基因活性的影响；

Transcriptional activity of NF - κ B was determined using luciferase reporter gene assay .

人Era不同结构域在酵母双杂交系统中的表达及对报告基因激活作用的检测

Reporter Genes Assay of the Different Domain of Human Era in Yeast Two-hybrid System

双荧光蛋白报告基因分析系统在微小RNA研究中的应用

The application of dual fluorescent protein reporter assay system in microRNA research

人CAT启动子红色荧光蛋白报告基因载体的构建及功能鉴定

Construction and functional identification of red fluorescent protein reporter plasmid for human catalase gene

PET报告基因显像

Investigation progress of PET reporter gene imaging

用免疫狭缝印迹和ELISA方法分析报告基因CAT和目的基因bax在细胞中的表达。

The expression of bax and CAT genes was detected by immuno-slot blot and ELISA .

以GFP为报告基因的酿酒酵母mRNA在哺乳动物细胞中的表达

The expression of green fluorescent protein gene mRNA from yeast in mammalian cell

SA脂质体介导报告基因转染耳蜗的实验研究

Experimental study of transfection of cochlea by SA liposome mediated reporter gene

利用荧光素酶报告基因检测技术确证ERδ5对ERα转录活性的影响。

The effect of ER δ 5 on ER transcription was investigated by luciferase reporter assay .

细胞内活化的Notch途径报告基因质粒的构建和鉴定

Construction and identification of a reporter plasmid for analyses of Notch signaling pathway

报告基因标记的HCV复制/感染模型的建立

Establishment of Reporter HCV Replication / Infectious Models

用双荧光素酶报告基因检测IL-8激活程度。

IL-8 gene promoter activation was detected using dual luciferase reporter gene assay .

以gfp为报告基因研究蛋白亚细胞定位

Experience of gfp as A Report Gene to Study Subcellular Localization

家蚕精子介导报告基因gfp和egfp转移技术的研究

Sperm-mediated Repoter Gene gfp and egfp Transfer in the Silkworm

以gfp作为报告基因研究了烟草中蛋白亚细胞定位。

GFP is widely used as a report gene to study protein subcellular localization .

结论本试验建立并采用的基于报告基因的体外雌激素检测方法是可行的，NP和BPA有一定的雌激素样活性作用。

Conclusion The assay we established is usful , NP and BPA showed estrogenic activities .

其启动子可以驱动报告基因GFP在斑马鱼胚胎骨骼肌以及心肌中特异性表达。

Its promoter can drive reporter gene-GFP in skeletal and cardiac muscles in zebrafish embryos .

GFP为外源报告基因在地衣芽孢杆菌20386中表达的研究

Foreign Gene Expression in Bacillus Licheniformis 20386

将含有报告基因LACZ的重组腺病毒载体从大鼠鼻腔转移至脑的实验研究

An adenoviral vector with a report gene Lac Z transferred to brain from nasal cavity of adult rats

目的：构建含报告基因GFP的人生长激素（hGH）的真核表达质粒。

Objective : To construct the eukaryotic expression vector pcGH - GFP .

放射诱导调控腺病毒介导gfp报告基因在肿瘤细胞内的表达

The Expression of Adenoviral Mediated gfp Reporter Gene in Tumor Cells Induced and Regulated by Irradiation

利用绿色荧光蛋白报告基因定量测定escherichiacoli中UGA密码子的通读效率

Quantitative Analysis of the Read-through Efficiency of UGA in Escherichia Coli with Green Fluorescent Protein

结果和结论1、成功构建了eNOS启动子报告基因载体及ERβ真核表达载体。

The eNOS promoter reporter gene vector and ER β eukaryotic expression vector were successfully constructed . 2 .

含报告基因GFP的hGH真核表达载体的构建

Construction of eukaryotic expression vector of human growth hormone

人Puma启动子荧光素酶报告基因的构建和鉴定

Construction and identification of human Puma promotor luciferase report gene vector

小鼠α4b干扰素报告基因的构建及其真核表达的探索

Constructing and Exploring Eukaryotic Expression of Murine Interferon α 4b Reporter Gene

用Luciferase法检测报告基因表达。

Reporter gene expression was detect by Luciferase . 3 .

以GFP为报告基因优化CNE-2Z细胞脂质体转染条件的实验研究

GFP used as a reporter gene for optimizing lipofection