复制子
- replicon;replicor;duplicon
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这样合成的每一片段DNA称为复制子。
Each segment of DNA thus synthesized is called a replicon .
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HCV的新型体外细胞模型:复制子系统
Novel in vitro cell culture models for HCV : Replicon systems
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来自邻近的复制子的新合成的DNA链又连结在一起。
Newly-synthesized DNA strands from adjacent replicons are ligated together .
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基于甲病毒复制子载体的猪瘟DNA疫苗的免疫效力评价
Alphavirus Replicon - vectored Plasmid DNA - based Vaccine Elicits Protective Immunity Against Classical Swine Fever Virus
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含RNA复制子表达载体介导的生长抑素基因在小鼠肌肉中的表达
Expression of SS gene in the muscle in mice by improved RNA-based replicon vector
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RNA复制子疫苗研究进展
Research Development of RNA Replicon - based Vaccines
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乙肝病毒RNA复制子疫苗、DNA疫苗的构建及其对小鼠免疫效果的比较
Construction of RNA Replicon Vaccine and DNA Vaccine of HBV and Comparison of Their Immune Efficiency in Mice
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RNA复制子疫苗
RNA Replicon - based Vaccines
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这些研究结果为选择性HCV全序列基因组复制子的研究奠定了基础。
These discoveries make it possible for the development of selectable full length genome replicon .
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这些结果说明,DNA形式的西尼罗病毒复制子能够表达外源基因,具有良好的复制活性,为下一步观察该复制子的免疫原性奠定了基础。
All results demonstrated that the DNA-based WNV replicons could express exogenous genes and replicate itself well . This may set a foundation for following observation of replicon immunogenicity . 2 .
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日本脑炎病毒(JEV)复制子表达载体的构建及其鉴定
Construction and Application of Subgenomic Replicon Vectors of Japanese Encephalitis Virus
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基于RNA复制子GHRH基因表达载体的构建及在293细胞中的表达
The Construction of GHRH Gene Expression Vector Based on RNA Replicon and Its Expression in 293 Cell
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基于DNA的Semliki森林病毒复制子载体体内外高水平表达外源基因
High-level Expression of Foreign Genes In vivo and In vitro by Improved DNA-Based Replicon Vector Derived From Semliki Forest Virus
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基于SemlikiForest病毒RNA复制子的表达载体及其衍生的2型猪圆环病毒RNA疫苗的初步研究
Development of a Eukaryotic Expression Vector Derived from Semliki Forest Virus RNA Replicon and a Derivative RNA Vaccine Candidate Against Type 2 Porcine Circovirus Infections
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RNA复制子疫苗克服了传统疫苗和普通DNA疫苗存在的缺点,具有抗原表达效率高、安全性好、应用范围广等优点,因而被视为一种发展前景很好的疫苗形式。
RNA replicon-based vaccine is regarded as a kind of promising vaccine because of its higher expression efficiency of antigen , higher security , wider range of application than classical vaccines and ordinary DNA vaccines .
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目的本研究对原卟啉钠的制备方法进行了改进,以期简化其生产流程。并且体外观察原卟啉钠的细胞毒性作用及其对HCV亚基因复制子细胞模型RNA的抑制效果。
[ Objective ] To explore the improved preparation of protoporphyrin disodium , observe the cytotoxicity of Napp in vitro and its inhibitory effect on HCV subgenomic replicon cell model .
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体外实验Napp对靶细胞安全作用范围较宽,且可有效抑制HCV复制子RNA。
Napp has little cytotoxic effect on target cells and it can effectively inhibit the RNA level of HCV subgenomic replicon cell model .
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利用此蛋白上述两种功能特点,研究者构建了EBV复制子载体,这种载体已被广泛地应用于基因治疗。
On the basis of two functions of EBNA-1 , investigators have constructed successfully EBV-based plasmid , which can be used to gene therapy extensively .
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传统DNA疫苗载体与Semliki森林病毒复制子对HIV-1Pr55~(gag)表达与体液免疫原性的比较性研究
Comparative Study of Expression and Humoral Immunogenicity of HIV-1 Group Specific Antigen Pr55 ~ ( gag ) by Conventional Plasmid Vectors Versus Semliki Forest Virus-Derived Vectors
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XJ-160病毒复制子型表达载体的构建
Construction of Replicon Expression Vector Derived from XJ - 160 Virus
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目的研究塞姆利基森林病毒(Semlikiforestvirus,SFV)衣壳蛋白5′翻译增强区对基于该病毒复制子的HIVGagDNA疫苗抗原表达水平和免疫原性的影响。
Objective To investigate the effects of 5 ′ translation enhancing region of Semliki forest virus ( SFV ) capsid protein gene on the gene expression and immunogenicity of HIV Gag DNA vaccine based on SFV replicon DNA vector .
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如果在这种复制子中插入外源基因,由于复制子RNA在细胞胞浆内大量扩增,就使得外源基因得到有效表达,因此,复制子RNA可作为表达外源基因的良好载体。
When the heterologous genes are inserted into the replicon , amplification of replicon RNA in the cytoplasm of cells makes the heterologous genes express effectively , as a consequence , the replicon RNA can be made the excellent vector for expression of heterologous genes .
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结果表明我们构建的XJ-160病毒复制子型表达载体具有自主复制功能,可以表达异源基因。
The results indicated that the replicon vector derived from XJ-160 virus was self-replicating and that the following gene expression was efficient .
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目的利用塞姆利基森林病毒(SFV)复制子构建新型真核表达载体,并对含HIV-1中国流行株B亚型核心蛋白p24及多表位MEG嵌合基因的核酸疫苗进行表达与鉴定。
Objective To construct a novel eukaryotic expression vector based on Semliki forest virus ( SFV ) replicon and express the DNA vaccine containing subtype core protein p24 and multi-epitope MEG chimeric gene of epidemic strain B of HIV-1 in China .
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结论SFVC蛋白翻译增强区能显著提高SFV复制子DNA疫苗的抗原表达和抗原特异性细胞免疫反应。
Conclusion Antigen expression and cellular immune responses induced by the HIV Gag DNA vaccine based on a SFV replicon DNA vector were significantly improved by fusing the translation enhancing region of SFV capsid gene to the 5 ′ terminus of gag gene .
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丙型肝炎病毒非结构基因复制子模型的建立和应用
Subgenomic replicon model of hepatitis C virus and it 's application
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基于日本脑炎病毒复制子载体系统的疫苗研究
Development of Vaccine Research Derived from JEV Replicon Expression Vector System
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复制子系统性能评估模型的研究与实现
Research and Implementation of a Model for Evaluating Performance of Replication Sub-system
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数据复制子系统的主要功能是完成异构数据的复制功能。
The function of data-copy subsystem is exchange data of different construction .
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放线菌线型复制子新的生物学功能及其应用
Novel Functions of the Actinomycetales Linear Replicons and its Applications