复制型

周围血白细胞的复制型丙型肝炎病毒RNA的检测及临床意义

Detection of replicative form of HCV RNA in peripheral blood leukocytes and its clinical significance

将貂肠炎病毒中间复制型DNA（MEVRFDNA）的HindⅢ酶切C片段克隆到pBR332中，形成重组质粒pBM。

Hind ⅲ C fragment of replicative form ( RF ) DNA of mink enteritis virus ( MEV ) was cloned into plasmid vector pBR322 , and which designated as pBM .

复制型DNA疫苗的研究进展

Replicative DNA vaccine : research advances

以非复制型HIV为载体进行目的基因转移的实验研究

Study on High-efficiency Gene Transfer of Pseudotyped HIV Vector

人呼吸道合胞病毒F蛋白非复制型重组腺病毒的构建与鉴定

Construction and Identification of Replication Deficient Recombinant Adenovirus Expressing F Protein of Human Respiratory Syncytial Virus

表达流行性感冒病毒HA基因非复制型重组腺病毒的构建及免疫效果的研究

Construction of Replication-defective Recombinant Adenoviruses Expressing Influenza Virus Hemagglutinin and Study on its Immune Responses

表达猪瘟病毒E2蛋白的复制型水疱性口炎假病毒的拯救

Recovery of Vesicular stomatitis virus Recombinants Expressing E2 Protein of CSFV

细菌内同源重组法构建HBVs区和C区基因非复制型腺病毒载体及其体外表达

Construction of replication-deficient recombinant adenoviral vector carrying HBV S and C region gene by homologous recombination in bacteria and its expression in vitro

不对称PCR制备单链探针检测登革Ⅱ型病毒复制型RNA和复制中间体RNA

Detection of dengue virus replicative intermediate and replicative form RNA with single strand DNA hybridization probes generated by asymmetric PCR

本文就复制型DNA疫苗的构建、作用机制、优点以及最新的研究进展作一简要综述。

In this article , the construction , mechanism of action , advantage and some new advances in research on replicative DNA vaccine are briefly reviewed .

本文采用非复制型脓病毒载体，利用同源重组方法，获得表达G蛋白多肽的非复制型重组腺病毒。

We used the replication - defective adenovirus vector and the homologous recombination , so we obtained the recombinant adenovirus by carrying the G-protein polypeptide .

一株呼吸道合胞病毒融合糖蛋白F和EGFP共表达的非复制型腺病毒重组体的构建、鉴定及表达

Construction and Identification of Replication-defective Recombinant Adenoviruses Which Co-express the Fusion Protein of RSV and EGFP

该复制型HBV转基因小鼠模型为研究HBV的发病机制研究以及抗乙肝药物开发研究提供了良好的实验动物模型。

The replicative HBV transgenic mice had provide an ideal animal model for the study on nosogenesis of HBV and anti-HBV drugs .

选择性杀伤CEA阳性结直肠癌细胞的条件复制型腺病毒载体的构建及应用

Construction and application of conditionally replicative adenovirus for selective cytotoxicity in CEA positive colorectal cancer cells

高复制型慢性乙型肝炎血清HBV复制指标与肝脏病变关系的探讨

Discussion on the Relationship between the serum HEV Replication Marks and Hepatic Pathological Changes in the Chronic Hepatits B of High Replication Type

庚型肝炎病毒作为复制型基因治疗载体及小干扰RNA对病毒表达复制抑制作用的研究

Studies on Hepatitis G Virus as a Replicable Vector for Gene Therapy and Inhibition of Gene Expression and Viral Replication by Small Interfering RNA

选择性复制型腺病毒介导的自杀基因HSV-tk在肿瘤基因治疗中的应用

The application of conditionally replication adenovirus-mediated suicide gene HSV-tk in the tumor gene therapy

结论携带抗-HBcScFv基因的非复制型重组腺病毒能在真核细胞中有效表达抗-HBcScFv，并具有特异性抗原结合活性。

Conclusion Anti-HBc ScFv can be expressed in cells mediated by recombinant replication defective adenovirus carrying the anti-HBc ScFv gene .

GPV是细小病毒科、细小病毒属成员，属自主复制型细小病毒。

GPY has been classified as a member of the parvovirus genus of the parvoviridae .

本试验就是利用这一特性构建了复制型重组CELO病毒载体，另外根据fiber1的C端抗体结合区Knob-s区进行了原核表达。

Based on this character , a replication adenovirus vector was generated . And the fiber 1 carboxy - terminal knob - s was expressed in E.

复制型DNA疫苗是基于常规DNA疫苗和RNA复制子疫苗的基础上发展起来的一种新型疫苗，既具有稳定性好、安全性高的优点，又实现了外源基因的高效表达。

Replicative DNA vaccine , based on conventional DNA vaccine and RNA replicon-based vaccine , is a kind of new vaccine . It not only has high stability and security , but also has high level of heterologous gene expression .

据报道，编码P1和病毒蛋白酶3C的非复制型DNA疫苗可以诱导产生抗病毒免疫应答。

They comprise the viral capsid named P1 . The non-replicating DNA vaccine , encoding P1 and the processing ( 3C ) proteinase , was reported to produce detectable antiviral immune response .

共表达HPV16L1、L2、E6、E7蛋白的重组非复制型痘苗病毒构建及鉴定

Construction and identification of non-replication recombinant vaccinia virus co-expressing human papillomavirus type 16 L1 / L2 / E6 / E7 proteins

肿瘤选择性复制型腺病毒（Conditionallyreplicationadenovirus，CRAds）可以特异性的在肿瘤细胞中复制，在复制的同时所携带的治疗基因也大量表达。

Conditionally replication of adenovirus in tumor tissue leads to amplification of the input dose at the tumor site , while the adenovirus-mediated gene can also largely replicate .

目的研究白细胞介素6（IL6）在非复制型痘苗病毒中的表达及其对重组痘苗病毒免疫效果的影响。

Objective To investigate expression of IL 6 in non replicating vaccinia virus and its immune effects on recombinant virus .

携带XAF1的复制型溶瘤腺病毒治疗恶性肿瘤的研究肝癌选择性溶瘤腺病毒的构建及其体外抑瘤作用

Construction of conditionally replication-competent adenoviral vectors targeting hepatocarcinoma and their in vitro anti-tumor effect

本研究成功构建了表达猪瘟病毒E0基因的非复制型重组腺病毒，该重组病毒免疫小鼠可产生较高的抗体滴度，免疫猪后能提供一定的保护效果。

The replication-defective recombinant adenovirus expressing ( CSFV ) E0 was constructed and could elicit favorable immune responses in mice , and offered protection for pigs to CSFV .

结论表达甲型流感病毒PB1的复制型DNA疫苗能保护小鼠抵御同型和异型流感病毒的攻击，本实验为流感疫苗研究提供新的候选抗原。

Conclusion The mice immunized by the replicative DNA vaccine encoding PB1 protein of influenza A can survive by the challenge of the homologous and heterologous influenza virus . A new alternative antigen of the experimental influenza vaccine is promising and worth to be further studied .

利用表达EBVgp350/220的非复制型重组痘苗病毒VMAΔCK及复制性重组痘苗病毒VMA，注射乳鼠脑内，观察毒力。

The non ? replicating vaccinia recombinant virus VMA Δ CK and the replicating virus VMA which all expressing the major envelope antigen ( MA ) GP350 / 220 of EBV were inoculated in the brains of the newborn mice to evaluate the virulence of them .

人轮状病毒抗原非复制型重组腺病毒黏膜免疫效果的研究

Mucosal immune response induced by recombinant adenoviruses expressing human rotavirus genes