分裂间期

背景与目的：评估肿瘤组织分裂间期的AgNOR定量分布已被广泛应用于肿瘤的诊断、疗效判断和预后评估。

BACKGROUND & OBJECTIVE : Evaluation of the quantitative distribution of interphase AgNOR ( argyrophilic nucleolar organizer regions ) has been widely used for diagnostic and prognostic purposes of tumor .

小麦细胞分裂间期外源染色质的检测

Detection of Alien Chromatins Introgressed into Wheat in Mitotic Interphase ALBUMINOUS CELLS

结论在细胞分裂间期，细胞核基因的检测中，与间期核FISH相比，DNAfiber-FISH分辨率更高。

Conclusion : Compared with interphase FISH , the resolution of DNA Fiber-FISH was higher in detecting genes on interphase cells .

被标记的细胞绝大部分是分裂间期，少数处在有丝分裂状态。

The more the labeled cells are in the interphase state ,, the more the mitosis occur .

目的为了提高细胞分裂间期细胞核基因检测的分辨率，以协助临床诊断。

Objective : To improve the detecting resolution of genes on interphase cells for assisting in clinical diagnosis .

但是目前有多篇报道称由于染色体重塑异常、癌基因扩增等原因可以导致细胞在分裂间期形成微核。

However , MN were also reported to form during interphase , due to disruptions in chromatin remodeling , or oncogene amplification .

用小鼠整条Y染色体特异探针涂染棕色田鼠的骨髓细胞中期分裂相和间期核，以小鼠骨髓细胞中期分裂相和间期核作为对照。

Metaphase chromosome and interphase nucleus of marrow cells of Microtus mandarinus were painted with the whole Y chromosome specific probe of mouse , and the metaphase chromosome and interphase nucleus of mouse marrow cells was used as the control .

为了快速检测特异性染色体，采用引物原位标记和细胞化学技术，以7号，17号，X和Y染色体特异性寡核苷酸作引物，对外周血淋巴细胞分裂中期染色体和间期核进行定位研究。

In order to identify specific chromosomes rapidly , with specific primers for chromosome 7 , 17 , X and Y , both metaphase and interphase nuclei of peripheral blood lymphocytes were detected by primed in situ ( PRINS ) labeling technique .

结果表明：一定浓度的亚硒酸钠（Na2SeO3）可导致蚕豆根尖细胞有丝分裂指数下降，间期细胞微核频率明显增高，且Na2SeO3的上述效应具有一定的剂量效应关系；

The results indicated that Na_2SeO_3 at a certain concentration could cause the mitotic index of the root-tip cells of Vicia faba to decline and the micronucleus frequency at the mitosis interphase to significantly increase , and these effects related to its dosage to some extent ;

结果：MPF活性在分裂期升高，在分裂间期下降。

Results : MPF activity increased during M phase and decreased during interphase .

采用改良吉氏染色法观察体外培养的阴道毛滴虫分裂相。结果显示，分裂间期为66.5%，二分裂期为24.1%，多分裂期为9.4%。

The observation showed that the percentage of Trichomonas vaginalis trophozoites at the stages of interphase , binary fission and multiple fission was66.5 % , 24.1 % and9.4 % respectively .