基因文库

大容量人免疫球蛋白G基因文库的构建及初步应用

Construction and preliminary use of a large human IgG gene library

应用随机PCR技术制备靶基因文库随机片段

Preparation of the random fragments of target gene library using random PCR technology

恶性疟原虫基因文库构建及特异DNA探针的应用研究

Construction of Plasmodium Genomic Library and Applications of the Specific DNA Probe

从小鼠胎儿基因文库中筛选分离免疫球蛋白重链Cε基因

Screening and Isolating of Mouse Immunoglobulin Heavy Chain C ε Gene from New Born Mouse DNA Library

中国人线粒体DNA基因文库的构建

The construction of Chinese mitochondrial DNA library

碱性土壤基因组DNA的分离纯化和基因文库的构建

Extraction and purification of DNA from alkalescence environmental samples and construction of mixed genomic DNA libraries

免疫核糖核酸基因文库构造&免疫核糖核酸基因cDNA合成

Construction of immune RNA gene library : synthesis of immune RNA gene cDNA

瘤胃中小韦荣球菌H2基因文库的构建

Construction of genomic DNA library for Veillonella parvula H2 in rumen

通过构建优质、道地、珍稀中药材DNA分子标记与基因文库，实现中药的防伪；

Realizes the Chinese herbal medicine ′ s anti-counterfeit through structuring the premium , genuine and rare traditional Chinese medicine ′ s DNA mark and gene library ;

因此，随机序列通过PCR重聚就可以得到一个能够编码大量蛋白质的一个随机基因文库。

Therefore , the random sequences could form a large-capacity and diversity gene library which coding large number of proteins by reassembly PCR .

在基因文库中任意选择53个克隆，利用专一的PCR进行DNA扩增以及dig非同位素标记，用于和草菇基因组DNA杂交。

Fifty three random clones from the genomic library were non - radioactivity digoxigenin - labeled by specific PCR and characterized by Southern hybridization .

志贺氏菌属弗氏2a染色体基因文库的构建

Construction of Genomic Library of Shigella flexneri 2a Chromosome DNA

新生大鼠脊髓损伤后再生相关基因文库构建及EST筛选

Clones screening and sequence identification of regeneration relation gene in newborn rats by suppression subtractive hybridization

16SRRNA基因文库对水基泥浆降解菌群的分析

Analysis on Bacterial Community Degrading Water-based Drilling Fluid by 16S rRNA Gene Library

泡桐基因文库的构建及actin基因同源顺序的亚克隆

Construction of Paulownia genome library and subcloning of the special DNA fragment homologous to actin gene

为了水稻线粒体基因文库的构建和细胞质雄性不育分子机制的研究，用简化的方法提纯了水稻线粒体DNA，限制性内切酶酶切图谱显示了清晰的带型。

In order to construct rice mitochondrial genome library and to study the molecular mechanism of Cytoplasmic Male Sterility , we isolated and purified rice mitochondrial DNA with a simplified method .

去势SD大鼠骨髓细胞粘附分子的表达及其与正常大鼠骨髓单个核细胞差异表达基因文库的构建

Construction of Differential Expression Gene Library between Normal SD Rat & Ovariectomy Rat and Expression of Cell Adherent Molecule in Rat 's Bone Marrow

本论文采用以PCR技术为基础的构建基因文库的方法对长江口沉积物反硝化细菌的nirS基因的多样性进行了研究。

The diversity of denitrifying bacterial nirS gene from the sediments of Changjiang Estuary was investigated by using a PCR-based cloning approach .

利用消减抑制杂交技术构建MPTP诱导的C57BL小鼠全脑差异表达基因文库

Construction of MPTP-induced differentially expressed gene librarys of C57BL mice brain by suppression subtractive hybridization

方法：利用噬菌体表面呈现技术，构建基因文库，经过panning筛选富集后，用ELISA方法检验抗原结合活性。

METHODS : The ScFv gene library was constructed by phage display technology . The affinity to antigen was detected by ELISA after panning .

Frankia基因文库的构建及与根瘤菌结瘤基因区同源克隆的筛选

Construction of Frankia Genomic Libraries and Isolation of Clones Homologous to Modulation Genes from Rhizobium leguminosarum

4X双二倍体在小麦染色体组分析中的利用志贺氏菌属弗氏2a染色体基因文库的构建

THE UTILIZATION OF 4X AMPHIDIPLOIDS IN THE GENOMIC ANALYSIS OF WHEAT Construction of Genomic Library of Shigella flexneri 2a Chromosome DNA

用λgt11载体构建人脑胶质瘤基因文库

Construction of the Human Glioma cDNA Library Using λ gt11 Vector

利用表达载体λgt11构建鸡毒支原体（MG）S6株基因文库。

A genomic library of Mycoplasma gallisepticum ( MG ) strain S6 DNA was generated by using bacteriophage lambda λ gt11 as the vector .

PyrococcushorikoshiiOT3基因文库的构建和新酶的筛选

Construction of a Genomic DNA Library of Pyrococcus Horikoshii OT_3 and Screening of the Novel Enzymes

当构建的内江水体16Srdna基因文库中测序克隆数约为100个时多样性估算达到最大值。

The research result showed that the species richness estimators reached the maximum when the number of clones sequenced in bacterial 16S rDNA library of Neijiang water body was about 100 .

经测定包装滴度为5.23×10~6pfu／ml，蓝白斑筛选重组率为96.8%，表明已成功构建血清1型RA基因文库。

The results showed that the packaging titer was 5.23 × 10 ~ 6 pfu / ml and recombinant rate was 96.8 % . It is concluded that the constructed genomic library can be used to screen target clones .

为了解富营养、氮素污染严重的胶州湾海域反硝化细菌群落的结构与组成，应用PCR技术为基础的基因文库方法对胶州湾沉积物反硝化细菌nirS基因的分子多样性进行研究。

To understand the composition and structure of denitrifying communities in the Jiaozhou Bay , the diversity of denitrifying bacterial nirS gene from the sediments of Jiaozhou Bay was investigated by using a PCR-based cloning approach .

运用λgt11噬菌体构建人脑胶质瘤基因文库的实验研究

Experimental studies on the construction of a human glioma cDNA library using λ gt11 vector

以此片段为探针分别筛选水稻根cDNA文库及水稻基因文库，获得一条全长cDNA及其相应的全长基因，并通过测序得到了两者的全序列。

Using the PCR fragment as the probe for screening , a complete cDNA and its complete gene were obtained from that cDNA library and a rice gene library , respectively . Both of the complete cDNA and the complete gene were sequenced .