培养瓶

约2周后，细胞培养瓶底可形成一单层细胞，呈集簇状生长。

About 2 weeks later , a unilaminar cells formed at the bottom of culture flask , presenting cluster-shape .

7-9天左右细胞可长满培养瓶底，达95%以上融合，主要呈长梭形，其中散在少量折光性强的小圆形细胞。

About 7-9 days later , cells might overgrow the bottom of culture flask and reached over 95 % fusion .

重组质粒DNA的浓度为8μg/100mL～10μg/100mL培养瓶；

The concentration of the recombinant plasmid DNA is about 8 to 10 μ g per flask ;

一次性塑料培养瓶为Orange公司产品。

Disposable plastic culture flasks were purchased from Orange .

方法：①采用美国BD原装培养瓶及BD9050全自动血培养仪，进行血培养。

Methods : Infant blood samples were cultured using the bottle produced by BD company and automated blood culture instrument of BD9050 ;

中止消化后，在100g条件下离心5min，收集细胞种入25cm2细胞培养瓶。

After terminating the digestion , centrifuge the solution at 100 g for 5 min , and seed the cell into a 25 cm2 vented cap flask .

方法利用15L培养瓶旋转培养Vero细胞，观察不同培养条件下，对细胞形态及病毒释放的影响。

Methods : Rotating Vero cells culture was done within 15L cultivation flask and the effect of cell form and virus discharge was observed under different culture conditions .

取出生1-3天的SD大鼠15只，无菌取其心室肌用0.25%胰蛋白酶消化后制成心肌单细胞悬液，移入培养瓶进行培养。

15 newborn Sprague-Dawley rats of 1-3 days age were prepared . The ventricles muscle were take out to make into myocardial cells with no germ after they were digested with 0.25 per cent of trypsinase . The myocardial cells were moved into the culture bottle to culture them .

方法：应用不完全弗氏佐剂诱导小鼠腹腔淋巴管瘤形成，消化法分离获得LEC，置于自制的鼠尾胶包被的培养瓶（板）中培养。

METHODS : Mouse lymphangiomas in abdominal cavity were induced by incomplete Freund 's adjuvant , then disrupted and digested to obtain LEC , which were cultured in the flask or plate previously coated with rat-tail collagen .

传代细胞24h内完全贴壁，伸展成梭形，开始迅速增殖，7天即铺满培养瓶底，传代细胞保持原代细胞的形态特征。

After 24 hours , the cells of passage adhered to the plastic surface , extended to become shuttle-shape and began to proliferate rapidly . After 7 days , the cells overspread the culture bottle bottom , and kept the same figure characteristics of primary culture .

有核细胞1×10~6与采自健康供者髂骨的相同数量的骨髓细胞分别接种于25cm~2培养瓶，14d后计数成纤维细胞集落（CFU-F）。

At the same time , 1 × 10 ~ 6 nucleated BM cells and same amount of nucleated cells from iliac aspirate were seeded in 25 cm ~ 2 tis - sue flasks , colony forming unit-fibroblast ( CFU-F ) assay .

目的：评价Hemoline双相血培养瓶临床应用情况。

Objective : To evaluate the application of double blood culture bottle .

伯泰血培养瓶对细菌培养生长能力和敏感性分析

Analysis of bacteria growth ability and sensitivity against Be-tai blood culture bottle

双相血培养瓶的临床应用效果分析

Effect analysis of biphasic blood culture bottle applied in clinic

非洲菊离体培养瓶内诱导生根的研究

The Study of Root Induction in Vitro Vessel of Gerbera

使用带毛玻璃塞子的培养瓶。

Incubation bottles with ground-glass stoppers are utilized .

计数10~6鸡胚颅盖骨成骨细胞样细胞于培养瓶内培养，共10瓶。

The isolated osteoblastic cells from embryonic chicken calvaria bone were cultured in vitro .

培养瓶可以中和一定浓度的抗生素。

And the antibiotics in the specimens could be neutralized by the culture bottle .

在运输之前，细胞从培养瓶壁上消化下来后冻存。

Prior to shipping , cells are detached from flasks and immediately cryo-preserved in vials .

实验室用玻璃长颈瓶（例如蒸馏瓶、培养瓶、商量容量瓶）

Flask of glass for laboratory use ( e.g. , distilling , culture , volumetric )

双相血培养瓶临床应用探讨

The application of double blood culture

适宜的表皮细胞接种密度为2×106cell/75cm2培养瓶。

And the proper seeding concentration is 2 × 106 cell / 75cm2 flask in primary culture .

该系统包括一直角座标机器人、机器人手端、培养瓶定位装置总成、以及控制系统。

This system consists of a Cartesian robot , robotic end-effector , flask stand assembly , and control system .

一种双相培养瓶，属于细菌培养器具技术领域。

The utility model relates to a biphase culturing bottle belonging to the technical field of a bacteria culture appliance .

在工厂化组培苗生产过程中，为减少病菌污染，生产上多采用细口培养瓶育苗。

In the process of tissue culture plantlet production , the plantlet bottle with small entrance is used for reducing bacteria pollution .

首次对培养瓶育苗方式条状组培苗的自动化生产技术进行了研究。

The followings are the main research findings : 1 . Automatic product technique of tubular tissue culture plantlet in plantlet bottles is studied for the first time .

实验以油田污水中的上述三种细菌为主要研究对象，采用细菌培养瓶法和试管稀释法对三种细菌进行了培养和计数。

Above three kinds of bacteria of oilfield sewage were the main research objects , a bottle of law and culture in vitro dilution of the three bacteria were cultured and counting .

悬浮细胞随着换液次数的增多而逐渐被去除，约7～10天左右，细胞融合成单层，长满整个培养瓶。

With the cell suspension for an increase in the number of gradually being removed , about 7 to 10 days , a single cell fusion , covered the entire culture bottles .

用圆柱形细胞培养瓶，对草鱼吻端组织ZC&7901细胞适应旋转培养条件进行了研究，测定了细胞接种浓度、旋转速度及细胞生长速度等有关参数。

A new method was developed for rotation culture of ZC-7901 cells , a cell line from grass carp snout . The culture condition such as cell inoculation concentration , rotation speed and cell growth rate were studied .

结果手工血培养瓶阳性检出率为10.28%，手工使用中和抗生素培养瓶阳性检出率为20.83%，两者差异有统计学意义（P<0.01）；

RESULTS The bacteria detected rates were 10.28 % in manual blood-culture bottles and 20.83 % in antibiotic-neutralized culture bottles applied by manual operations . There was significant difference between both of them ( P < 0.01 ) .