四氮唑

对玉米生活力测定方法进行了研究，得到最佳的测定条件为：红四氮唑（TTC）浓度为05%，加热温度45℃，加热时间为10min。

The detecting method for vigor of maize was studied . The optimum conditions were as follows : TTC concentration 0.5 % , heating temperature 45 ℃, heating time 10 min.

用红四氮唑染色法测定各组心肌梗死面积，TUNEL法检测心肌细胞凋亡情况，同时测定血清肌酸激酶（CK）活力和血清肌钙蛋白T（TnT）水平。

TTC staining method was used to determine the myocardial infarct size . Cardiac myocytes apoptosis was detected by TUNEL . The activity of creatine kinase ( CK ) and concentration of troponin T ( TnT ) in serum were measured simultaneously .

氯化硝基四氮唑蓝共振散射法检测DNA

Determination of Deoxyribonucleic Acid with Nitro - Tetrazolium Chloride Blue by Resonance Light-Scattering Technique

四氮唑盐（MTT）比色法检测细胞的增殖；

CFs proliferation was measured by thiazolyl blue ( MTT ) assay ;

四氮唑盐（MTT）法检测半数细胞抑制浓度。

Results MTT method was used to evaluate the 50 % inhibition concentration .

四氮唑及2（1H）-喹啉酮类含氮稠杂环化合物及其衍生物是重要的医药中间体，其药物广泛应用于心血管类疾病的治疗。

Tetrazole and carbostyril and their derivatives are widely applied as cardiovascular drugs .

目的介绍一种用四氮唑蓝（MTT）为底物的酶反应定量比色分析法。

Objective To introduce a quantitative colorimetric assay based on a tetrazolium salt ( MTT ) .

应用四氮唑蓝比色（MTT）法检测各孔的吸光度值并计算出肿瘤细胞对所试药物的敏感率。

The absorption values of 96 well were detected and the sensitive rates were calculated with MTT methed .

方法：从海胆肠内提取水溶性多糖类成分。应用四氮唑蓝（MTT）快速比色法测定肿瘤细胞生长抑制率。

Methods The inhabited rate ( IR ) of cell growth was determined with the MTT colorimetric assay .

通过神经功能评分和氯化三苯基四氮唑（tetrazoliumchloride，TTC）染色对模型进行评价。

The model was evaluated by neurology score and tetrazolium chloride ( TTC ) stain .

研究了氯化硝基四氮唑蓝与脱氧核糖核酸在酸性条件下的共振光散射特征，考察了影响因素和最佳反应条件，建立了一种测定DNA的新方法。

Characteristics of the interaction of nitro-tetrazolium chloride blue and Deoxyribonucleic Acid ( DNA ) were studied in the of pH 1.8 buffer solution by Resonance Light-Scattering ( RLS ) technique .

本文研究了1-苯基-5-巯基四氮唑（PMT）光降解过程。

The photodegradation of phenylmercaptotetrazole ( PMT ) was studied in this paper .

心肌切片氯化硝基四氮唑蓝染色（NBT），判断心肌梗死面积（IS）。

Infarct size ( IS ) was estimated by NBT staining .

分别采用四氮唑蓝法和生物发光法测定各组大鼠血红细胞SOD活性及心肌ATP含量。

SOD activities in blood RBC and the myocardial ATP contents of rats in each group were determined respectively with NBT and bioluminescence methods .

方法用硝基四氮唑蓝法研究硫酸软骨素对体外培养兔软骨细胞琥珀酸脱氢酶（SDH）活性的影响。

Methods The effect of chondroitin sulfate on the succinate dihydrogenase ( SDH ) of chondrocytes were measured by nitroblue tetrazolium method .

方法形态学观察和硝基四氮唑蓝还原反应鉴定DADS诱导HL-60细胞分化；

Methods Cell differentiation was validated by morphological method and nitroblue tetrazolium ( NBT ) reduction test .

硝基四氮唑蓝（NBT）还原实验检测细胞向单核/巨噬系统分化。

Cell differentiation was measured by nitro blue tetrazolium ( NBT ) reduction test .

目的：确立2,3,5-三苯基氯化四氮唑（TTC）在微生物限度检查菌落计数中应用的适宜浓度。

Objective : To establish the suitable concentration of TTC in the colony count of microbial limit tests .

用硝基四氮唑蓝（NBT）还原试验定量测定HL60细胞的机能分化；

Functional differentiation of HL 60 cells was examined with NBT reduction test .

方法：高效液相色谱仪分析氨基酸，氯化硝基四氮唑蓝（NBT）光化还原法测定SOD的含量。

Methods : The contents of amino acids were analyzed by high performance liquid chromatography and SOD was determined by nitroblue tetrazolium photoreduction method .

方法：用四氮唑蓝（NBT）定量法测定新生儿红细胞G-6-PD活性。

Methods : NBT was used to detect activities of G - 6 - PD .

用四氮唑蓝比色法（MTT法）检测不同剂量和时间时SPC-A1细胞的存活率。

The SPC-A1 cell survival rates of different groups were detected by methyl thiazolyl tetrazolium chromatometry assay ( MTT assay ) .

方法：采用溴化四氮唑兰法（MTT法）研究了19例初治急性髓系白血病患者骨髓原代白血病细胞和K562细胞系体外对7种化疗药物及两药联合使用的敏感性。

Methods : 19 primary acute non lymphatic leukemia patients and K562 cell line ′ s chemosensitivity to 7 kinds of drugs were evaluated with MTT assay in vitro .

本文应用显微组织化学鉴定的方法，氯化三苯四氮唑（TTC）法，对寄生植物肉苁蓉种子的活力进行测定并对TTC法的测定方法和测定条件进行了研究。

In this paper we reported the study on determination of seed vigor of Cistanche deserticola Ma by the Cyto-chemical methods and TTC-test .

利用嗜热链球菌为菌种检验牛奶中抗菌素残留的氯化三苯基四氮唑法（TTC法），是我国的国家标准方法。

The TTC method which uses Streptococcus Thermophilus as test bacteria is a standard method to detect antibiotics residues in milk in China .

用氯化硝基四氮唑蓝（NBT）还原法测酵母多糖A刺激大鼠中性白细胞产生的O2.；

Superoxide ( O_2 ~ (( . ) )) from zymosan-stimulated neutrophils of rat was measured by NBT reduction method .

用四氮唑蓝法测定姜黄素组、肿瘤坏死因子相关凋亡诱导配体组以及联合组的吸光值，根据吸光度值计算肺癌细胞株A549细胞生长抑制率。

The absorbance values of the curcumin group , TRAIL group and combination group were determined by MTT method to calculate the inhibitory rate of A549 cells .

目的探讨MTT比色法在化疗药物敏感试验中的适宜条件。用四氮唑盐比色法（MTT）检测，计算细胞相对增殖度，用六级毒性分类法评级。

Objective To investigate the methodology of tetrazolium based colorimetric assay ( MTT assay ) in chemosensitivity test of chemotherapy . The proliferation of the cells was examined using tetrazolium colorimetric assay .

用联苯胺染色法和氯化三苯基四氮唑（TTC）染色法测定百山祖冷杉花粉活力，均未取得满意的结果，改用培养基培养法能得到较为满意的结果。

The pollen vitality could not be mensured by benzidine staining method and TTC staining method . But it could be mensured by the method of culture medium .

分别对实验组及正常对照组实施静息、腺苷负荷心肌声学造影并采集存图，造影结束后行2，3，4-三苯基氯化四氮唑（TTC），伊文思蓝心肌组织染色。

On the experimental group and normal control group , the rest and adenosine stress myocardial contrast echocardiography was performed , and followed TTC , evans blue staining .