囊胚

目的：确定囊胚期胚胎的最佳种植时期，提高胚胎移植率。

Ve : To determine the optimal time for blastosphere implantation in order to improve the survival rate of embryonic implantation .

近交系小鼠体细胞核移植囊胚的微卫星DNA鉴定

Microsatellite DNA analysis of inbred mouse somatic cell nuclear transfer blastocysts

经统计分析，早期囊胚的存活率显著高于桑椹胚（P＜0.05）。

The survival rate of early blastocysts was significantly higher than that of the morula ( P0.05 ) . 6 .

颗粒细胞共培养能显著的提高囊胚发育率，囊胚率达39.1%（P＜0.05）；

D cumulus co-culture is significantly capable to support the development of bovine embryos to blastocysts rate of 39.1 % ( P < 0.05 ) .

维生素A酸对小鼠囊胚Fas蛋白表达的影响

Effect of Retinoic Acid on the Expression of Fas Proteins in Mouse Blastocysts

从胚胎培养开始就添加葡萄糖可提高胚胎的囊胚发育率（20.1%vs10.3%，P＜0.05）。

Culturing embryos with glucose from the beginning of culture improved the blastocyst formation rate significantly ( 20.1 % vs 10.3 % , P < 0.05 ) .

将3株诱捕ES细胞分别经显微注射引入到受体囊胚中，再植入假孕母鼠的子宫中使其发育成小鼠。

ES cells from 3 trapped lines were introduced into blastocysts by microinjection .

与单个胚胎培养组相比较，三个胚胎培养组的优质囊胚形成率增高（P0.05）。

Compared with single embryo culture group , three embryo group cultivation increased quality blastocyst formation rate ( P0.05 ) .

ECG能提高囊胚率，可以促进胚胎囊胚的发育，但并无统计学差异。

ECG can improve the blastocyst rate , blastocyst embryos can promote the development , but no significant difference .

IGF-1对体外培养囊胚细胞抗凋亡的作用

Anti-apoptotic effect of insulin-like growth factor 1 on blastocysts cells in vitro

结论高浓度葡萄糖对小鼠囊胚有毒性作用，可诱导小鼠囊胚细胞的凋亡，Caspase-3参与了高浓度葡萄糖诱导囊胚细胞的凋亡作用。

Caspase-3 is involved in the apoptosis in mouse blastocysts induced by high glucose .

9个核移植重构囊胚中分离出ES细胞集落，分离率为2.77%；

ES cell colonies were isolated from 9 blastocysts reconstructed , isolation rate was 2.77 % .

脂质体量为4μg，质粒DNA量为3μg时，转染白来航鸡原代囊胚细胞的效率最高。

The transducting efficiency of White Leghorns primary blastodermal cells is highest when the liposome amount is 4 μ g and plasmid DNA amount 3 μ g.

囊胚细胞为供体的嵌合体制作：分离了X期的麻鸭囊胚细胞，进行了体外培养，发现在培养基中添加2-3%的胚胎提取物能很好的促进囊胚细胞的体外生长和增殖；

We found that the 2-3 % of embryonic extracts in DMEM is more suitable for the growth and proliferation of blastodermal cells in vitro ;

Cox-2在囊胚之前的各个时期胚胎均未检测到，而在扩展胚泡和孵出胚泡中表达，Cox-2的时期特异性表达可能与兔胚泡孵出及胚胎着床等过程相关。

The stage specific expression of Cox 2 may be related to embryo hatching and implantation .

小鼠囊胚的不同遗传背景对形成ES细胞集落的影响

The Effects of Different Genetic Background of Mouse Blastocysts on the Formation of Embryonic Stem Cell Colonies

获得93%的48h发育率和48%的囊胚率，显著高于低渗处理前未培养组（47%和9%）（P＜0.05）。

Cultured embryos obtained higher development rates ( 93 % ) and blastocyst rates ( 48 % ) than uncultured , significantly .

猪ICM注人囊胚获得嵌合胚的研究

Study on Chimeras of Blastocyst Injection of ICM in Pig

用离子霉素联合6-DMAP能有效地激活绵羊卵母细胞，其囊胚率为30.4%，高于细胞松弛素B法的27.4%；

Matured sheep oocytes were efficiently activated by Ionomycin together with 6-DMAP and 30.6 rate of blastocyst were achieved .

通过反相高效液相色谱法（HPLC）分析小鼠植入前胚胎体外培养各个时期培养基中氨基酸浓度变化；3、研究了氨基酸在不同囊胚率变化情况。

Through reversed-phase high performance liquid chromatography ( HPLC ) analysis of mouse preimplantation embryo in vitro culture medium in each period changes in amino acid concentration .

但在融合后的桑椹胚/囊胚形成率上，成纤维细胞又明显低于卵丘颗粒细胞和输卵管上皮细胞（P0.05）。

However , the morulae / blastocyst formation rate of fibroblasts was significantly lower than cumulus granulosa cells and oviduct epithelial cells after fused ( P0.05 ) .

本试验先后将109枚胚胎用于此项研究，通过将小鼠ES细胞注入8-16细胞桑椹胚和囊胚，观察两个不同时期注射胚胎的发育能力。

This experiment collected 109 embryos to observe their developmental ability by ES cells microinjection into 8-16 cell stage morula or blastocyst .

结论BALB/C小鼠囊胚在原代小鼠胚胎成纤维细胞饲养层上可以发育成ES细胞。

Conclusions The blastocysts from BALB / C mice can be cultured on the PMEF feeder layers and developed into ES cells .

OPS一步法冷冻后囊胚发育率高达97.92%，与对照组（100%）无显著性差异（P＞0.05）。

While the blastocyst rate of vitrified mouse morula in the 1-step OPS method ( 97.92 % ) were similar ( P0.05 ) to that in controls .

卵裂期、囊胚早期、原肠中期胚胎在2~8℃水温下，处理3d后孵化率低于30%；

When exposed to 2 ~ 8 ℃ for 3 days , the hatching rate of embryo at its cleavage , early blastula , and mid gastrula stages was lower than 30 % .

用RPE冷冻器冷冻42枚桑椹胚和13枚囊胚。

42 morulae and 13 blastocysts were frozen in 1 M glycerol / PBS in the RPE freezer .

D3胚胎细胞数及质量分级与囊胚形成有密切关系。

The cell number of day 3 embryo and quality score have some indication to blastulation .

目的：分析囊胚培养及移植技术在体外受精-胚胎移植（IVFET）中的临床效果。

Objective : To study the clinical effects of blastocyst culture technique on the outcome of in vitro fertilization-embryo transfer ( IVF-ET ) .

目的观察胚胎干细胞（ES细胞）囊胚腔注射的方法构建嵌合体小鼠自发性肿瘤。

Objective To study the spontaneous tumors derived in chimeric mice resulting from injection of embryonic stem ( ES ) cells into host blastocysts .

探讨TSA不同处理时间对核移植重构胚的卵裂率和囊胚率的影响。

Investigated the effect of TSA different treatment time on the nuclear transfer embryo cleavage and blastocyst rate .