变性梯度凝胶电泳

利用变性梯度凝胶电泳、克隆和实时PCR等分子生物学技术对2个厌氧氨氧化反应器中的微生物进行了初步研究。

The molecule biological techniques DGGE , clone and real-time PCR were utilized to study prinimilarily the microorganism in2 anaerobic ammonia oxidation reactors .

变性梯度凝胶电泳装置及其在DNA突变检测中的初步应用

Denaturing gradient gel electrophoresis system used in mutations detecting

用变性梯度凝胶电泳分析PCR克隆的突变率

Analysis of mutant frequency of PCR cloning by denaturing gradient gel electrophoresis

变性梯度凝胶电泳检测到四例新的FⅨ基因突变

Four novel point mutations of factor ⅸ gene detected by denaturing gradient gel electrophoresis

设计了一套适用于变性梯度凝胶电泳（DGGE）的装置。

A denaturing gradient gel electrophoresis ( DGGE ) system was designed .

应用变性梯度凝胶电泳技术和16Srdna序列分析技术研究山羊瘤胃细菌的多样性

Bacterial Diversity in Goat Rumen as Characterized by Denaturing Gradient Gel electrophoresis and 16S rDNA Sequence Analysis

方法：用PCR、变性梯度凝胶电泳（DGGE）和DNA测序检测因子Ⅷ基因突变。

Methods : PCR , denaturing gradient gel electrophoresis ( DGGE ) and DNA sequencing were used to screen mutations in the factor ⅷ gene .

粪样细菌区系的变性梯度凝胶电泳（DGGE）分析；

Analysis of calves ' excrement with Denaturing Gradient Get Electrophoresis ( DGGE );

并采用分子生物学技术聚合酶链式反应&变性梯度凝胶电泳（POLYMERASECHAINREACTION-denaturinggradientgeleleetrophoresis，PCR-DGGE）对污泥微生物群落的演替展开研究。

The microbial community structures and succession were studied by polymerase chain reaction-denaturing gradient gel eleetrophoresis ( PCR-DGGE ) .

为此，在介绍变性梯度凝胶电泳（DGGE）以及荧光原位杂交（FISH）等分子生物学新技术的基础上，对这些技术在土壤微生态研究中存在的问题和发展前景做了展望；

In this paper , several molecular methods such as fluorescence in situ hybridization ( FISH ) and denaturing gradual gel electrophoresis ( DGGE ) etc.

变性梯度凝胶电泳分析PAH基因外显子3突变

Analysis of Mutations in Exon 3 of Phenylalanine Hydroxylase Gene from Southern Chinese PKU by Denaturing Gradient Gel Electrophoresis

本文还首次将非培养方法（变性梯度凝胶电泳，DGGE）应用于水稻植物内生细菌研究，进行了初步的探索。

A preliminary study on rice endophytic bacteria using culture-independent method ( DGGE ) was done in this paper .

DGGE（变性梯度凝胶电泳）技术目前被广泛应用于分析微生物的种群组成。

Denaturing gradient gel electrophoresis ( DGGE ) has been widely applied for study microbial population structures recent years .

包括变性梯度凝胶电泳实验、16Srdna克隆库、DNA生物芯片在内的分子生物学技术表明在自然环境中物种存在极端多样性。

Molecular techniques , such as denaturing gradient gel electrophoresis ( DGGE ), 16S rDNA clone library , and DNA microarray , reveal extraordinarily diverse species in the natural environment .

变性梯度凝胶电泳技术（denaturinggradientgelelectrophoresis，DGGE）是一种重要的分子多态性技术，在物种鉴定及浮游生物群落多样性研究方面有着广泛的应用。

Denaturing gradient gel electrophoresis ( DGGE ) is an important molecular polymorphism technique . It has been widely used in the study of species identification and plankton community composition .

主要试验结果如下：1.利用变性梯度凝胶电泳（DGGE）技术分析肠道总菌群结构的变化。

The technique of denaturing gradient gel electrophoresis ( DGGE ) was used to analyze the changes of total microflora structure .

通过直接提取土壤细菌DNA，运用PCR、变性梯度凝胶电泳和分子克隆等技术，分析土壤细菌16Srdna的多态性。

The 16S rDNA diversity of bacteria was studied by directly extracting bacteria DNA from mangrove soil and employing polymerase chain reaction , denaturing grade gel electrophoresis ( DGGE ) and clone technology .

基于16SRRNA基因的变性梯度凝胶电泳（denaturinggradientgelelectrophoresis，DGGE）技术具有直观、快速和高通量的优点，适合于分析复杂的微生物菌群。

Denaturing gradient gel electrophoresis ( DGGE ) targeting 16S rRNA-encoding genes was developed as rapid methodologies with high throughput that are more suitable for analysis of complex microbial communities .

利用PCR／DGGE技术，对土壤和叶面细菌总DNA16SrDNAV6可变区进行扩增，然后进行变性梯度凝胶电泳（DGGE），结果表明土壤、叶面的微生物群落表现出一定的多态性。

The 16S rDNA V6 region of soil and phyllosphere bacteria were amplified , and the result of DGGE ( Denaturing Gradient Gel Electrophoresis ) showed ecological diversity .

目的应用变性梯度凝胶电泳（DGGE）方法分析经牙周基础治疗使牙周袋变浅后龈下微生物种类的变化。

Objective To investigate the changes of subgingival bacterial communities in periodontal pockets with different depth using denaturing gradient gel electrophoresis ( DGGE ) analysis .

PCR（聚合酶链式反应）-DGGE（变性梯度凝胶电泳）技术能够很好地分离PCR产物中的具体基因，为在时间与空间上追踪优势菌株提供了新的方法。

PCR ( Polymerase Chain Reaction ) - DGGE ( Denaturing Gradient Gel Electrophoresis ) has been showed to be a powerful tool for separating specific genes from a PCR product and providing a culture-independent method for tracking dominant baterial populations in space and time .

我们应用了PCR-DGGE技术（聚合酶链式反应-变性梯度凝胶电泳技术）监测土壤微生物群落结构和遗传多样性。

We use the PCR-DGGE technology ( the polymerase chain reaction & degeneration gradient gel electrophoresis technology ) to test the soil microbial diversity and community structure information .

方法利用聚合酶链反应&变性梯度凝胶电泳技术对49例2型糖尿病合并CAD患者、49例未合并CAD的2型糖尿病对照者和101例健康对照者进行PON基因外显子筛查。

Methods The exons of PON gene were screened by polymerase chain reaction denaturing gradient gel electrophoresis in 49 type 2 DM patients complicated with CAD , 49 CAD uncomplicated type 2 DM as well as 101 healthy control subjects of Chinese population .

在本研究中，我们首先结合传统的变性梯度凝胶电泳（DGGE）、克隆文库技术与新兴的焦磷酸测序技术比较了长期注水的孤岛油田注水井和采油井中的微生物组成。

Using denaturing gradient gel electrophoresis ( DGGE ), 16S rRNA gene clone library and pyrosequencing , we compared the microbial communities of injection well with that of production well .

应用基于聚合酶链式反应（PCR）的变性梯度凝胶电泳方法（DGGE）分析了在高温、高压和厌氧条件下富集培养的油藏内源微生物16Srdna。

Denaturing gradient gel electrophoresis method ( DGGE ) based on polymerase chain reaction ( PCR ) was used to analyze the indigenous microorganism 16S rDNA that was enriched under high-temperature high-pressure and anaerobic conditions of oil reservoir .

对当前堆肥中微生物种群分布及其对有机物分解作用的研究进行分析，论述了分子生物技术中的变性梯度凝胶电泳（DGGE）的特点。

Studies on the distribution of microbe and their degradation of organic material in compost are presented , and the character of denaturing gradient gel electrophoresis ( DGGE ) method is also discussed .

采集了酸性矿山废水区域酸性沉积物样品，运用16Srdna克隆文库技术和变性梯度凝胶电泳（DGGE）技术，研究了沉积物中的嗜酸菌类群以及它们的群落结构。

A sediment sample was collected at an acid mine drainage site in Anhui . The acidophiles composition and community structure in the sediment was studied with 16S rDNA clone library and denaturing gradient gel electrophoresis ( DGGE ) .

方法以聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）和自动DNA分析检测p53基因5～8外显子的序列变化。

Methods Biopsies of 22 squamous cell carcinoma ( SCC ) and 18 basal cell carcinoma ( BCC ) were analyzed by polymerase chain reaction denaturing gradient gel electrophoresis ( PCR-DGGE ) and DNA sequencing for alterations in the exons 5 ~ 8 of p53 tumor suppressor gene .

采用常规化学分析和微生物群落变性梯度凝胶电泳（DGGE）监测技术，探讨投加不同价态铁元素对硫酸盐还原过程的影响以及相应的微生物群落动态响应。

The influence of different valence state Fe element on sulfate reduction process and the dynamic response of corresponding microbial community were explored by conventional chemical analyses and microbial community denatured gradient gel electrophoresis ( DGGE ) technique .

目的检测皮肤癌p53基因5～8外显子的突变频率，比较变性梯度凝胶电泳（DGGE）和单链构象多态性分析（SSCP）检测p53基因突变的敏感性。

Purpose To detect the mutation in the exon 5-8 of p53 gene in skin cancer by denaturing gradient gel electrophoresis ( DGGE ) and single strand conformation polymorphism ( SSCP ) . Comparing the sensitivity of DGGE and SSCP in screening mutations of p53 gene .