单链构象多态性分析

乏情不是一种疾病，而是一种多方面的体态迹象。临床常见病原菌16Srdna单链构象多态性分析

Anestrus ( absence of estrus ) is not a disease but a sign of a variety of conditions . 16S rDNA single-strand conformation polymorphism analysis of common clinical pathogenic bacteria

临床常见病原菌16Srdna单链构象多态性分析

16S rDNA single-strand conformation polymorphism analysis of common clinical pathogenic bacteria

进行基因位点DNA扩增聚合酶链反应-单链构象多态性分析。

Gene site DNA amplification was analyzed with polymerase chain reaction-single strand conformation polymorphism ( PCR-SSCP ) .

人肝癌组织中E-cadherin基因DNA单链构象多态性分析

SSCP analysis of E cadherin gene in human hepatocellular carcinomas

免疫荧光法与p53单链构象多态性分析检测胸水肿瘤细胞的结果分析

Analysis of tumor cells in thoracic liquid by fluorescence and p53 single-strand conformation polymorphism

HLA-A、B、C基因第二外显子的单链构象多态性分析及骨髓移植受供者基因配型

PCR-SSCP Analysis of Exon 2 of HLA-A , B and C Genes and Matching for Bone Marrow Donor-Recipient Pairs

单链构象多态性分析显示两病例组与对照组均出现3种不同的DNA带型，分别称为Ⅰ，Ⅱ，Ⅲ带型。

SSCP showed that 3 different DNA patterns , which were band patterns ⅰ, ⅱ, ⅲ respectively , were observed in all the 3 groups .

噬菌体生物扩增法、DNA序列分析及单链构象多态性分析检测结核分枝杆菌耐药性

Analysis and evaluation of phage amplified biologically assay , DNA sequencing analysis and single strand conformational polymorphism for the drug susceptibility testing of Mycobacterium tuberculosis

方法多聚酶链聚合反应单链构象多态性分析（PCRSSCP）、基因测序。

Methods Polymerase chain reaction - single strand confirmation polymorphism ( PCR-SSCP ), sequening .

方法运用双重对照PCR及单链构象多态性分析技术检测17例原发性膀胱癌。

Methods The status of MTS1 gene in17 cases of primary bladder cancer was detected by comparative multiple PCR and single-strand conformation polymorphism ( SSCP ) .

人类免疫缺陷病毒gag基因的单链构象多态性分析

Analysis on single-strand conformation polymorphism of human immunodeficiency virus ( HIV-1 ) gag gene

方法应用PCR单链构象多态性分析技术，对胃癌患者的门静脉血、外周静脉血分别进行K-ras、p53基因检测，术后随访半年，了解肝转移情况。

Methods By PCR-SSCP K-ras and p53 gene mutation in portal vein blood and peripheral blood were detected in 62 patients with gastric carcinoma .

用单链构象多态性分析（PCR-SSCP）对ING1基因进行突变检测；

Mutation analysis was detected by PCR-SSCP ( single-strand conformational polymorphism ) .

方法采用RT-PCR法（逆转录DNA聚合酶链反应）、PCRSSCP（聚合酶链单链构象多态性分析）、DNA序列分析法对26例鼻咽癌组织及癌旁组织标本进行polβ基因突变研究。

Methods Twenty-six cases of nasopharyngeal carcinoma tissues and control epithelial tissues were collected to study DNA pol β gene mutation with PT-PCR , PCR-SSCP and DNA sequence analyzing technique .

26Srdna单链构象多态性分析在临床酵母菌菌种鉴定中的应用

Rapid identification of clinical yeast species by single-strand conformation polymorphism analysis of 26S rDNA D1 / D2 domain

方法：采用常规酚抽提法从外周静脉血提取DNA，应用多聚酶链反应-单链构象多态性分析技术对被试者的早老素1基因第5号和第8号外显子进行突变检测。

METHODS : The conventional phenol extraction method was used to withdraw DNA from peripheral blood , and polymerase chain reaction-single strand conformation polymorphism analysis ( PCR-SCCP ) was applied to detect the mutation of the 5th and 8th exons in presenilin-1 gene .

进一步用DNA单链构象多态性分析（SSCP）技术对HCV基因变异进行研究，发现在同一家庭内的成员间HCVcDNA的单链构象一致，但不同家庭的成员间HCVcDNA的单链构象呈现多态性。

Comparison of SSCP patterns revealed that the SSCP of HCV cDNA was identical among members of the same family , but the single strand conformation of HCV cDNA exhibited polymorphism patterns in different families .

方法对80例前列腺癌冰冻组织（60例来自前列腺癌原发部位，20例来自盆腔淋巴结转移灶）中PTEN基因进行聚合酶链反应-单链构象多态性分析（PCR-SSCP），检测PTEN基因突变。

Methods : The PTEN gene mutations are investigated by PCR SSCP ( single strand conformation polymorphism analysis of polymerase chain reaction products ) from 80 frozen prostate tumor specimens .

目的：评价异源双链分析（HET）和单链构象多态性分析（SSCP）在基因突变检测中的作用。

Objective : To assess the roles of single stranded conformation polymorphism ( SSCP ) and heteroduplex polymorphism ( HET ) in screening gene mutation .

方法从80例行脊柱融合术患者的术前空腹静脉血中提取DNA，采用聚合酶链反应&单链构象多态性分析（PCR-SSCP）及测序技术，检测其BMP-2基因部分编码区及其侧翼序列的突变。

[ Methods ] Totally 80 patients were included in this study . DNA was extracted from the venous blood before operation and identified by single stranded conformation polymorphism ( SSCP ) analysis and direct sequencing .

对点8344、11778的PCR产物进行单链构象多态性分析（SSCP），并与患者亲属及健康人进行比较。

For point 8344 and 11778 PCR products , single strand conformation polymorphism ( SSCP ) was executed to detect mutations . The HA patients ' results of SSCP were compared with their relatives and volunteers ' .

方法：对102例胶质瘤石蜡切片PTEN基因进行PCRSSCP分析（聚合酶链反应单链构象多态性分析），检测PTEN基因突变；

Methods : The PTEN gene mutations were detected by PCR SSCP ( single strand conformation polymorphism analysis of polymerase chain reaction products ) in samplers of 102 archival , formalin fixed , paraffin embedded gliomas .

方法运用聚合酶链反应-单链构象多态性分析法（PCR-SSCP），对18例良性前列腺增生、37例前列腺腺癌组织进行雄激素受体基因CAG微卫星数量测定。

Methods The number of CAG microsatellites in exon 1 of the AR gene was measured in tissues of 18 cases of BPH and 37 of PC by polymerase chain reaction single strand conformation polymophism ( PCR SSCP ) analysis .

目的检测皮肤癌p53基因5～8外显子的突变频率，比较变性梯度凝胶电泳（DGGE）和单链构象多态性分析（SSCP）检测p53基因突变的敏感性。

Purpose To detect the mutation in the exon 5-8 of p53 gene in skin cancer by denaturing gradient gel electrophoresis ( DGGE ) and single strand conformation polymorphism ( SSCP ) . Comparing the sensitivity of DGGE and SSCP in screening mutations of p53 gene .

在确定SRY基因无大缺失情况下，用引物对A行单链构象多态性分析（PCR/SSCP），检测有无基因突变。

The results indicated the samples did not carry homozygous deletions in the SRY gene . The samples were further analyzed for the presence of subtle mutations by the PCR - SSCP ( single strand conformation polymerphism ) method with primers A.

方法采用巢氏-多聚酶联反应-单链构象多态性分析（NEST-PCR-SSCP）和DNA测序方法，对65例乳头状甲状腺癌和44例正常甲状腺组织TSHR3个胞内环基因进行检测。

Methods Three cytoplasmic loops of the TSHR were analyzed by an approach of combination of nest polymerase chain reaction and single strand conformation polymorphism ( NEST-PCR-SSCP ), followed by DNA sequencing in a total of 65 samples from papillary thyroid cancer patients and 44 controls .

方法对115例接种腮腺炎疫苗后再发生流行性腮腺炎的患儿血清，用单链构象多态性分析（PCR-SSCP）法分离得到流行株与疫苗株的核苷酸序列，分析其分子生物学特点。

Methods One hundred and fifteen serum samples of children with recurrence of epidemic parotitis after vaccination of parotitis vaccine were detected . The nucleotide sequences of the epidemic and vaccine strains were divided apart by PCR SSCP method , then molecular biological characters of both parotitis virus were analyzed .

异源双链分析和单链构象多态性分析在基因突变检测中的作用

Comparison of heteroduplex analysis and SSCP in gene mutation screening

毛细管电泳-单链构象多态性分析技术

Capillary electrophoresis-based single strand conformation polymorphism analysis

银染单链构象多态性分析&一种快速检测基因点突变的新方法

Silver stain single strand conformation polymorphism ── A rapid and simple method to detect gene point mutations