单链构象多态性

乏情不是一种疾病，而是一种多方面的体态迹象。临床常见病原菌16Srdna单链构象多态性分析

Anestrus ( absence of estrus ) is not a disease but a sign of a variety of conditions . 16S rDNA single-strand conformation polymorphism analysis of common clinical pathogenic bacteria

目的应用彗星试验（cometassay）、PCR-单链构象多态性（PCR-SSCP）分析和基因测序方法检测长期低浓度二硫化碳接触所致DNA损伤和基因突变等遗传毒性效应及其机制。

Objective To detect the genetic toxicity of carbon disulfide ( CS2 ) and its mechanism , including DNA damage and p53 gene mutation by comet assay , polymerase chain reaction single-strand conformation polymorphism ( PCR-SSCP ) and gene sequencing .

DNA单链构象多态性原理初探

Study on Principles of DNA Single Strand Conformation Polymorphism

进行基因位点DNA扩增聚合酶链反应-单链构象多态性分析。

Gene site DNA amplification was analyzed with polymerase chain reaction-single strand conformation polymorphism ( PCR-SSCP ) .

银染单链构象多态性技术检测原发性肝癌p53基因的突变

Detection of 53 gene mutation of PHC by SS SSCP

人肝癌组织中E-cadherin基因DNA单链构象多态性分析

SSCP analysis of E cadherin gene in human hepatocellular carcinomas

本文着重介绍了随机扩增多态性DNA、聚合酶链式反应-单链构象多态性、扩增片段长度多态性3种重要的DNA指纹技术在污染土壤诊断中的应用。

In this paper , the application of three main DNA fingerprints in eco-toxicological diagnosis of contaminated soil was discussed emphatically .

免疫荧光法与p53单链构象多态性分析检测胸水肿瘤细胞的结果分析

Analysis of tumor cells in thoracic liquid by fluorescence and p53 single-strand conformation polymorphism

PCR产物通过限制性酶切-单链构象多态性方法检测未知突变；

The PCR products were screened mutations using restriction endonuclease fingerprinting - single strand conformation polymorphism ( REF-SSCP );

HLA-A、B、C基因第二外显子的单链构象多态性分析及骨髓移植受供者基因配型

PCR-SSCP Analysis of Exon 2 of HLA-A , B and C Genes and Matching for Bone Marrow Donor-Recipient Pairs

单链构象多态性分析显示两病例组与对照组均出现3种不同的DNA带型，分别称为Ⅰ，Ⅱ，Ⅲ带型。

SSCP showed that 3 different DNA patterns , which were band patterns ⅰ, ⅱ, ⅲ respectively , were observed in all the 3 groups .

噬菌体生物扩增法、DNA序列分析及单链构象多态性分析检测结核分枝杆菌耐药性

Analysis and evaluation of phage amplified biologically assay , DNA sequencing analysis and single strand conformational polymorphism for the drug susceptibility testing of Mycobacterium tuberculosis

方法多聚酶链聚合反应单链构象多态性分析（PCRSSCP）、基因测序。

Methods Polymerase chain reaction - single strand confirmation polymorphism ( PCR-SSCP ), sequening .

临床常见病原菌16Srdna单链构象多态性分析

16S rDNA single-strand conformation polymorphism analysis of common clinical pathogenic bacteria

用PCR－单链构象多态性（SSCP）和DNA序列分析方法确定扩增序列突变情况。

Sequence variation was screened by means of PCR single stranded conformation polymorphism ( SSCP ) and DNA sequencing .

p53单链构象多态性变化与食管鳞癌患者术后生存期的关系

Relationship between single strain conformation polymorphism alteration of p53 and the survival rate in the patients with esophageal squamous cell carcinoma

聚合酶链式反应-单链构象多态性（PCR-SSCP）技术是一种简便、灵敏的突变检测方法。

PCR-SSCP is a simple and sensitive method to screen mutations .

方法运用双重对照PCR及单链构象多态性分析技术检测17例原发性膀胱癌。

Methods The status of MTS1 gene in17 cases of primary bladder cancer was detected by comparative multiple PCR and single-strand conformation polymorphism ( SSCP ) .

方法：聚合酶链反应（PCR）、单链构象多态性（SSCP）分析。

Methods : Polymerase chain reaction ( PCR ) and single strand conformation polymorphism ( SSCP ) was used .

人类免疫缺陷病毒gag基因的单链构象多态性分析

Analysis on single-strand conformation polymorphism of human immunodeficiency virus ( HIV-1 ) gag gene

单链构象多态性（SSCP）；

Single strand conformation polymorphism ( SSCP );

方法采用免疫组化和聚合酶链反应单链构象多态性技术（PCRSSCP）方法，检测82例GIST中CKIT蛋白的表达及CKIT基因exon11突变的情况。

Methods Immunohistochemical and PCR SSCP techniques were used to detect c kit protein expression and c kit gene exon 11 mutation in 82 patients with GIST .

方法应用PCR单链构象多态性分析技术，对胃癌患者的门静脉血、外周静脉血分别进行K-ras、p53基因检测，术后随访半年，了解肝转移情况。

Methods By PCR-SSCP K-ras and p53 gene mutation in portal vein blood and peripheral blood were detected in 62 patients with gastric carcinoma .

方法应用PCR及单链构象多态性（SSCP）分析技术结合基因序列测定方法确定突变类型。

Single strand conformation polymorphism ( SSCP ) essay and sequence analysis of the PCR product were used to ascertain the gene mutation .

肠杆菌4.5SRNA基因单链构象多态性的比较研究

Comparative study of single strand conformation polymorphism of 4 . 5S RNA gene in enterobacteria

用单链构象多态性分析（PCR-SSCP）对ING1基因进行突变检测；

Mutation analysis was detected by PCR-SSCP ( single-strand conformational polymorphism ) .

采用PCR单链构象多态性技术（SSCP）结合DNA序列测定证实，对病人SCN5A的全部28个外显子进行突变检测。

PCR and single strand conformation polymorphism analysis ( SSCP ) were combined with DNA sequencing confirmation to screen all28 exons of SCN5A gene .

方法采用RT-PCR法（逆转录DNA聚合酶链反应）、PCRSSCP（聚合酶链单链构象多态性分析）、DNA序列分析法对26例鼻咽癌组织及癌旁组织标本进行polβ基因突变研究。

Methods Twenty-six cases of nasopharyngeal carcinoma tissues and control epithelial tissues were collected to study DNA pol β gene mutation with PT-PCR , PCR-SSCP and DNA sequence analyzing technique .

方法设计三条针对结核分支杆菌的特异性引物，采用单管半套式PCR-单链构象多态性（SSCP）方法对结核分支杆菌、其它分支杆菌和富含GC碱基的细菌进行分析。

Methods Three specific primers to Mycobacterium tuberculosis were designed , Mycobacterium tuberculosis . Other Mycobacterium and GC-rich bacteria were analyzed by single-tube heminested PCR-SSCP technique .

26Srdna单链构象多态性分析在临床酵母菌菌种鉴定中的应用

Rapid identification of clinical yeast species by single-strand conformation polymorphism analysis of 26S rDNA D1 / D2 domain