单菌落

待反应器去除硝酸盐效果稳定后，取膜，稀释涂布平板，挑取单菌落纯化培养。

After the reactor shows a stability of nitrate remove effect , get the biofilm , dilution the biofilm solution and spread plates , then pick a single colony cultured .

以广东省九江酒厂有限公司提供的小曲为出发菌，采用土豆汁培养基进行纯培养，选取长有2～4个单菌落的平板进行分离纯化，制成纯培养菌。

Xiaoqu provided by Guangdong Jiujiang Distillery was used as startup bacteria for pure culture by potato juice culture medium , then 2 ~ 4 single colony plates were selected for separation and purification to produce pure culture bacteria .

单菌落PCR法直接快速鉴定重组克隆

Rapid Characterization of Recombination Clone by PCR Screening of Individual Bacterial Colonies

结果表明，单菌落PCR法是一个有效简便的鉴定重组阳性克隆的方法。

It showed that the method individual bacterial colonies PCR was an efficient , easy one that characterized recombination clones .

以K2型嗜杀酵母为材料在改进Russell（1986）的嗜杀活性检测方法基础上，建立了双层平板单菌落检测法。

On foundation of improving Russell 's method , with K_2 killer yeast , dilayer plate single colony assay for killer strain was set up .

从月季品种金玛丽、曼海姆、杏花村、梅郎口红的根部肿瘤组织中纯化了9株分离物，根据其在MW选择性培养基上的单菌落形态初步判断其为根癌土壤杆菌。

Nine isolates were purified from the crown galls on rose plants and were characterized as Agrobacterium tumefaciens strains according to the colony morphology on the selective MW medium .

挑选单菌落，PCR鉴定阳性克隆，用0.5%甲醇诱导表达，并利用SDS-PAGE及Western-Blot鉴定表达产物。

After identified by PCR , the positive transformants were induced by 0.5 % methanol , and the expression products were detected by SDS-PAGE and Western-Blot .

同时，单菌落PCR法也可应用于重组质粒转化后的农杆菌的筛选，单菌落PCR法的扩增结果和农杆菌液扩增的结果一致。

The selecting of agrobacterium transformed with recombination plasmid could also use this method of PCR screening of individual bacterial colonies . The result of individual bacterial colonies PCR was as well as that of PCR using bacterial solution as template .

随机挑选单菌落小规模培养，碱裂解法提取质粒DNA，PstⅠ／BglⅡ双酶切后行变性聚丙烯酰胺凝胶电泳和银染分析，筛选含有不同等位基因的重组质粒后测序。

Use alkaline lysis method to extract the plasmid DNA . DNA constructs were identified by Pst I / Bgl II digestion , denaturing polyacrylamide gel electrophoresis and DNA sequence analysis .

挑取白色单菌落接种扩增后提取质粒。

Take white single colonies for extraction plasmid after vaccination and amplification .

在固体培养基上挑单菌落放入液体培养基进行纯化。

The single Mh colony was picked into liquid medium to purify .

经α互补检测，初步筛选重组子。随机挑取单菌落分别培养，提取质粒，经琼脂糖凝胶电泳和斑点杂交，最后确定重组子，重组率为43.8%。

Recombinants were determined by means of α - complementary test , agarose gel electrophoresis and dot hybridization .

用未经处理的含酚焦化工业废水为基础配制培养基，采用平板涂布法，在10~（-5）稀释度下得到100个单菌落分离物。

100 isolates were obtained from feed water solid media using spread-plate method with diluted activated sludge ( 10 - 5 dilution rate ) .

通过对海水中的细菌进行分离，培养，得到两种不同形态的单菌落：一种是圆形、隆起、边缘呈波状的菌落，一种是点状、扁平、边缘基本整齐的菌落。

Two different colonies were gained by separating and incubating the bacteria in seawater . One is round , ridgy and has flexuous brim , the other is punctuate , compressed and has orderly brim .

研究了单孢分离物菌落形态和菌丝生长速率。

The biological characteristics of growth rate and colony morphology of single – zoospore isolates were studied .