单菌落
- 网络single colony
![单菌落](../cache/1186112.jpg)
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待反应器去除硝酸盐效果稳定后,取膜,稀释涂布平板,挑取单菌落纯化培养。
After the reactor shows a stability of nitrate remove effect , get the biofilm , dilution the biofilm solution and spread plates , then pick a single colony cultured .
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以广东省九江酒厂有限公司提供的小曲为出发菌,采用土豆汁培养基进行纯培养,选取长有2~4个单菌落的平板进行分离纯化,制成纯培养菌。
Xiaoqu provided by Guangdong Jiujiang Distillery was used as startup bacteria for pure culture by potato juice culture medium , then 2 ~ 4 single colony plates were selected for separation and purification to produce pure culture bacteria .
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单菌落PCR法直接快速鉴定重组克隆
Rapid Characterization of Recombination Clone by PCR Screening of Individual Bacterial Colonies
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结果表明,单菌落PCR法是一个有效简便的鉴定重组阳性克隆的方法。
It showed that the method individual bacterial colonies PCR was an efficient , easy one that characterized recombination clones .
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以K2型嗜杀酵母为材料在改进Russell(1986)的嗜杀活性检测方法基础上,建立了双层平板单菌落检测法。
On foundation of improving Russell 's method , with K_2 killer yeast , dilayer plate single colony assay for killer strain was set up .
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从月季品种金玛丽、曼海姆、杏花村、梅郎口红的根部肿瘤组织中纯化了9株分离物,根据其在MW选择性培养基上的单菌落形态初步判断其为根癌土壤杆菌。
Nine isolates were purified from the crown galls on rose plants and were characterized as Agrobacterium tumefaciens strains according to the colony morphology on the selective MW medium .
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挑选单菌落,PCR鉴定阳性克隆,用0.5%甲醇诱导表达,并利用SDS-PAGE及Western-Blot鉴定表达产物。
After identified by PCR , the positive transformants were induced by 0.5 % methanol , and the expression products were detected by SDS-PAGE and Western-Blot .
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同时,单菌落PCR法也可应用于重组质粒转化后的农杆菌的筛选,单菌落PCR法的扩增结果和农杆菌液扩增的结果一致。
The selecting of agrobacterium transformed with recombination plasmid could also use this method of PCR screening of individual bacterial colonies . The result of individual bacterial colonies PCR was as well as that of PCR using bacterial solution as template .
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随机挑选单菌落小规模培养,碱裂解法提取质粒DNA,PstⅠ/BglⅡ双酶切后行变性聚丙烯酰胺凝胶电泳和银染分析,筛选含有不同等位基因的重组质粒后测序。
Use alkaline lysis method to extract the plasmid DNA . DNA constructs were identified by Pst I / Bgl II digestion , denaturing polyacrylamide gel electrophoresis and DNA sequence analysis .
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挑取白色单菌落接种扩增后提取质粒。
Take white single colonies for extraction plasmid after vaccination and amplification .
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在固体培养基上挑单菌落放入液体培养基进行纯化。
The single Mh colony was picked into liquid medium to purify .
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经α互补检测,初步筛选重组子。随机挑取单菌落分别培养,提取质粒,经琼脂糖凝胶电泳和斑点杂交,最后确定重组子,重组率为43.8%。
Recombinants were determined by means of α - complementary test , agarose gel electrophoresis and dot hybridization .
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用未经处理的含酚焦化工业废水为基础配制培养基,采用平板涂布法,在10~(-5)稀释度下得到100个单菌落分离物。
100 isolates were obtained from feed water solid media using spread-plate method with diluted activated sludge ( 10 - 5 dilution rate ) .
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通过对海水中的细菌进行分离,培养,得到两种不同形态的单菌落:一种是圆形、隆起、边缘呈波状的菌落,一种是点状、扁平、边缘基本整齐的菌落。
Two different colonies were gained by separating and incubating the bacteria in seawater . One is round , ridgy and has flexuous brim , the other is punctuate , compressed and has orderly brim .
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研究了单孢分离物菌落形态和菌丝生长速率。
The biological characteristics of growth rate and colony morphology of single – zoospore isolates were studied .