化学酶

第一种方法是以pH敏感的水溶性线性高分子作为固相载体的化学酶法合成技术。

Firstly we utilized a pH-responsive water-soluble polymer as support in chemo-enzyme synthesis .

化学酶法合成RGD三肽的研究

Study on the Synthesis of RGD Tripeptide by the Combination of Chemical and Enzymatic Method

方法：应用单克隆抗体免疫组织化学酶标法对确诊为IgA肾病患者的肾组织进行染色，依据沉积类型并按半定量方法结合病情进行分析。

Methods : Renal tissue staining was performed by immunohistochemical method in patients with IgA nephropathy , results were analyzed according to deposition pattern and semiquantitative determination in association with condition of the disease .

原代培养脐静脉内皮细胞和诱导前、后的GSCs作为对照，用化学酶法检测并对比三组细胞培养液中一氧化氮的含量。

We primary cultured the normal umbilical vein endothelial cells and made it as a contrast with the pre-induced and post induced GSCs , NO in the medium of the three parts were measured with the chemical enzymatic method and compared with each other .

防衰老与生物化学酶&记程一桂与他的科研成果衡态系列功能型蛋白质粉

Prevention of Senility & Bio - chemical Ferment

5-取代海因的消旋过程对化学酶法制备D-氨基酸的影响

Study on the Racemization of 5-Substituted Hydantoin in the Bioconversion of Hydantoin Catalyzed by Hydantoinase

方法：采用免疫细胞（组织）化学酶染色法及免疫荧光技术研究带3样蛋白在心肌细胞的定位，第一抗体为红细胞带3蛋白单克隆抗体。

Methods : The localization of band 3 like proteins in the myocardial cells was determined with immunocytochemical enzyme staining and immunofluorescence in the adult rats . Monoclonal antibody against erythroid band 3 proteins was used as the primary antibody .

结果：免疫化学酶染色显示带3样蛋白存在于成年大鼠心肌细胞膜及膜的皱褶或折入处，免疫荧光法显示带3蛋白单克隆抗体标记了心肌细胞膜和肌纤维横纹结构的部位。

Results : Enzyme staining revealed the presence of band 3 like proteins in the sarcolemma of cultured adult rat myocardial cells and invaginations into the cells . Immunofluorescence demonstrated that the membrane and transversal riblike structures in the myocardial cells were labeled by the monoclonal antibody .

本研究对于遗传工程中的化学核酸酶以及以DNA为靶标的药物设计有重要的意义。

These studies are very important to the design of chemical nucleases and DNA-targeting drugs .

人促甲状腺激素（TSH）芯片磁分离化学发光酶免疫分析法

Biochip Magnetic Chemiluminescence Enzyme Immunoassay for Rapid , Sensitive Determination of Thyrotropin

低分子肝素（lowmolecularweightheparin，LMWH）是未分级肝素经化学或酶法解聚而得，相对分子质量为4000～6500。

Low molecular weight heparin ( LMWH ) was obtained by chemical or enzymatic digestion of heparin . Its relative molecular weight was from 4000 Dalton to 6500 Dalton .

切片应用HRP与免疫细胞化学过氧化物酶抗过氧化物酶复合物法相结合的方法相结合法显色，确定HRP标记细胞的性质。

Combining method of HRP retrograde tracer with immunocytochemistry stained the sections to determine the nature of HRP labeled cells .

本研究分别建立了检测玉米中ZEN的化学发光酶免疫分析法及生物素-亲和素酶联免疫分析法。

In this study , chemiluminescence analysis and biotin-avidin system were used to establish CLEIA and BA-ELISA for detecting ZEN in corn .

本文综述了化学核酸酶的研究新进展。讨论了化学核酸酶模型催化磷酸酯的水解反应，DNA和RNA的裂解和水解等反应，并对它催化各类反应的作用机制进行了评述。

The recent advance in chemical nuclease is reviewed , and the mechanisms of hydrolysis or cleavage of phosphate ester . DNA and RNA catalyzed by chemical nuclease are discussed by the way .

采用固相化学发光酶免疫分析法（ICMA法）检测TSH，对于TSH在正常范围外的人群，检测游离三碘甲状腺原氨酸（fT3）和游离甲状腺激素（fT4）。

TSH , free triiodothyronine ( fT_3 ) and free thyroxin ( fT_4 ) were measured by chemiluminescence immunoassay .

在有机溶剂中化学-酶法合成Bz-RGD-（NH2）2-细胞粘附位点RGD三肽前体

Chemo-enzymatic Synthesis of Bz-RGD - ( NH_2 ) _2 , the Precursor of Cell Adhesive Motif RGD Tripeptide in Organic Solvents

化学&酶法合成细胞粘附位点RGDS前体四肽Bz-RGDS-NH2

Chemo-enzymatic Synthesis of Bz-RGDS-NH_2 , the Precursor of Cellular Adhesion Motif RGDS Tetrapeptide

本文首次提出了偶合反应化学发光酶联免疾分析测定HRP的新方法，系统地分析和研究了偶合反应在化学发光酶联免疫分析中的应用；

A method for the determination of horse radish peroxidese ( HRP ) and its application were investigated by coupled reaction-Chemiluminescence enzyme immunoassay ( CLEIA ) .

应用免疫组织化学链酶菌抗生素蛋白-过氧化物酶法（Streptavidin-perosidase，SP法），结合葡萄糖氧化酶-DAB-硫酸镍胺呈色。

Using immunohistochemical Streptavidin-Perosidase ( SP ) method together with glucose oxidase-DAB-nickel the visualization was fulfilled .

方法采用深度烧伤患者愈后增生性瘢痕的水泡液，以化学发光酶免疫法测定患者血和瘢痕水泡液中TNF，IL-6的含量，监测瘢痕早期组织中TNF，IL-6的变化。

METHODS : The blisters of hyperplastic scars were obtained from the patients with deep burn after recovery . The contents of TNF and IL 6 in blood and blisters were tested with chemiluminoimmunoassay .

胸腺五肽（TP-5）的新型化学&酶法合成研究

Study on the Synthesis of Thymopentin ( TP-5 ) by a Novel Chemo-enzymatic Method

方法：研究对象为正常对照组36例，稳定型心绞痛组32例，心肌梗塞组39例，采用化学发光酶分析法检测其血清TNF-α、IL-1β、IL-6、IL-8水平。

Methods Serum levels of TNF - α, IL-1 β, IL-6 and IL-8 levels were determined by chemiluminescence immunoassay in 32 patients with stable angina , 39 patients with acute myocardial infarction and 36 controls .

化学与酶促相结合合成并克隆核糖体失活蛋白Gelonin基因

Synthesis and Clone of Ribosome Inactivating Protein-Gelonin Gene by the Combination of Chemical and Enzymatic Method

方法：采用化学发光酶免疫分析法检测50例重症感染新生儿及40例正常健康儿童血清IL6、IL8水平。

Methods : Serum IL 6 and IL 8 were measured with chemiluminescent enzyme immunoassay in 50 newborns with severe infection and in 40 healthy infants .

方法利用免疫细胞化学、酶联免疫吸附测定等方法，检测高浓度Ca2+作用下，培养的小鼠分化表皮角质形成细胞表面及培养上清中tPA表达的变化。

Methods The immunocytochemical method ( ICC ) and enzyme linked immunosorbent assay ( ELISA ) were used to determine the tPA amounts in culture supernatant as well as on cell surface of the differentiated mouse KCs cultured in high calcium medium .

偶合反应化学发光酶联免疫分析检测反应新体系的探讨

New System of Analytical Reaction in Chemiluminescence Enzyme Immunoassay With Coupled Reaction

人表皮生长因子基因化学及酶促合成与克隆

Chemical and enzymatic synthesis and cloning of human epidermal growth factor gene

化学修饰酶在天然药物研究和生物技术中的应用

Chemical Dressing Enzyme : Its Application in Researches on Natural Drugs and Biotechnology

谈生物化学中酶化学的教学

On the Teaching of Enzyme Chemistry in Bio chemistry

第一部分研究了化学-酶二步法合成蔗糖-6-月桂酸酯。

First , the chemo-enzymatic synthesis of6-O-lauroylsucrose was researched .