内细胞团

无血清培养山羊内细胞团的研究

The study of serum-free culture of goat inner cell mass

胚胎干细胞（Embryonicstemcell，EScell）是来源于受精卵内细胞团（innercellmass，ICM）的多能细胞。

Embryonic stem ( ES ) cells are pluripotent cells derived from inner cell mass ( ICM ) of fertilized eggs .

传统的胚胎干细胞（ES）多从囊胚的内细胞团（ICM）获得，如小鼠ES。

Traditional embryonic stem cells ( ES ) are derived from the blastocyst inner cell mass ( ICM ), such as mouse ES .

特别是继1998年JamesThomson博士建立人ES细胞系之后，世界上其他许多科学家也相继成功地从囊胚内细胞团分离并建立了ES细胞系。

Especially after doctor James Thomson established human ES cell lines , others scientists in the world also cultured ES lines from inner cell mass ( ICM ) .

添加胰岛素、白血病抑制因子和β-巯基乙醇均利于抑制ES的分化和促进内细胞团的增殖。

The media supplied respectively with insulin , leukaemia inhibitory factor and β - mercaptoethanol were good at inhibiting the differentiation of ES-like cells and promoting reproduction of ICM .

体外培养第7天的囊胚的内细胞团细胞（ICM）与滋养层细胞数平均为37和47，ICM占44.2%。

The average cell number of inner cell mass and trophoblast was 37 and 47 respectively , in which 44.2 % was inner cell mass .

当囊胚由透明带自然孵出后，用细玻璃针剥离囊胚中的内细胞团（innercellmass，ICM）并与饲养细胞进行共培养。

After the blastocysts were hatched naturally from the zone pellucida , the inner cell mass ( ICM ) was dislodged from blastocysts using the sealed end of a finely drawn Pasteur pipette and co cultured with the feeder cells .

方法从KM小鼠囊胚的内细胞团中分离培养胚胎干细胞，进行近交系C57BL6J小鼠受体囊胚腔内注射，进行嵌合体构建实验。

Methods We isolated embryonic stem cells from inner cell mass of KM mice blastocysts . Then we transferred embryonic stem cells into blastocoele of C57BL / 6J inbred mice by microinjection in order to construct chimeric mice .

结果表明，囊胚期胚胎不除去透明带而直接培养产生胚胎干细胞，初次克隆率为55%；胰酶法去透明带分离的内细胞团（ICM）培养产生胚胎干细胞，初次克隆率为90%。

The results showed that : the primary colony rates of bovine embryonic stem cell from normal IVF blastocysts and trypsin-isolated ICMs were 55 % and 90 % , respectively .

注入嵌合法采用机械剥离法或免疫外科法分离胚胎内细胞团（ICM），然后注入到已去除ICM的受体囊胚中构建水牛和黄牛的嵌合胚；

Bovine inner cell mass ( ICM ) was isolated from blastocysts by either mechanical method or immunosurgery , and then injected into buffalo blastocysts that ICM was removed to reconstruct a chimeric embryo .

结果表明：1添加10ng/mlLIF能提高胚胎贴壁率，促进内细胞团（ICM）和ES细胞的增殖并抑制其分化；

The results showed that : ① Supplementing with 10 ng / mL LIF could enhance the ration of embryo attachment , promote the proliferation of ICM and ES cells and inhibit the differentiation of ICM and ES cells ;

胚胎干细胞（ES）是一种来源于胚胎内细胞团的全能型干细胞，具有分化为各种组织细胞的潜能，在适宜的条件下可分化为人们需要的细胞用于某些疾病的治疗。

Embryonic stem cells ( ES ) are totipotent stem cells originated from inner cell mass of early embryos . They possess the capabilities to differentiate into various cell and tissue types desired , which can be used for cell or tissue-based therapy to cure some diseases .

随机选取其中各种孵出状态的囊胚进行免疫染色，再通过共聚焦显微镜观看内细胞团（ICM）信号状态，记数ICM形态正常、分散、分裂的比例。

To immunostain randomly which selected from all kinds of hatched blastocysts , watch the states of inner cell mass ( ICM ) signals with the confocal microscope and record the ratio of ICM of normal morphology , dispersion and division .

人胚胎干细胞（ESC）起源于囊胚的内细胞团，而人胚胎生殖细胞（EGC）由发育后期的原始生殖细胞（PGC）发育而来。

ESCs are derived from the inner cell mass of pre-implantation blastocysts , whereas embryonic germ cells ( EGCs ) are derived from the primordial germ cells ( PGCs ) of embryos in later stages of development .

结果显示：消化掉外层粘蛋白和部分透明带的胚胎96h贴壁率（85.29%，29/34）较高，内细胞团生长良好；

Results : There was high rate of attachment of blastocysts that were indigested external mucoprotein and partial zona pellucida in 96 h culture ( 85.29 % , 29 / 34 ) and growth of ICM was excellent ;

ESCs来源于哺乳动物的囊胚内细胞团和原始生殖细胞，具有全能分化和无限增殖两大特性。

Embryonic stem cells ( ESCs ) were isolated from inner cell mass of mammalian blastocysts and primordial germ cells , which had two major characteristics , one is unlimited proliferation , another is the capcity of differentiation into all cell types .

胚胎干细胞（ESC）建系取材包括桑椹胚的卵裂球、囊胚的内细胞团（ICM）、上胚层细胞和原始生殖细胞（PGCs），甚至从新生鼠睾丸细胞也分离得到ES样细胞。

The inner cell mass ( ICM ), blastomeres , epiblasts and primordial germ cells ( PGCs ) are usually used as primary materials for the establishment of embryonic stem cell ( ESC ) lines . ES-like cells have even been isolated from neonatal mouse testis .

通常用来源于囊胚期胚胎的内细胞团的胚胎干细胞、通过核移植得到的胚胎干细胞以及在成体细胞中转入外源转录因子得到的iPSCs，进行体细胞重编程研究。

IPSCs obtained from embryonic stem cells in the inner cell mass of blastocysts , the nuclear transfered embryonic stem cells and the exogenous transcription factor transferred somatic cells were used to conduct the somatic cell reprogramming research .

人胚胎干细胞（hESC）是一类来源于植入前囊胚内细胞团（ICM），具有体外无限增殖潜能且能在特定条件下分化为多类成体组织或胚外组织的细胞群体。

Human embryonic stem cells ( hESCs ), derived from inner cell mass ( ICM ) of preimplantation-stage embryos , possess dual capability to self-renew indefinitely in vitro or give rise to a wide variety of somatic and extraembryonic tissues under certain conditions .

一种简单的分离猪囊胚内细胞团的方法

A Simple Method of Isolating Inner Cell Mass from Porcine Blastocyst

由猪囊胚内细胞团分离胚胎干细胞的研究

Isolation of embryonic stem cells from inner cell mass of porcine blastocysts

用囊胚内细胞团建立小鼠胚胎干细胞系的尝试

Establishment of embryonic stem cell line derived from inner cell mass of mouse

孵化囊胚贴壁增殖，内细胞团呈圆柱状生长。

The blastula cell attached and proliferated , inner cell mass presented columned growth .

由不同发育时期内细胞团构建的嵌合兔

Construction of chimeric rabbits by blastocyst injection of ICM cells from different developmental stages

小鼠内细胞团标志蛋白的性质、分离和纯化

Isolation , purification and characterization of the inner cell mass marker protein of the mouse embryo

另外，某些细胞因子对内细胞团细胞增殖有影响。

Furthermore , cytokine and growth factor influence the proliferation of cells of inner cell mass .

胚胎干细胞是来自于囊胚内细胞团的多能性细胞。

ES cells are pluripotent stem cells , which are derived from inner cell mass of blastocyst .

不同饲养层细胞对胚胎干细胞和内细胞团的影响

Effect of Different Feeder Layers on Mouse Embryonic Stem Cells and Inner Cell Mass of Rabbit Blastocysts

对孵化出的内细胞团进行消化，然后接种培养。

After 4 days , ICMs ( inner cell mass ) were trypsinized , seeded and cultured .

胚胎干细胞来源于哺乳动物囊胚内细胞团。

Embryonic stem cells ( ESCs ) is derived from the inner cell mass of mammalian blastocysts .