内参

内参法在线粒体DNA纯度鉴定中的应用

Application of Internal Reference Method in Identification of Mitochondrial DNA Purity

用单晶X衍射测定了酮咯酸脯氨酸苄酯酰胺一个异构体的晶体结构，并用内参法确定了其绝对构型。

The crystal structure of one diastereoisomer of the amides was determined by single crystal diffraction analysis , and the absolute configuration of which was deduced by internal reference .

老年大鼠组织mRNA分析中内参基因的选择

Reference genes selection in mRNA analysis of aging rat tissues

甘蔗基因表达定量PCR分析中内参基因的选择

Selection of Control Genes in Real-time qPCR Analysis of Gene Expression in Sugarcane

竞争性PCR内参照菌的构建及应用

Construction and application of E. coli strains used as internal control for competitive PCR

目的构建竞争性定量PCR检测幽门螺杆菌（Hp）cagA基因的内参标模板并做序列测定。

AIM To construct an internal standard template for quantitative PCR detection of cagA + Hp .

2>在进行RT-PCR半定量测定某种成分时可以通过设计内参照物的方法进行半定量分析；

2 > We can take a RT-PCR semi-fix quantification analysis by the means of designing inner reference ;

可变内参比法ICP-AES同时测定钢铁中六个微量元素

Determination of six trace elements in steels by ICP AES with the changed internal standard

定量检测HpCagA基因内参标模板的构建及测序

Construction and sequencing of an internal standard template for quantitative PCR detection of cagA Hp

由于β-actin在瘢痕组织和正常皮肤中的不恒定性，建议在瘢痕的mRNA定量研究中不作为内参照物。

In scar mRNA quantitative research , β - actin is not suitable to be the reference standard because it fluctuates between the scar and normal skin .

在确定的体系中，HPV扩增区及内参片段能得到强扩增。

In the determined system , HPV amplification area and the internal reference fragments can be strongly amplified . 3 .

猪组织中miR-103实时定量PCR分析时合适内参的确定

Identification of Suitable Reference for Quantitative RT-PCR Assays of miR-103 in Pig Tissues

以cDNA为内参标RT-PCR定量检测心肌细胞内p53和Bcl-2基因mRNA表达量的方法研究

Study of method assessment of p53 and Bcl-2 gene mRNA expression in rabbit myocardial cells by reverse transcriptase-polymerase chain reaction using cDNA as an internal standard

随机挑选2个模板用普通PCR法对50对引物进行筛选，结果有26个候选基因及内参基因的引物扩增条带清晰、唯一。

Two cDNA templates of carp were randomly picked and amplified by a set of 50 primers , Fortunately , 26 candidate genes can be amplified with only one clear bands .

利用qRT-PCR技术，以Tubulin为内参基因，我们检测了miRNA靶基因的表达。

We detected the expression levels of miRNA targets by qRT-PCR with tubulin as internal control .

应用RTPCR法和RNA酶保护试验（RPA）法测定2种NOS的表达，以2,3二羟基丙醛3磷酸脱氢酶（GAPDH）为内参。

NOS expression was detected by using RT PCR and RNAase protection assay ( RPA ), and GAPDH was used as an internal standard .

内参引物PC01/02用于扩增人β-Globin基因，对样本提取及PCR过程进行质控，产物大小为221bp。

Internal reference primer PC01 / 02 was used to amplification of human β - Globin gene and quality control of the process of sample extraction and PCR . Its product was 221 bp .

海带配子体18SRRNA基因的克隆、序列分析及其作为内参基因的应用

Cloning and Sequence Analysis of 18S rRNA Gene from Laminaria japonica Gametophytes and Its Application as an Internal Standard

结果3例前列腺癌组织及1例前列腺癌细胞株中聚集素与内参基因βactin相比较的相对表达量明显高于3例正常前列腺组织。

Results Compared with the internal marker gene β actin , the expression level of clusterin in prostate cancer is much higher than that of normal prostate .

结论内参标模板rfcagA构建，对竞争性定量PCR检测HpCagA基因的方法的建立具有重要价值。

CONCLUSION The construction of an internal standard template ( rfcagA ) has a high value for quantitative PCR detection of cagA + Hp .

另外应用逆转录聚合酶链式反应（RT-PCR）法以β-肌动蛋白为内参，检测饲养60d的獭兔肝脏中GPxmRNA的表达量。

In addition , the expression amounts of mRNA from GPX in livers of rex rabbits after 60 days was detected with RT-PCR set by an inner parameters of p-actin .

骨髓移植4,8,12,16周组分别于对应时间点处死，取其腓肠肌，制备抗肌萎缩蛋白样品，进行抗肌萎缩蛋白westernblot免疫印迹分析，以GAPDH作为内参。

The mice in 4 transplantation groups were killed at each time point , and the gastrocnemius was harvested to prepare dystrophin samples . The amount of dystrophin expression was detected by Western blot with GAPDH as control .

目的：引入细胞管家基因（β-actin）作为多重扩增的内参照物以达到资料比较的标准化，用于研究c-fos在甲状腺乳头状癌中的表达。

Objective : β - actin , one of housekeeping genes , was used in multiple PCR as the internal reference to normalize the data in the study of c-fos expression in thyroid papillary carcinoma .

【方法】已克隆S-腺苷蛋氨酸脱羧酶、鸟氨酸氨基转移酶、甜菜碱脱氢酶3个基因和内参基因的基础上，应用定量PCR技术，对这3个基因在不同胁迫时间下的表达水平进行分析。

[ METHOD ] In this paper , three gene S-adenosylmethionine decarboxylase ( SAMDC ), ornithine aminotransferase ( OAT ) and betaine aldehyde dehydrogenase ( BADH ) plus one house-keeping gene had been cloned and their expression levels under different stresses were evaluated by real-time PCR technique .

本实验建立的鸭β-actin基因实时荧光定量PCR法扩增效率高、线性范围广、检测周期短，为β-actin基因作为内参基因进行鸭功能基因与病原基因表达的定量分析奠定了基础。

The real-time PCR assay developed in this study can detect β - actin in broad range with high efficiency and short time . The assay provide the basis for β - actin gene of duck as a reference gene in quantitative analysis of duck mRNA expression .

同时，以β-actin为内参照物，进行RT-PCR半定量分析，比较精子受精抗原（FA-1）在睾丸、附睾头、体、尾组织中的表达量。

Meanwhile , semi-quantitative analysis of FA-1 expression in testis and caput , corpus , cauda epididymis was carried out by RT-PCR based on β - actin as an inner control .

因此，本研究为猪的基因表达转录分析qPCR提供了几组较为可靠的内参基因组合方案，可用来比较准确地校正目标基因的表达量。

Therefore , this study will provide some more reliable reference gene combinations for qPCR of gene expression studies in pig tissues and skeletal muscle development .

主要试剂：血液稀释液、溶血液、Bcr／Abl（P230）和内参GAPDH基因引物及探针、DCC基因引物、RNA提取液、cDNA合成试剂盒，乙酸钠-柠檬酸缓冲液（pH：4.3）为流动相。

Main reagent : dilution fluid , hemolysis fluid , Bcr / Abl ( P230 ) , primer and probe of GAPDH gene , primer of DCC gene , RNA extracting fluid , cDNA kit .

RTPCR结果证明在17例正常前列腺移行带和20例良性前列腺增生尿道周围组织中，EGF与内参基因β肌动蛋白的相对表达量明显高于外周带。

RT-PCR proved that in comparison with the expression of the internal marker β - actin gene , the expression levels of EGF in the normal transitional zone and in the periurethral zone from BPH were markedly higher than that in the peripheral zone .

以苦荞持家基因H3为内参基因，采用半定量RT-PCR技术检测Fls基因在苦荞花期茎、叶和花中的表达量。

Using the housekeeping gene Histone 3 ( H3 ) from F. tataricum as reference gene , semi-quantitative RT-PCR was performed to detect the expression of Fls gene in stems , leaves and flowers during florescence .